巻号一覧
8 巻 (1983)
- 4 号 p. 315-
- 3 号 p. 199-
- 2 号 p. 91-
- 1 号 p. 1-
8 巻, 4 号
選択された号の論文の7件中1～7を表示しています
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Hou-Xiang Xie1983 年 8 巻 4 号 p. 315-325
発行日: 1983年
公開日: 2008/04/18
ジャーナル フリーThe efficiency of an exogenous gene's expression was com-pared after its transfection and injection into various mouse cells to system-atically evaluate these two gene transfer techniques. Special attention was paid to the period of transient expression. The gene used was a derivative of chicken δ-crystallin gene with the 5' end region replaced by a promoter base sequence of a retrovirus.
Nuclear injection was more efficient than transfection in several respects: it was roughly one thousand times more efficient in producing gene-expressing cells than the transfection technique; it produced positive cells in every challenged cell line in contrast to the results of some unsuccessful trials found with transfection; and the maximum expression of the exogenous gene in a gene-transferred cell was much higher after injection than after transfection. With the transfection technique, use of a DNA-calcium phosphate coprecipi-tate was slightly more efficient than the use of DEAE-dextran.
The stability of gene expression in transfected and nuclear-injected cells differed greatly: Expression of the exogenous gene in transfected cells was transmitted to 92% of the daughter cells per division, whereas its expression in injected cells was transmitted to only 32% of the daughter cells. This great difference in stability probably reflects different states of the major fraction of the exogenous gene: integration into chromosomes in transfected cells versus extrachromosomal localization in injected cells.抄録全体を表示PDF形式でダウンロード (1096K) -
Kenji Sorimachi1983 年 8 巻 4 号 p. 327-338
発行日: 1983年
公開日: 2008/04/18
ジャーナル フリーThe effect of concanavalin A on insulin binding was studied in cells cultured in a monolayer. Preliminary incubation of concanavalin A with Reuber rat hepatoma cells (R117-21B) showed an inhibitory effect on insulin binding at 25°C and 4°C; a "blocking" effect. But, even at high con-centrations (>50 μg/ml), concanavalin A could not completely inhibit the specific binding of insulin to these cells. Under these conditions, the number of high affinity insulin-binding sites decreased when compared to the number of sites in the control. In contrast, when cells first were incubated with insulin then with insulin plus concanavalin A, the binding of insulin increased, even at high concentrations of the lectin. This is a "trapping" effect. This effect increased the number of insulin-binding sites as compared to the number in the control. The apparent equilibrium constant of insulin for the cells was changed slightly by the concanavalin A in R117-21B cells.
The binding of concanavalin A to these cells also was investigated. Insulin did not affect its binding to the cells.
This study shows that insulin-binding sites can be classified into two groups in which insulin binding is affected by concanavalin A or is independent of the lectin. The "blocking" effect of concanavalin A on insulin binding suggests that the binding sites of insulin and concanavalin A on the insulin receptors are very close.抄録全体を表示PDF形式でダウンロード (1167K) -
Ikuo Yamaoka, Sumie Katsuta, Yoshimi Nagatani1983 年 8 巻 4 号 p. 339-346
発行日: 1983年
公開日: 2008/04/18
ジャーナル フリーLocalization of silver grains detected by the silver-impregna-tion method, a technique used to detect the classical Golgi apparatus, was examined with light and electron microscopy. Two types of silvered images of the Golgi apparatus were compared; each was obtained by Da Fano's silver-impregnation method, and one was modified with Caulfield's fixative during the preliminary fixation. Under ordinary light microscopy the images were very similar and showed the duplex structure of the Golgi apparatus which consists of an argentophil wall and argentophobe core. With electron microscopy, the relationship between the fine structure of the Golgi complex and the silver deposits was obtained in greater detail by the latter technique because the fine structure of the Golgi complex was retained. Many fine silver grains were detected in the cytoplasm adjacent to the Golgi complex, but none were present in the Golgi cisternae. This suggests that the argentophil wall of the duplex structure of the classical Golgi apparatus may be formed from argentophil substances that locate in the cytoplasm adjacent to the Golgi lamellae, and that the argentophobe core may be related to the Golgi lamellae.抄録全体を表示PDF形式でダウンロード (9357K) -
Koichiro Shiokawa, Yoshio Misumi1983 年 8 巻 4 号 p. 347-356
発行日: 1983年
公開日: 2008/04/18
ジャーナル フリーEffects of cordycepin on the incorporation of [3H] guanosine into embryonic Xenopus cells were examined. Cordycepin inhibited the labeling not only of poly(A)+RNA, but of all the the other major classes of RNAs. Cellular fractionation showed that this inhibition was much stronger in the labeling of cytoplasmic RNAs than of nuclear RNAs. [3H]Cordycepin was incorporated into both poly(A) +RNA and other RNA species.抄録全体を表示PDF形式でダウンロード (969K) -
T. Choku Takahashi, Hidemi Sato1983 年 8 巻 4 号 p. 357-365
発行日: 1983年
公開日: 2008/04/18
ジャーナル フリーThe sequence of mitosis in sea urchin eggs was investigated in the presence and absence of D2O. Direct observations of living cells under a polarizing microscope and observations with fixation-staining procedures were used. The duration of mitosis was extended by the presence of D2O. The slight extension of anaphase was due to elongation of the spindle in D2O, but the period from prophase to metaphase was clearly prolonged in the deuterated condition. These results indicate that D2O does not suppress anaphase chromosome movement, but does affect prometaphase and delays the alignment of chromosomes on the equatorial plane of the mitotic spindle at metaphase.
The stability of the isolated mitotic apparatus against Ca ions and low temperature also was investigated. There was no difference in the deterioration of isolated spindle birefringence under normal and deuterated conditions. The implications of these results are discussed in relation to the enhancement effect of D2O on the volume and birefringence of the living mitotic spindle.抄録全体を表示PDF形式でダウンロード (4060K) -
Hiroko Tsuda, W. Daryl Dickey, Armond S. Goldman1983 年 8 巻 4 号 p. 367-371
発行日: 1983年
公開日: 2008/04/18
ジャーナル フリーA new technique for separation of human colostral macrophages and neutrophils was developed. Macrophages attached more readily to acid soluble collagen-serum or gelatin-serum substrata than neutrophils. Most of the adherent neutrophils were removed during the first 5 minutes incubation in 3 mM EDTA, thereafter, complete detachment of macrophages followed. Neutrophils and macrophages were enriched more than 80% in nonadherent cell populations and detached cell populations, respectively. These separated colostral leukocytes retained their viability and phagocytic activities. Therefore, functional studies of these purified human colostral leukocytes are possible.抄録全体を表示PDF形式でダウンロード (453K) -
Kohzaburo Fujikawa-Yamamoto, Masami Wada1983 年 8 巻 4 号 p. 373-377
発行日: 1983年
公開日: 2008/04/18
ジャーナル フリーPhagocytosis of fluorescent microspheres (1.8 μm diameter) by Chinese hamster lung cells, V79 cells, was observed by flow cytometry and fluorescence microscopy. The phagocytic V79 cells observed by these methods had phagocytosis values that varied by less than 5 %.抄録全体を表示PDF形式でダウンロード (408K)
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