Journal of the Japanese Society for Horticultural Science
Online ISSN : 1882-336X
Print ISSN : 1882-3351
ISSN-L : 1882-3351
Volume 80, Issue 3
Displaying 1-16 of 16 articles from this issue
REVIEW
  • Miyuki Kunihisa
    Article type: Review
    2011 Volume 80 Issue 3 Pages 231-243
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    The cultivated strawberry, Fragaria × ananassa (2n = 8x = 56), is an economically important crop in many regions of the world, and many breeding programs are competing to produce new, high-value cultivars. Despite this level of interest, marker-assisted selection of strawberry based on QTL analysis has lagged behind that of diploid crops because of its complex polyploidy and unclear genomic construction. During the past decade, great progress in the development of DNA markers has begun to overcome these difficulties, and the study of inheritance, mapping, and phylogeny is proceeding rapidly. Meanwhile, strawberry breeders have become much more aware of their rights with respect to the cultivars they have developed, as the volumes of harvested fruits that are transported globally have increased. To address the need to identify illegally grown strawberry fruits, genomics researchers have been required to develop simple and reproducible techniques for cultivar identification. This review summarizes the recent development of DNA marker studies in F. × ananassa, with a focus on the genome-specific markers we have developed.
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ORIGINAL ARTICLES
  • Jijun Li, Sadao Komori, Ken Sasaki, Naozumi Mimida, Shogo Matsumoto, M ...
    2011 Volume 80 Issue 3 Pages 244-254
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    Transformation experiments in apple (Malus × domestica Borkh.) were conducted to improve the Agrobacterium-mediated transformation efficiency of many apple cultivars without using complicated procedures. A system of direct regeneration from leaf segments was used for these experiments. The effectiveness of meropenem hydrate (MEPM) as an antibiotic for Agrobacterium elimination was investigated for use of instead of cefotaxime sodium (CTX), which prevents shoot regeneration from apple leaf segments. Results show that 50 mg·L−1 of MEPM greatly enhanced the transformation efficiency. Experiments assessing the timing of kanamycin sulfate (KM) addition to the medium were also conducted to investigate the effective use of KM, the antibiotic used to select transformed cell. Addition of KM to the medium immediately after co-cultivation was completed is advantageous for the multiplication of transformed cells and for the induction of transformed shoots in spite of the low shoot regeneration rate. Based on knowledge of the timing of KM addition, the effects of pre-culture before Agrobacterium infection on the shoot regeneration medium were investigated. Pre-culture for 3–7 days improved the transformation efficiency. By applying both MEPM and the pre-culture, about 5% transformation efficiency in ‘Greensleeves’ was achieved on an apple cultivar without complicated procedures.
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  • Hironori Kobayashi, Shunji Suzuki, Tsutomu Takayanagi
    2011 Volume 80 Issue 3 Pages 255-267
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    The aim of this study was to elucidate the effects of climatic conditions in three grape-growing regions on ‘Koshu’ (Vitis vinifera) grape composition over a four-year period from 2004 to 2007. Total solar radiation was the highest in Kofu and the total soluble solids (TSS) concentration of grapes grown in this region was higher than those of grapes grown in the other two regions. Katsunuma showed a large difference between average maximum and minimum air temperatures, and anthocyanin concentration in grape skin was the highest. In addition, grape juice had high total phenolics (TP) concentration. Nirasaki had the highest elevation of the three regions tested. Because of this, the maturity of grape berries grown in the Nirasaki vineyard was delayed by a couple of weeks compared to that of grape berries grown in Kofu vineyard. Yeast assimilable nitrogen (YAN) content in grape juice might be not affected by climatic conditions in each growing region. A strong correlation was noted between total solar radiation in August (10 to 13 weeks post-flowering; WPF) and ‘Koshu’ grape composition compared with other climatic conditions, such as rainfall, average air temperature, and the difference between maximum and minimum air temperatures. Moreover, the correlation coefficients between average air temperature and the grape composition in August were also higher than those 14 to 17 WPF (September) and 18 to 20 WPF (October). Thus, climatic conditions in August influenced ‘Koshu’ grape composition. These findings are expected to greatly contribute to the selection of suitable cultivation regions for ‘Koshu’ grape and the improvement of ‘Koshu’ wine quality.
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  • Masashi Yamamoto, Rumiko Kouno, Tsuyoshi Nakagawa, Takuma Usui, Tatsuy ...
    2011 Volume 80 Issue 3 Pages 268-275
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    Genetic relationships among local Citrus accessions on the Amami islands, Japan, were evaluated using isozyme, random amplified polymorphic DNA (RAPD) and cleaved amplified polymorphic sequence (CAPS) of chloroplast DNA (cpDNA) analyses. Four loci were detected for three enzymes examined in isozyme analysis. Four and three kinds of genotypes were detected in glutamate oxaloacetate transminase (GOT)-1 and GOT-2, respectively. At least six genotypes were observed in peroxidase (PX). All accessions showed the same genotype except for one accession of superoxide dismutase (SOD)-3. In general, accessions that belong to the same species or type showed identical genotypes; however, some diversity of genotypes was observed within the same species and type. On the basis of the RAPD data, genetic relationships were assessed using NJ cluster analysis. From this result, accessions could be classified into three major clusters, A, B, and C. Cluster A included C. nobilis (Kunenbo), C. keraji (‘Keraji-Kikaijima’ and Kabuchii), C. oto (Oto), ‘Keraji-Kakeromajima’, and ‘Oto-Okinoerabujima’. Cluster B included only control accessions. Cluster C could be divided into four subclusters as follows: subcluster C. depressa (Shiikuwasha), some Sour orange relatives such as ‘Fusuu’ and ‘Kusa’, ‘Kikaimikan-Okinoerabujima’, and ‘Shimamikan’. Accessions were classified into three types in cpDNA analysis. Type I included C. nobilis (Kunenbo), C. keraji (‘Keraji-Kikaijima’ and Kabuchii), C. oto (Oto), ‘Keraji-Kakeromajima’, ‘Oto-Okinoerabujima’, and C. rokugatsu. Type II was composed of C. depressa (Shiikuwasha), ‘Kikaimikan-Okinoerabujima’, ‘Kusa’, and ‘Shiikuu’. Type III consisted of only ‘Shimamikan’. All mandarin accessions that were determined to belong to cluster A in RAPD analysis were included in type I of cpDNA analysis. Meanwhile, mandarins in types II and III in cpDNA analysis consisted of accessions in cluster C of RAPD analysis.
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  • Hisayo Yamane, Ryutaro Tao, Tomomi Ooka, Hiroaki Jotatsu, Ryuta Sasaki ...
    2011 Volume 80 Issue 3 Pages 276-283
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    This study investigated the regulation of the seasonal expression of PpDAM5 and PpDAM6, two of the six peach (Prunus persica) dormancy-associated MADS-box genes, in relation to the endodormancy and development of lateral vegetative and flower buds of low- and high-chill peach cultivars. PpDAM5 and PpDAM6 were originally found as homologs of Arabidopsis SVP/AGL24 at the EVERGROWING (EVG) locus of peach and have been recently shown to be involved in lateral bud endodormancy. Seasonal expression analyses in this study indicated that PpDAM5 and PpDAM6 transcript levels in lateral vegetative buds of both low- and high-chill cultivars in the field negatively correlated with bud burst percentages determined under forcing conditions. Negative correlation was also found between their transcript levels and the flower organ enlargement rate. These results suggest that distinct seasonal expression patterns of PpDAM5 and PpDAM6 are correlated with a distinct chilling requirement for bud break and flowering of low- and high-chill cultivars. Characterization of the genomic structure of PpDAM5 and PpDAM6 revealed the presence of large insertions in the first introns of both PpDAM5 and PpDAM6 in low-chill peach. Alteration of the genomic structure is discussed with respect to the low-chill character.
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  • Binh Xuan Ngo, Jung-Hee Kim, Akira Wakana, Shiro Isshiki, Tomoyo Mori
    2011 Volume 80 Issue 3 Pages 284-294
    Published: 2011
    Released on J-STAGE: July 22, 2011
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    Self-incompatibility (SI) in Citrus is known in cultivars and plants of pummelo, pummelo relative, and mandarin; however, the detailed mechanism of self-incompatibility and the allelic diversity of the SI gene (S) are not known because of the presence of barriers such as nucellar embryony, male sterility, and a long juvenile phase. Thus, there is little information on S genotypes of Citrus cultivars at present. In this study, the S genotypes of Citrus cultivars were estimated with the aid of allozymes produced by a glutamate oxaloacetate transaminase isozyme gene (Got-3), which appeared to link to the S gene. Of twenty-two F1 progenies from eleven crosses and their reciprocal crosses with eight monoembryonic SI cultivars, including ‘Banpeiyu’ pummelo, three F1 progenies from three crosses and three from reciprocal crosses showed segregation distortion for the Got-3 gene, suggesting the presence of the same S alleles in the cultivars used for the reciprocal crosses. Defining the ‘Banpeiyu’ genotype as S1S2, the genotype was estimated as S1S3 for ‘Tosa Buntan’, S4S5 for ‘Hassaku’, S6S7 for ‘Yuge-hyokan’, and S1S6 for ‘Shishiyuzu’. Further allozyme analyses for segregation distortion in progenies from eight crosses with eight monoembryonic SI cultivars suggested the possibility that ‘Hyuganatsu’ has S1 allele, while those from 15 crosses between the eight monoembryonic SI cultivars and five polyembryonic self-compatible cultivars suggested the possibility that ‘Rough lemon’ has S1 allele. The estimated genotypes, consisting of two of the nine alleles (S1 to S8 and Sf) in these Citrus cultivars, may be useful for further estimation and determination of S genotypes in Citrus cultivars and plants.
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  • Satoshi Ohta, Tomoko Endo, Takehiko Shimada, Hiroshi Fujii, Tokuro Shi ...
    2011 Volume 80 Issue 3 Pages 295-307
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    Citrus tristeza virus (CTV) is an acute pathogen that causes serious damage to the citrus industry. Poncirus trifoliata (L.) Raf., a sexually compatible species with Citrus, has resistance against a broad range of CTV strains. Breeding programs have been conducted to introduce the CTV resistance gene from P. trifoliata to Citrus, but no commercial cultivar has yet been developed. In this study, we developed four selection markers linked to CTV resistance to enable marker assisted selection to efficiently introduce CTV resistance into Citrus. The four CTV resistance-linked markers were composed of a co-dominant single nucleotide polymorphism (SNP) marker and three dominant sequence tagged site (STS) markers. All four markers were fitted to the progeny and were linked to CTV resistance, with only 2.8% exceptions. We also developed 46 P. trifoliata allele identification markers from alleles of 35 Citrus species. Among the 46 markers, nine were located in linkage group 2, on which the CTV resistance locus is located. We located the other 31 markers on the rest of the linkage groups so that these markers could be used to distinguish P. trifoliata genome regions remaining in the hybrid progenies. The set of PCR primers developed in this study will be useful for marker assisted backcrossing to introduce the P. trifoliata CTV resistance gene into Citrus.
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  • Kazuna Horiuchi, Yumiko Adachi, Noboru Kasai, Masumi Yamagishi, Kiyosh ...
    2011 Volume 80 Issue 3 Pages 308-313
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    Discrimination of homologous and heterozygous plants at the male-determination locus in asparagus (Asparagus officinalis L.) was examined by quantitative analyses of a diagnostic marker for male asparagus. The quantitative difference of a sequence, AspTaq1, derived from the Asp1-T7 diagnostic marker (Jamsari et al., 2004), was measured using the real-time monitoring PCR and normalized based on the quantitative difference of a sequence, AODEF-Taq1, derived from a single copy gene, AODEF. The normalized difference was higher in established supermales than in regular males grown from commercially available seeds. The averaged values in respective groups are in the approximate ratio of 2 : 1. Homozygous male (MM), heterozygous male (Mm), and female (mm) genotypes were obtained by selfing of an andromonoecious line. The progeny were divided into three groups by the normalized difference; plants showing high values and intermediate values were thought to be homozygous and heterozygous males, respectively. Significant amplification of AspTaq1 was not detected in several plants; therefore, they were judged to be females. Then, to identify supermales, pollen from male plants was crossed separately with female plants, and the sex of the next generation was determined. Pollen from two male plants showing high values of the normalized difference generated only males in the next generation, while pollen from 12 male plants showing intermediate values generated both female and male plants. Pollen from one male plants that showed intermediate values generated only male plants. The normalized difference in homozygous males was comparable to the value obtained using a recombinant sequence that contains an equal number of AspTaq1 and AODEF-Taq1. These results indicate that homozygous and heterozygous males can be identified by quantitative analysis of a nucleotide sequence linked to the sex-determination locus and that the use of an authentic sequence for calibration facilitates the discrimination process of those genotypes in asparagus plants.
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  • Noor Elahi Jan, Saneyuki Kawabata
    2011 Volume 80 Issue 3 Pages 314-321
    Published: 2011
    Released on J-STAGE: July 22, 2011
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    Tomato fruits accumulate most of their solid contents during rapid fruit development. The balance of water transport to assimilate transport into fruits during that stage is an important factor that determines the final solid content of fruits at harvest. In this study, the relationship of sucrose concentration of the phloem sap to the solid content of rapidly growing fruits was investigated. At the anthesis of the second truss, the stem just above the first truss was heat-girdled, while newly emerging leaves above the second truss were pinched regularly to diminish cross translocation of carbohydrates between the source-sink unit, consisting of the second truss and three leaves below, and other parts of the plant. The leaf to fruit ratio (LFR) as the number of leaves per fruit of this isolated source-sink unit was manipulated at the fruit set of the truss. When LFR was increased from 0.2 to 1, fruit dry weight increased linearly from 3.5 to 7 g, but it saturated beyond the LFR of 1. By contrast, the contents of dry matter and soluble sugars on a fresh weight basis increased linearly within the whole range of LFR from 0.2 to 3. The sucrose concentration of the phloem sap, collected from the pedicel by the modified EDTA method, also increased as LFR increased, and a positive correlation was observed between sucrose concentration of the phloem sap and the contents of dry matter and total soluble sugars. These data suggested that solid content can be increased in a wide range of LFR in proportion to the sucrose concentration of the phloem sap.
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  • Noriya Masamura, Shigenori Yaguchi, Yasunori Ono, Tetsuya Nakajima, Sh ...
    2011 Volume 80 Issue 3 Pages 322-333
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    Eight Japanese bunching onion (Allium fistulosum L.)—shallot (Allium cepa L. Aggregatum group) monosomic addition lines (MALs, FF + 1A–FF + 8A) were used to reveal the effects of single alien chromosomes of A. cepa on the production of amino acids and S-alk(en)yl-L-cysteine sulfoxides (ACSOs) in leaf tissues of A. fistulosum. Amino acid and ACSO contents in MAL leaf blades were determined once every 3 months from August 2005 to May 2006. The amino acid found in the greatest amount in all of the MALs throughout the year was cysteine, except for FF + 7A accumulated glycine as the greatest amount of amino acid (Nov. 2005). All of the MALs contained three ACSOs in varying amounts and proportions, and all accumulated S-1-propenyl CSO as a major ACSO but hardly produced S-2-propenyl CSO throughout the year. FF + 3A showed greatly increased proportions of S-methyl CSO in total ACSO, suggesting that anonymous genes controlling S-methyl CSO production are located on chromosome 3A of shallot. High accumulation of total ACSOs in the monosomic additions FF + 3A, FF + 4A, FF + 5A, and FF + 8A was observed during different growth periods. Using PCR-based marker analysis, sulfate transporter, adenosine 5'-phosphosulfate reductase (APSR), serine acetyltransferase, O-acetylserine thiol-lyase, glutamylcysteine synthase, glutathione synthase, and γ-glutamyl transpeptidase candidate genes related to sulfur assimilation and ACSO biosynthesis were allocated to chromosomes 7A, 2A, 7A, 7A, 2A, 7A, and 4A, respectively. This result showed little association between the chromosomal locations of these genes and ACSO accumulation, suggesting that anonymous genes controlling ACSO accumulation were dispersed on the 2A, 3A, 4A, 5A, 7A, and 8A chromosomes of shallot. APSR gene expression was inhibited by 5A chromosome additions, suggesting that one of the regulatory genes was located on a 5A chromosome and inhibited APSR expression.
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  • Takaaki Nishijima, Tomoya Niki, Tomoko Niki
    2011 Volume 80 Issue 3 Pages 334-342
    Published: 2011
    Released on J-STAGE: July 22, 2011
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    We previously reported that cytokinin (CK) application enlarged the corolla of petunia (Petunia hybrida Vilm.). In this paper, the effect of the large-flowered trait of petunia caused by the Grandiflora (G) allele to CK biosynthesis was investigated. Concentrations of endogenous CK nucleobases (i.e., N6-(Δ2-isopentenyl)adenine (iP) and trans-Zeatin (tZ)), CK nucleosides (iP riboside and tZ riboside), and a CK glucoside (iP-7-glucoside) in the developing corolla were markedly lower in large-flowered cultivars with a Gg genotype than small- and medium-flowered cultivars with a gg genotype. We isolated the cDNAs encoding CK biosynthesis enzymes expressed in the young corolla, i.e., cDNAs of an adenosine phosphate-isopentenyltransferase gene (SHO), a CK riboside 5'-monophosphate phosphoribohydrolase gene (PhLOG), and two CK oxidase/dehydrogenase genes (PhCKX1 and PhCKX2). Expression levels of SHO and PhLOG in the developing corolla were not markedly different among small-, medium-, and large-flowered cultivars. However, expression levels of PhCKX1 and PhCKX2, which catalyze CK degradation through removal of the isoprenoid side chain, were much higher in large-flowered cultivars than in small- and medium-flowered cultivars. Therefore, low CK concentrations in large-flowered cultivars are very probably induced by the promoted expressions of PhCKX1 and PhCKX2, which may partially repress corolla enlargement.
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  • Takaaki Nishijima, Tomoya Niki, Tomoko Niki
    2011 Volume 80 Issue 3 Pages 343-350
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    Expressions of the genes involved in the cytokinin (CK) phosphorelay signaling circuitry in petunia (Petunia hybrida Vilm.) were investigated with regard to the large-flowered trait induced by the semidominant Grandiflora (G) gene. We isolated three cDNAs encoding type-A response regulators (RRs, PhRR1-3) and one cDNA encoding CK receptor histidine protein kinase (PhHK). Expression levels of PhRR1-3 and PhHK in the developing corolla were markedly higher in large-flowered cultivars with the Gg genotype than in small- and medium-flowered cultivars with the gg genotype. Higher expression levels of PhRR1-3 and PhHK in the Gg than in the gg genotypes was also consistent in BC4 plants in which the genetic background except for G allele was mostly uniform. These results suggest that Gg genotype induces a high expression level of PhRR1-3 and PhHK. These changes in expression caused by the G allele were the same as those caused by the CK response. Since type-A RRs repress CK signaling, the G-allele-induced high expression levels of PhRR1-3 presumably have partial inhibitory effects on G-allele-induced corolla enlargement.
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  • Qiuhong Wang, Yang Zhang, Saneyuki Kawabata, Yuhua Li
    2011 Volume 80 Issue 3 Pages 351-357
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    The double fertilization process and the development of endosperm and embryo were observed by microscopy in Eustoma grandiflorum. The flowers were fixed at various times after hand-pollination during stages of pollen tube extension, entry of pollen tubes into ovule, double fertilization, and early development of the embryo. Pollen grains initiated germination and penetrated the style 4 hours after pollination (HAP). Pollen tubes grew toward the ovule through the intercellular spaces along the solid style at 8 HAP. During pollen tube extension at 24 HAP, the generative cell divided into two sperm cells. Pollen tubes reached the ovary at 48 HAP, extended directly into the degenerating synergid cell and released the two sperm cells. One of the two sperm cells arrived adjacent to the egg cell at 60 HAP. Four to 5 days after pollination, a zygote was formed. The sperm nucleus adhered to the nuclear membrane of the secondary nucleus at 60 HAP. The primary endosperm nucleus was formed at 72 HAP. The zygote underwent the first mitosis 20 days after pollination. After the first divisions of the zygote, the apical cell divided transversely initially, and continued to develop to form the embryo proper; hence, the embryogenesis was of Solanad type. The mature seeds have a heart-shaped embryo. Only one spindle formed during the first mitosis of the zygote, and the fusion of male and female nucleoli took place before mitosis. The karyogamy was therefore of premitotic type.
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  • Shigeto Morita, Yuka Torii, Taro Harada, Masaya Kawarada, Reiko Onoder ...
    2011 Volume 80 Issue 3 Pages 358-364
    Published: 2011
    Released on J-STAGE: July 22, 2011
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    Flower opening in carnations (Dianthus caryophyllus L.) is the result of the enlargement of petal cells, which requires sugar metabolism. A cDNA encoding sucrose synthase (DcSUS1) was isolated from carnation petals as a candidate gene acting in the initial step of sugar metabolism in petal cells. DcSUS1 transcripts were detected abundantly in floral tissues of flowering carnation plants; the transcripts accumulated most in the petals and style followed by the ovary, whereas only small accumulation were found in stems, leaves, and calyces. Moreover, nearly constant accumulation of DcSUS1 transcripts was found in the petals during flower opening, fully open, and early senescence periods, whereas decreasing accumulation was detected in petals when senescence progressed. These findings suggested the involvement of DcSUS1 expression in petal cell growth during the opening of carnation flowers.
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  • Jingtair Siriphanich, Anak Pakcharoen, Kiranun Mohpraman, Rujira Tisar ...
    2011 Volume 80 Issue 3 Pages 365-371
    Published: 2011
    Released on J-STAGE: July 22, 2011
    JOURNAL OPEN ACCESS
    Uneven fruit ripening is often observed in durians (Durio zibethinus Murray), but its cause is unknown. In order to understand the development of this disorder, ‘Mon-Thong’ durians from leaf flushing and shaded trees were studied. It was found that durians normally accumulated photosynthate and ripened, by converting starch to sugar, unevenly in the direction from the stylar end to the stem end; however, in terms of softening, uneven ripening was found to be non-directional. The fruit from leaf flushing trees was found to have a higher uneven fruit softening score, as well as higher variation in pulp firmness but not soluble solids content, than those harvested from control trees. In addition, fruit from 50% shaded trees, 99–105 days after full bloom (DAF), was compared to fruit from non-shaded trees. Higher uneven fruit softening was found in durians from shaded trees. When durians were harvested again one week after removal of shading, they had similar ripening characteristics to those from control trees. It was shown that factors that limit fruit development and its maturity positively enhance the non-directional softening pattern. It was also found that treating durians with a high concentration of ethephon could not alleviate this phenomenon.
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  • Eric Gituma Mworia, Takashi Yoshikawa, Nadiah Salikon, Chisato Oda, Te ...
    2011 Volume 80 Issue 3 Pages 372-377
    Published: 2011
    Released on J-STAGE: July 22, 2011
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    The effects of modified atmosphere (MA) storage and application of 1-methylcyclopropene (1-MCP) at harvest on the storability and quality of ‘Sanuki Gold’ kiwifruit harvested at two different maturity stages were investigated. MA storage in both fruit harvested early at 136 days after pollination (DAP) or late at 154 DAP delayed flesh softening, increase in soluble solid concentrations (SSC), decrease in titratable acids (TA), and reduction in fruit flesh color index compared to air stored fruit, suggesting that MA storage is effective in prolonging ‘Sanuki Gold’ kiwifruit storage life. Further, MA stored fruit did not attain full ripening flesh firmness and SSC thresholds even after 4 months of storage under MA conditions, suggesting that early harvested ‘Sanuki Gold’ kiwifruit can be stored for 4 months in MA. Fruit from both harvesting maturity stages stored under air conditions achieved maximum SSC (18%) values during storage, suggesting that two weeks early harvesting did not compromise edible quality characteristics. Only late harvested fruit treated with 1-MCP and stored in MA showed slight inhibitory effect specific to fruit softening during the first and second month of storage, suggesting that 1-MCP may have some limited ripening inhibitory effect during storage of ‘Sanuki Gold’ kiwifruit.
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