We report the case of a 30-year-old pregnant woman with uterine cervical cancer, in which the tumor contained both small cell carcinoma and tubular adenocarcinoma components. Cytology showed atypical cells with small bare nuclei, scattered singly or in loose clusters, in addition to atypical glandular tissue. Cervical biopsy demonstrated both small cell carcinoma and tubular adenocarcinoma components. On immunohistochemistry, the small cell carcinoma cells showed positive staining for chromogranin A, somatostatin, NSE and SSTR2. At 29 weeks of gestation, the patient underwent abdominal cesarean section, delivering a female baby, followed by radical hysterectomy ; thereafter, she received chemotherapy. Until now, there has been no evidence of recurrence.
We examined the usefulness of ultra-rapid immunostaining of ascitic fluid for HNF4α, which is expressed in abundance in the gastrointestinal tract epithelium, for rapid and precise intraoperative cytological diagnosis of gastric cancer. In this examination, we used the R-IHC® system to obtain rapid results, and the results revealed that HNF4α was specifically expressed in gastric cancer cells and that no positively stained cells were found in other malignancies such as lung cancer, or in benign conditions. Therefore, ultra-rapid HNF4α immunostaining of ascitic fluid using R-IHC® was useful for rapid intraoperative cytological diagnosis of gastric cancer.