In the European Standard EN71 on the ‶Safety of Toys‶, 16 kinds of the coloring agents and 9 kinds of the
aromatic primary amines for the dyes used in the toys made of textiles are regulated. By adding an
artificial saliva, we conducted the dissolution test of the artificial sweat stipulated in ‶the color fastness test
to a sweat of JIS (JIS L 0801:2004)‶ that was equivalent to the early test of EN71. According to the final
test on 16 kinds of the coloring agents, it is stipulated that a qualitative test and a semi-quantitative
determination method should first be performed by the photodiode array detection HPLC (HPLC-DAD).
Therefore, we examined this analytical method, and then improved its method.
By this improved method, we analyzed the 12 products (20 parts) of the textile products (toys and infant
clothes) that were commercially available. As a result, it was found that there were no products matching
to the reference products in both the spectrum and retention time, and that any of the regulated coloring
agents was not used in the products analyzed.
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