Purpose: In this study, the authors histomorphologically investigated the usefulness of porous β-tricalcium phosphate (β-TCP) block with two different pore rates as a carrier of bone formation factor, recombinant human bone morphogenetic protein-2 (rhBMP-2), and bone formation.
Methods: Using 10-week-old female SD rats, the parietal region was incised, and the periosteum was exfoliated to expose the parietal bone. The following four conditions were established: 1.β-TCP alone with a pore rate of 60%(60 TCP); 2. rhBMP-2 was immersed in β-TCP with a pore rate of 60%(60 TCP+BMP); 3.β-TCP alone with a pore rate of 75%(75 TCP); and 4. rhBMP-2 was immersed in β-TCP with a pore rate of 75%(75 TCP+BMP). In the 60 TCP+BMP and 75 TCP+BMP groups, 0.2 mg/cm
3 β-TCP was added to rhBMP-2. The follow-up period was established at 2 and 4 weeks. After the animals were sacrificed, nondecalcified polished specimens were prepared for histomorphological investigation.
Results: In the 60 TCP and 75 TCP groups, no neogenetic bone formation was detected. In the 60 TCP+BMP and 75 TCP+BMP groups, neogenetic bone formation was observed. Furthermore, β-TCP with a pore rate of 75% showed more rapid absorption.
Conclusions: In the 60 TCP and 75 TCP groups, neogenetic bone formation was observed, suggesting that a complex can be prepared and that β-TCP block facilitates maintaining and gradually releasing rhBMP-2, thus β-TCP block may be a useful carrier. Therefore, treatment with β-TCP block may improve bone defects.
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