On a way of structural analysis of total
N-glycans linked to glycoproteins in royal jelly (Kimura, Y.
et al.,
Biosci. Biotechnol. Biochem., 64, 2109-2120 (2000), Kimura, M.
et al.,
Biosci. Biotechnol. Biochem., 66, 1985-1989 (2002)), we found that some complex type
N-glycans containing a β1-3galactose residue occur on the insect glycoproteins. Up to date, it has been considered that naturally occurring insect glycoproteins do not bear the galactose-containing
N-glycans, therefore, in this report we describe the structural analysis of the complex type
N-glycans of royal jelly glycoproteins.
By a combination of endo- and exo-glycosidase digestions, IS-MS analysis, and
1H-NMR spectroscopy, the structures of the β1-3 galactose-containing
N-glycan were identified as the following; GlcNAcβ1-2Manα1-6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc, Manα1-3Manα1-6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc, and Manα1-6(Manα1-3)Manα1-6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc. To our knowledge, this is the first report showing that the Galβ1-3GlcNAcβ1-4Man unit occurs in
N-glycans of insect glycoproteins, indicating a β1-3 galactosyl transferase and β1-4GlcNAc transferase (GNT-IV) are expressed in the honeybee cells.
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