In this study, we cloned and sequenced PCR products from
S-RNase alleles of 12 cultivars to determine their
S-RNase genotypes or
S haplotypes. We identified a novel
S11-RNase, which was previously misidentified as
S6-RNase. In addition, the first and second intron sizes of
S1- to
S11-, and
Sf-RNases were presented. Since differences among the second intron sizes of
S3-,
S9-, and
S10-RNases, as well as those among
S5-,
S6-, and
S11-RNases, appeared to be very small, we developed primer sets for allele-specific PCR amplification to distinguish these
S-RNase alleles. Consequently, 12
S-RNase alleles could be distinguished by PCR amplification using consensus primers for the second intron and/or the allele-specific primer sets. Using these PCR amplification, we determined
S-RNase genotypes of 14 cultivars, including ‘Kagajizo’ (
S6S10), ‘Baigo’ (
S6S10), and ‘Orihime’ (
S11Sf), whose
S-RNase genotypes have been updated. The DNA sequence information for
S-RNases and the allele-specific primer sets developed in this study would be useful for future
S-RNase genotyping and thus
S haplotyping in Japanese apricot.
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