高速液体クロマトグラフ法による医薬品の分析(第2報) : 生薬中のベルベリンの迅速定量

抄録

A rapid estimation procedure using a high-speed liquid chromatography is described for determination of berberine in crude drugs (phellodendron cortex, coptis rhizome, and phellodendron extract). Separation can be achieved within 10 min employing a 1 m column packed with Zipax SCX, using 0.1 M NaClO₄ (CH₃CN-H₂O 6 : 4), and 0.2M H₃BO₃-0.002 M NaClO₄ (pH 8.5) (7 : 3) solution as an eluant in a Du Pont LC 830 liquid chromatograph. Berberine in crude drugs was extracted with citric acid-methanol solution and injected into the column. Accuracy in this method was found to be less than ±1% with the relation Y=13.806X+0.0025, where X=quantity of berberine (μg) and Y=peak height of berberine (cm), and a relative coefficient of 0.999.