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Online ISSN : 2424-0664
Print ISSN : 0916-6920
ISSN-L : 2424-0664
16 巻 , 2 号
選択された号の論文の7件中1~7を表示しています
第15回日本サイトメトリー学会学術集会優秀講演賞
  • 渡辺 信和, 高橋 聡, 三鶴 亜矢子, 高橋 圭介, 大井 淳, 友成 章, 小沼 貴晶, 森 毅彦, 岡本 真一郎, 長村-井上 登紀子 ...
    2006 年 16 巻 2 号 p. 1-9
    発行日: 2006/09/25
    公開日: 2017/07/04
    ジャーナル フリー

    Cord blood transplantation (CBT) frequently can be complicated by cytomegalovirus (CMV) infection, which is required for administration of antiviral chemotherapy. Although the naïve feature of cord blood T-cells were suggested to increase the incidence of CMV infection, the underlying mechanism is not fully elucidated. CMVresponsive T-cells detected by intracellular interferon-gamma (IFN-γ) staining and CMV-specific CD8+ T- cells detected by MHC class I tetramers were analyzed in healthy adult blood and recipient blood post-CBT. CMV antigenemia was accompanied in thirty-two of thirty-nine recipients (82%) post-CBT. CMV-responsive CD4+ T-cells were detected in all recipients with CMV antigenemia within 120 days post CBT [first date of CMV- responsive CD4+ T-cell detection, median 41 days, (32-90 days); peak frequencies 18±21 cells/μl, n=22]. In contrast, CMV-responsive CD8+ T-cells were detected in only 38% of patients with CMV antigenemia with similar frequencies (peak frequencies 18±24 cells/μl, n=8). CMV-responsive CD4+ T-cells but not CMV responsive CD8+ T-cells were significantly induced in all recipients with CMV antigenemia. Rapid reconstitution of CMV-responsive CD4+ T-cells might suppress the progression of CMV disease from CMV antigenemia.

原著
  • 塩沢 裕介, 北村 紀子, 竹野内 寿美, 田口 智子, 大喜多 肇, 林 泰秀, 小原 明, 花田 良二, 土田 昌宏, 藤本 純一郎, ...
    2006 年 16 巻 2 号 p. 11-17
    発行日: 2006/09/25
    公開日: 2017/07/04
    ジャーナル フリー

    Aim. We are in charge of the central diagnosis and cell preservation as a part of childhood acute lymphoblastic leukemia treatment study in Tokyo Children’s Cancer Study Group. It is necessary to diagnose with a minimal quantity of specimen, to preserve leukemic cells effectively as possible. Therefore a diagnosis of childhood acute lymphoblastic leukemia by four-color analysis with digital flow cytometer has been examined.

    Methods. We examined cell markers of childhood acute lymphoblastic leukemia cells by four-color analysis using digital flow cytometers. We selected the monoclonal antibodies for the diagnosis based on the recommendation of Japan Pediatric Lymphoma Study Group and made out a panel of antibodies which enable us to confirm aberrant antigen-expressions on the leukemic cells.

    Results. Four colors that we used in this study were fluorescein isothyocyanate, phycoerythrin, phycoerythrin-cyanin 5.1, and phycoerythrin-cyanin-7. The most of childhood acute lymphoblastic leukemia cases could be diagnosed without CD45-gating. List mode compensation was useful to re-investigate specimens which was difficult to re-examine, because there were very few.

    Discussion. Four-color analysis using digital flow cytometer is useful to save precious specimen of childhood acute lymphoblastic leukemia. We are intending to perform five-color analysis with CD45-gating as a next step.

  • 中井 英勝, 渡部 洋, 上田 晴彦, 星合 昊
    2006 年 16 巻 2 号 p. 19-23
    発行日: 2006/09/25
    公開日: 2017/07/04
    ジャーナル フリー

    Relative DNA content of tumor cells determined by flowcytometric analysis (DNA ploidy) has been reported as a prognostic factor of solid cancer. However, it is still unknown correlation between morphologic characteristics and DNA ploidy. We have evaluated the cytopathologic findings including area of nucleus, area of nucleolus, number of nucleolus, nuclear/ cytoplasm ratio, and DNA ploidy in 76 patients with uterine endometrial carcinomas. DNA aneuploid was detected in 18 patients (24%), and DNA aneuploid cases exhibited significantly larger nuclear area and area of nucleolus than that of the DNA diploid cases. However, no correlation was seen in nuclear/cytoplasm ratio, and number of nucleolus. Our findings suggest that relative DNA content would be influenced to area of the nucleus and area of the nucleolus.

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