Abstract : We investigated the effects of power and time of 810-nm Ga-Al-As laser irradiation on the calcification ability of human dental pulp (HDP) cells.
To investigate the effects of laser power, cultured HDP cells underwent single 810-nm Ga-Al-As laser irradiation at 300 J with total irradiation energy fixed at 0 W/0 s (control group), 0.5 W, 1.0 W, 1.5 W, 2.0 W, 2.5 W or 3.0 W. von Kossa staining, alkaline phosphatase (ALP) activity, and cell number of the HDP cells were determined to evaluate the calcified nodule formation. von Kossa staining was greater in the 1.0 W group or 1.5 W group than in the control group, as were cell number and ALP activity (p<0.05). However, von Kossa staining was lower in the 3.0 W group than in the control group, as were cell number and ALP activity (p<0.05).
To investigate the effects of laser irradiation time on the calcification ability, HDP cells were irradiated once with the laser at 1.0 W for 0 s (control group), 10 s, 30 s, 50 s, 100 s, 300 s or 500 s. Cell number and ALP activity were enhanced by laser irradiation in a time-dependent manner, and were significantly greater in the 500 s and 300 s groups compared with the control group (p<0.05). Calcified nodule formation in the HDP cells increased in a time-dependent manner after laser irradiation, as revealed by von Kossa staining.
These results suggested that formation of calcified nodules in HDP cells was enhanced by stimulation under optimum laser irradiation conditions.
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