Bioscience and Microflora
Online ISSN : 1349-8355
Print ISSN : 1342-1441
ISSN-L : 1342-1441
28 巻 , 1 号
選択された号の論文の3件中1~3を表示しています
Full Paper
  • Jasna Mrvcic, Damir Stanzer, Visnja Bacun-Druzina, Vesna Stehlik-Tomas
    原稿種別: Full Paper
    2009 年 28 巻 1 号 p. 1-6
    発行日: 2009年
    公開日: 2009/02/17
    ジャーナル フリー
    Western diet is frequently low in essential metal ions. A common method for preventing metal ion deficiency is pharmacological supplementation, especially in a highly available form such as metalo-protein complexes. Accordingly, in this work, specific lactic acid bacteria were assessed for their ability to bind copper ions. Significant amounts of copper ions were bound, and the binding potential was found to be strain specific. Differences among the strains were evaluated with the Langmuir model for biosorption. Binding of copper was a fast process, strongly influenced by ionic strength, pH and biomass concentration. During the process, copper ions significantly reduced cell viability. Discharge of copper ions in a simulated gastrointestinal tract was examined; 85-90% of copper ions bound to LAB were discharged in the gastrointestinal model system.
  • Fumiaki Abe, Tomoko Yaeshima, Keiji Iwatsuki
    原稿種別: Full Paper
    2009 年 28 巻 1 号 p. 7-15
    発行日: 2009年
    公開日: 2009/02/17
    ジャーナル フリー
    The safety of two probiotic bifidobacterial strains, Bifidobacterium breve M-16V and Bifidobacterium infantis M-63, was evaluated by single dose and 90-day repeated dose oral toxicity tests using rats. In the single dose oral toxicity test using 1.4 × 1012 CFU/kg of B. breve M-16V or 3.2 × 1011 CFU/kg of B. infantis M-63, there were no death and no abnormalities. In the 90-day repeated dose oral toxicity test using 2.3 × 1011 CFU/kg/day of B. breve M-16V or 7.6 × 1010 CFU/kg/day of B. infantis M-63, no death and no abnormalities in body weight, food consumption, water consumption, urinalysis, hematology, blood biochemistry, organ weights, and histophathological findings were observed. The acceptable daily intake (ADI) of B. breve M-16V was calculated to be 1.38 × 1011 CFU/day for an adult weighing 60 kg and 1.15 × 1010 CFU/day for an infant weighing 5,000 g. Based on the findings of the present study together with the taxonomy and distribution data, as well as clinical experience in preterm infants, B. breve M-16V may be considered as extremely safe for consumption by humans including infants. Although B. infantis M-63 was considered as safe as B. breve M-16V, further confirmation by clinical investigations may be required.
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