The present study involved experiments on mice with the objective of elucidating histological changes after extravasation of irritant agents, as well as the effects of applying a poultice on these changes. Macroscopically, no marked lesions such as redness or ulceration were observed after extravasation of an anticancer agent(cyclophosphamide)classified as an irritant. Histologically, findings such as degeneration of panniculus carnosus muscle and accumulation of inflammatory cells were observed in the subcutaneous layer on days 1 and 3 in the group in which no treatment was given after extravasation. On days 5 and 7, regeneration of panniculus carnosus muscle was observed. While similar changes were seen on days 1 and 3 in the group in which a cold poultice was applied after extravasation, inflammatory cells were also seen on day 5 in this group, and no regeneration of panniculus carnosus muscle was observed. In the group in which a hot poultice was applied, while degeneration of panniculus carnosus muscle was seen on days 1, 3, and 5, no accumulation of inflammatory cells was observed. Moreover, degeneration of panniculus carnosus muscle had disappeared by day 7. These findings indicate that, in cases of extravasation of irritant agents, tissue injuries occur in layers below the subcutaneous tissue, even if no macroscopic changes are seen. These findings also suggest that, as opposed to a cold poultice, a hot poultice may be a more effective method of care for extravasation under these experimental conditions.
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