In this study, we analyzed the displacement field for the bottom of porcine aortic endothelial cells on a silicon substrate using sliced images of cells obtained by confocal laser scanning microscopy (CLSM). We measured the coordinates of the feature points on CLSM images of the endothelial cell bottom before and after stretching of the silicon substrate and calculated the relative displacement of the feature points. Then, assuming uniform deformation, we calculated the principal stretches and the rotation angle. The proposed microscale analysis of the displacement field directly showed that the cell bottom was almost uniformly deformed similar to the substrate.

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