巻号一覧
19 巻 (1994)
- 6 号 p. 349-
- 5 号 p. 263-
- 4 号 p. 189-
- 3 号 p. 109-
- 2 号 p. 57-
- 1 号 p. 1-
19 巻, 2 号
選択された号の論文の7件中1～7を表示しています
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Hikoichi Sakai1994 年 19 巻 2 号 p. 57-62
発行日: 1994年
公開日: 2006/03/27
ジャーナル フリーPDF形式でダウンロード (1246K) -
Kazunori Ohki, Osamu Kohashi1994 年 19 巻 2 号 p. 63-71
発行日: 1994年
公開日: 2006/03/27
ジャーナル フリーWe examined the effect of laminin on DNA synthesis or cell growth of bone marrow-derived macrophages (BMM) and five macrophage cell lines including two factor-dependent macrophage cell lines (BDM-1 and BDM-1W3) and three factor-independent ones (BAM3, J774.1, and P388D1). Laminin stimulated the M-CSF-dependent proliferation of BMM and BDM-1cells in a dose-dependent manner, but it had no effect on the proliferation of BDM-1W3 cells. This stimulatory effect was modified by increasing the concentration of fetal calf serum in the culture medium. Laminin also stimulated the proliferation of BAM3 and J774.1 cells, whereas it could not stimulate that of P388D1 cells. Taken together, these results suggest that the differences in the response of these macrophage cell lines to the presence of laminin may be derived from the difference in the state of maturation of these cells. Although laminin acted as a potent mitogenic factor when it was added to the culture medium, the stimulation was two- to threefold lower when dishes were coated with equivalent amounts of laminin. When BDM-1 cells were cultured with laminin, they underwent drastic morphological changes. A monoclonal antibody that recognizes the integrin a6 subunit only partially inhibited the mitogenic effect of laminin. The results in this report suggest that laminin may participate in the regulation of macrophage populations invivo.抄録全体を表示PDF形式でダウンロード (2884K) -
Masaki Osawa1994 年 19 巻 2 号 p. 73-80
発行日: 1994年
公開日: 2006/03/27
ジャーナル フリーThe substance in sperm which induces the activation of eggs at fertilization has yet to be elucidated. Osawa et al. reported that the soluble extract of sperm (spex) causes inositol 1, 4, 5-trisphosphate (IP3)-induced Ca2+ release (IICR) in fertilized eggs of sea urchins when externally applied [OSAWA et al., (1992). Zool. Sci. 9 : 1206a]. This paper reports that spex also caused IICR in immature oocytes of Anthocidaris crassispina at their germinal vesicle stage and induced a transient increase in their intracellular Ca2+ concentration (Cai-transient). The peak value of this Cat-transient was 160±16 nM, a small increase by about 60 nM from the basal intracellular Ca2+ concentration, [Ca2+]i, of 96±12 nM. This reaction was completely blocked by heparin, an IICR inhibitor. Furthermore, the active factor in spex was resistant to pronase and did not show species specificity. It was found that Cai-transient can also be induced in oocytes by sperm. The peak value of the Cai-transient in this case was dependent on sperm concentration and was a maximum of approximately 250 nM, which was significantly lower than that at normal fertilization, i. e., 941 ± 136 nM. The Cai-transient induced by sperm in oocytes was not suppressed, though its time course was delayed, by heparin.抄録全体を表示PDF形式でダウンロード (1214K) -
Masaharu Kotani, Hiroshi Hosoya, Hideo Kubo, Kohji Itoh, Hitoshi Sakur ...1994 年 19 巻 2 号 p. 81-87
発行日: 1994年
公開日: 2006/04/19
ジャーナル フリーAlthough some intracellularly distributed glycosphingolipids are reported to be associated with vimentin intermediate filaments or colchicine sensitive cytoskeleton, no direct evidence for such an association has yet been shown. In this report we demonstrated that the intracellularly distributed ganglioside GM2 directly binds to isolated vimentin intermediate filaments in normal and Tay-Sachs disease human fibroblasts. Indirect immunofluorescence microscopy using a GM2-specific monoclonal antibody demonstrated filamentously distributed GM2 in the cytoplasm. A double staining of Tay-Sachs fibroblasts with anti-GM2 and anti-vimentin monoclonal antibodies strongly suggested that the GM2 positive filaments are vimentin intermediate filaments. We then isolated vimentin, in the presence of a detergent and urea, from the normal human skin fibroblasts and murine mastocytoma cells. In a solid phase enzyme-linked immunosorbent assay, the isolated vimentin dose-dependently reacted with both anti-vimentin and anti-GM2 monoclonal antibodies but not with anti-GM3 or anti-GM1 monoclonal antibody. The molar ratio of GM2 to vimentin was approximately 20 : 1. The lipid fraction extracted from the purified vimentin preparation was immunostained with anti-GM2 on a thin-layer chromatography plate. Furthermore, only one band was detected at the molecular weight of 57 kDa, after electroblotting and simultaneous immunostaining with anti-GM2 and anti-vimentin monoclonal antibodies. These results clearly indicated that ganglioside GM2 directly binds to vimentin.抄録全体を表示PDF形式でダウンロード (2331K) -
Taira Enomoto, Yuki Asano1994 年 19 巻 2 号 p. 89-96
発行日: 1994年
公開日: 2006/03/27
ジャーナル フリーTo investigate the biological characteristics of a Balb/c3T3 variant TR4 clone which is morphologically resistant to TPA and hypersensitive to v-src induced metastasis, we compared the responsiveness of the variant and its parent cells to growth factor-induced membrane ruffling. When the confluent cells were stimulated with PDGF, membrane ruffling was rapidly induced in TR4 but not in the parent cell cultures. In TR4 cells, membrane ruffling was observed under a phase-contrast microscope within 2 min after the addition of PDGF, reaching the maximum 5 min later and thereafter decreased gradually to the controllevel. There were no apparent differences in 125I-PDGF binding kinetics between TR4 and parent cells. Similar membrane ruffling was induced by other growth factors such as insulin, IGF-I, acidic or basic FGF but not by EGF or α- and β-TGF, only in TR4 cells. When TR4 cells were incubated with TPA just before stimulation with these growth factors, growth factor-induced membrane ruffling was completely inhibited. Also, 5 out of 6 clones of stable fusion cells between TR4 and parent cells showed the parental type of responses to TPA and growth factors, indicating that the TR4 phenotype is recessive. These results suggest that the variant TR4 cells may acquire the genetic and recessive alteration of a cellular factor which is responsible for the regulation of growth factor-mediated membrane ruffling and that this genetic alteration occurs at a common step downstream of growth factor-mediated cascades, rather than at their receptor level.抄録全体を表示PDF形式でダウンロード (3026K) -
Wolfgang Staiber1994 年 19 巻 2 号 p. 97-101
発行日: 1994年
公開日: 2006/03/27
ジャーナル フリーThe changes in microtubule arrangements correlated with the behavior of the germ line limited and soma chromosomes were studied during the last unequal gonial mitosis, the so-called differential mitosis, of the chironomid Acricotopus lucidus by indirect immunofluorescence using a monoclonal anti-β-tubulin antibody, and by simultaneous staining with the DNA-specific fluorescence dye DAPI. An impressive difference in microtubule density between both half spindles was determined in metaphase and during the monopolar anaphasic migration of the germ line limited chromosomes. In the following normal separation of the soma chromosomes, a similar microtubule density in both half spindles occurred. In each of the half spindles, chromosome movement and spindle elongation occurred independently, and in one half spindle two anaphasic chromosome movements ran off one after another, the second without a simultaneous spindle elongation.抄録全体を表示PDF形式でダウンロード (2912K) -
Generoso Manoel Chagas, Maria Lúcia Wambier Klüppel, Annib ...1994 年 19 巻 2 号 p. 103-108
発行日: 1994年
公開日: 2006/03/27
ジャーナル フリーThe cytotoxicity of citrinin was evaluated in an established cell line of baby hamster kidney cells. The primary effect of the mycotoxin was on the adherence of the cells to the culture bottles. Microscopic evaluation of morphological alterations indicated that the cells which were originally elongated and flattened, became swollen and round. Electron microscopic examination showed that citrinin (0.1, 0.5 and 1.0mM) incubated for 10 hours with cultured cells, promoted drastic alterations of normal mitochondria, with swelling and cell death. Transplasma membrane redox system is inhibited by citrinin (81%). This effect is dependent not only on the toxin concentration, but also on the time of exposure to the cells.抄録全体を表示PDF形式でダウンロード (4997K)
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