巻号一覧
11 巻 (1986)
- 4 号 p. 333-
- 3 号 p. 209-
- 2 号 p. 89-
- 1 号 p. 1-
11 巻, 4 号
選択された号の論文の6件中1～6を表示しています
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Gerard Rue1986 年 11 巻 4 号 p. 333-342
発行日: 1986年
公開日: 2008/04/18
ジャーナル フリーThe nucleus of the premeiotic oocyte from the hoplonemertine Amphiporus lactifloreus contains a characteristic DNA-rich body that rapidly generates a peculiar nucleolar apparatus previously described as the nucleolus-DNA body complex. During early vitellogenesis, the nucleolar complex is centrally located in the nucleus and consists of many spherical and ribbon-shaped components-the nucleolar spherulae and lamellae-which produce hundreds of small peripheral nucleoli and several granular bodies as vitellogenesis progresses. Distributions of the nucleolar organizer regions (NORs) in various nucleolar components were studied by silver staining, and results were compared with previous findings obtained by cytochemical and autoradiographic techniques.
The core of each spherula in the nucleolus-DNA body complex is strongly Ag-positive, and so, later on, is a minor part of each peripheral nucleolus. In contrast, the cortices of the nucleolar spherulae, in which slightly decondensed chromatin is distributed uniformly, and the nucleolar lamellae produced through self-peeling of the spherulae, do not react with silver. Thus, the NOR proteins identified by silver deposits correspond to numerous loci at which the most decondensed, transcriptionally active chromatin is made visible by the osmium-ammine reaction. These results are discussed in relation to the functional evolution of the initial DNA body of the oocyte from A. lactifloreus, the transcriptional abilities of its by-products, and the general process of the amplification of ribosomal genes.抄録全体を表示PDF形式でダウンロード (1427K) -
Rvoichi Hashida, Chie Anamizu, Yasuko Yagyu-Mizuno, Shoji Ohkuma, Tats ...1986 年 11 巻 4 号 p. 343-349
発行日: 1986年
公開日: 2008/04/18
ジャーナル フリーTranscellular transport of fluorescein dextran (FD) of various molecular weights (4K, 10K, 20K, 70K and 150K daltons) through porcine arterial endothelial cells cultured on a type I collagen gel supported by a dacron sheet was studied and compared with the transport of low density lipoprotein labeled with rhodamine B (RB-LDL) described previously (Hashida et al., Cell Struct. Funct. 11, 31-42, 1986).
The rate of FD transport through the monolayer depended on the size of the FD. FD transport was not temperature-dependent and was not a saturable process. Our findings show that FD transport differs from RB-LDL transport which is temperature-and dose-dependent. The mechanism of the transport of FD is compared with that of RB-LDL.抄録全体を表示PDF形式でダウンロード (627K) -
Matsuko Moriyasu, Akio Ito1986 年 11 巻 4 号 p. 351-358
発行日: 1986年
公開日: 2008/04/18
ジャーナル フリーThe procedure for immunochemical adsorption of vesicles with specific antigen on their outer surfaces was improved. When microsomal vesicles were mixed with Staphylococcus aureus cells coated with the antibody against NADPH-cytochrome c reductase, more than 90 % of the enzyme activity was adsorbed on the cell, whereas, only about 10 % of the activity was adsorbed on cells coated with the same amount of anti-ovalbumin antibody. NADH-cytochrome c reductase and aldehyde dehydrogenase activities were adsorbed on the cell to the same extent as was NADPH-cytochrome c reductase activity. Under this condition, there was no ad-sorption of the activities of the marker enzymes of lysosomes and Golgi ap-paratus, whereas large amounts of the activities of the plasma membrane enzymes were adsorbed. The specific activity of NADPH-cytochrome c reductase in the adsorbed vesicles from the microsomal fractions increased considerably. In contrast, marker enzymes of the Golgi or of the plasma membranes could be enriched in unadsorbed vesicles from the Golgi fractions.抄録全体を表示PDF形式でダウンロード (868K) -
Saburo Uchiyama, Shin-ichi Nagai, Keiichiro Yamaguchi1986 年 11 巻 4 号 p. 359-366
発行日: 1986年
公開日: 2008/04/18
ジャーナル フリーThe ultra-structure of the nucleolus in Dictyostelium dis-coideum cells was studied by electron microscopy. Large nucleoli on the peri-phery of the nucleus in cells of the multi-cellular pseudoplasmodium (slug) were maintained during long migration. Disaggregation of the slug cells induced a reduction in the size of the large nucleoli. The size of the reduced nucleoli in the reaggregated cells were maintained during the long migration and culmination of reconstructed slug. The electron density of the cytoplasm clearly distinguishes the prespore from the prestalk region, and it takes about 6 h for the complete recovery of cell-to-cell contact after reaggregation.抄録全体を表示PDF形式でダウンロード (1223K) -
Hiroya Kamei1986 年 11 巻 4 号 p. 367-377
発行日: 1986年
公開日: 2008/04/18
ジャーナル フリーOne hybridoma (AC54), which produces monoclonal antibody (MAb) that recognizes both intermediate filaments (IFs) and nuclear granules in BHK21/C13 cells, and two hybridomas (AC19 and AC36) which produce MAbs that recognize IFs only, were obtained by using a crude actin pre-paration from chicken gizzard as an antigen. In immunoblotting, both the AC54 and AC19 MAbs reacted with the 52 kD protein (desmin) and some other proteins in gizzard and BHK21/C13 cells. Indirect immunofluorescent microscopy of BHK21/C13 cells showed that the cytoplasmic filaments stained by these MAbs were IFs based on their colchicine-induced whorl formation. The ability of AC54 MAb to recognize IFs was more limited than that of AC19 MAb. The nuclear granules recognized by AC54 MAb were in a different location than the cytoplasmic IFs and sometimes were concentrated in the nucleolus.
These results indicate that AC54 MAb is an anti-desmin MAb that reacts with some desmin-related proteins; that it recognizes IFs differently than AC19 MAb, another anti-desmin MAb; and that it recognizes nuclear granules in locations where desmin or desmin-related protein has not yet been reported.抄録全体を表示PDF形式でダウンロード (4280K) -
A. J.Fernandez, M. A.Sierra, A. Mendez, E. Mozos, Ma. C.Moyano, L. Car ...1986 年 11 巻 4 号 p. 379-382
発行日: 1986年
公開日: 2008/04/18
ジャーナル フリーEffects of septic shock by repeated inoculations with Escherichia coli on the ultrastructure of the folliculo-stellate cells and cavities of the adenohypophysis of the chicken were investigated in order to determine the function of these cavities.
The principal morphological modifications were dilation of the Golgi apparatus, endoplasmic reticulum and autophagic vacuoles, and necrosis phenomena in the stellate cells. The follicular cavities showed dilation, and there was heterogeneous dense material and granular elements in the follicular lumen. Based on results reported in the literature, the observations reported here are evidence of a "cleaning-role", for the removal of cell debris, when there is endocrine disfunction.抄録全体を表示PDF形式でダウンロード (3316K)
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