The species definition of
Spherillo obscurus (Budde-Lund, 1885) was redefined based on observations of syntype and specimens collected from a broad-leaved forest in The University of Tokyo (Hongo Campus), Japan. The species definition of
S. dorsalis (Iwamoto, 1943) was also modified based on the specimens collected from a broad-leaved forest in Yokohama National University, Japan. Partial DNA sequences of mitochondrial cytochrome oxidase subunit I and 12S and 16S ribosomal RNA and of nuclear 18S and 28S ribosomal RNA were determined for identifying these species.
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