Aim: We investigated atherosclerotic effects in young healthy subjects according to SNPs in paraoxonase 1 (PON1) and adiponectin (ADPN).
Materials and Methods: A total of 217 young heal thy Japanese subjects aged between 18 and 31 years were enrolled in this study. The genotypes of PON1, Q192R (rs662) and L55M (rs854560)
were elucidated using the PCRRFLP method, and those of ADPN, +276G/T (rs1501299) and +45T/G (rs2241766) using an automated DNA sequence analysis. To investigate the relationship between genetic polymorphisms and phenotypes, a univariate analysis of the dominant/recessive model and allele frequencies was performed. Furthermore, a multivariate analysis of all gene polymorphisms, age, sex, body mass index, and blood biochemistry data was conducted.
Results: The 4 genotypes were as follows, 11.5% QQ, 43.3% QR, and 45.2% RR in PON1Q192R, 89.1% LL, 10.4% LM, and 0.5% MM in PON1 L55M, 47.0% GG, 43.3% GT, and 9.7% TT in ADPN +276G/T, 51.2% TT, 39.6% TG, and 9.2% GG in
ADPN+45T/G. The univariate analysis showed that ADPN+45T/G was associated with intimamedia thickness (IMT). The risk allele G showed a significantly increased IMT in both the dominant and recessive model (P=0.047, P=0.035). In the multi variate analysis, ADPN+45T/G was identified as an independent factor associated with IMT (P=0.003). In the multivariate analysis of ADPN levels, 2 SNPs (PON1Q192R and ADPN+276G/T) were identified as independent factors(P=0.039, P=0.020).
Conclusions: The present study suggests the small effects of genetic polymorphisms even in young healthy individuals.
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