It is advantageous to use adhesive root canal sealer for prevention of reinfection in pulpectomy. Mineral trioxide aggregate (MTA) is a hydraulic cement with bactericidal action and hard tissue induction ability and it has been widely used in dental treatment. The purpose of this study was to examine the effect of cell growth on adhesive root canal sealer containing white portland cement (WPC) for development of new adhesive root canal sealer with efficacy of MTA. There was no change in the number of cells in which human periodontal ligament cells survived in the culture medium containing components eluted from the experimental sealer in case of low concentration. The pH of the culture medium and the concentration of Calcium dissolved in the culture medium were WPC concentration-dependent increase. More research is required to determine if MTA-containing adhesive root canal sealer will induce hard tissue at root apex.