Gametophytic self-incompatibility (SI), an important character for breeding seedless cultivars of
Citrus, is known in pummelo, mandarin, and many hybrid cultivars with pummelo in their pedigrees. Only a little is known about the allelic variation in the self-incompatibility gene (
S),
S allele frequencies, and the genotypes of self-incompatible and semi-self-compatible cultivars. In this study, pollination of ‘Banpeiyu’ S
1 seedlings with ‘Banpeiyu’ and pollination between the S
1 seedlings were performed to determine homozygous S
1 seedlings for the
S gene. Seventy-eight
Citrus accessions, including 55 pummelo accessions, were pollinated with each of two homozygous S
1 seedlings (
S1S1 and
S2S2). Pollen tube arrest in the style base of their pollinated pistils indicated that 23 accessions, including ‘Banpeiyu’, have an
S1 allele each and 16 accessions, including ‘Banpeiyu’, have an
S2 allele each. Frequency of accessions with
S1 allele was 29.9% (23 of 77 accessions examined) and
S1 allele frequency was 16.4% (23 of 140 alleles excluding
Sf allele). Frequency of accessions with
S2 allele was 21.3% (16 of 75 accessions examined) and
S2 allele frequency was 11.6% (16 of 138 alleles excluding
Sf allele). Pummelo accessions collected from Kagoshima Prefecture had
S1 alleles with two and half times higher frequency (56.3%) than that in all accessions examined. Of the 79 accessions, six accessions (‘Banpeiyu’, ‘Iriki Buntan’, ‘Kaopang’, Nagashima Buntan No. 6, Nagashima Buntan No. 7, and ‘Soyu’) were
S1S2 genotypes. The
Citrus cultivars, whose
S genotypes have been fully determined in this study, were ‘Banpeiyu’ (
S1S2), ‘Iriki Buntan’ (
S1S2), ‘Kaopang’ (
S1S2), ‘Soyu’ (
S1S2), ‘Kinukawa’ (
SfS2), and ‘Kawano Natsudaidai’ (
SfS2).
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