Animal Eye Research
Online ISSN : 2185-8446
Print ISSN : 0286-7486
ISSN-L : 0286-7486
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Volume 24, Issue 3-4
Displaying 1-3 of 3 articles from this issue
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Review
  • Animal Models for Human Cataract with Special Emphasis on N-Methyl-N-nitrosourea-induced Rat Cataractogenesis
    Airo TSUBURA, Katsuhiko YOSHIZAWA, Katsuaki MIKI, Yuji OISHI, Katsuji ...
    Article type: Review
    2005 Volume 24 Issue 3-4 Pages 3-4_1-3-4_8
    Published: February 14, 2006
    Released on J-STAGE: September 12, 2013
    DOIhttps://doi.org/10.11254/jscvo.24.3-4_1
    JOURNAL FREE ACCESS
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    Cataracts, defined as opacification of the lens, are multifactorial in origin. Epidemiological studies have identified increasing age, female gender, exposure to non-ionizing or ionizing radiation, metabolic disorders (such as diabetes mellitus), drugs (such as corticosteroids and anticancer agents) as risk factors. Cataracts have been reproduced in animal experiments that have confirmed the association of these factors as cataractogenic and identified the mechanisms of action. Among the animal models of human cataract disease, the administration of N-methyl-N-nitrosourea (MNU), an alkylating agent, to rats damages the DNA of lens epithelial cells, leading to apoptosis and ultimately lens opacity. The sensitivity of lens epithelial cells to MNU is inversely related to the age of the animals. When 70 mg/kg MNU was administered intraperitoneally to 15-day-old rats, in 4 weeks time, nuclear as well as cortical cataracts developed; no mortality and no gender differences were seen. Since the MNU-induced 15-day-old rat model develops cataract rapidly and consistently, it may be useful in screening anticataractogenic agents.
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Original Report
  • Auto-immunogenicity of Rabbit Water-soluble Lens Protein and its Variants of Other Specieses
    Nobuyuki KANEMAKI, Kazutaka KANAI, Ken MIURA, Kei ONO, Hisataka OKUJIM ...
    Article type: Original Report
    2005 Volume 24 Issue 3-4 Pages 3-4_9-3-4_13
    Published: February 14, 2006
    Released on J-STAGE: September 12, 2013
    DOIhttps://doi.org/10.11254/jscvo.24.3-4_9
    JOURNAL FREE ACCESS
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    Auto-immunogenicity of water-soluble lens protein was investigated in Japanese white rabbits (Jla: JW). The protein was purified from 10-week-old female Jla:JW rabbits using Sepahrose CL-6B liquid chromatography. Another female Jla:JW rabbit of the same age was injected once in the interdigital subcutis with a mixture of water-soluble lens protein and Freund's complete adjuvant, and blood samples were collected at 2 weeks post-injection. Fractionation of lens protein and the presence of an antibody were determined using SDS-polyacrylamide gel electrophoresis and Western immunoblot analysis. Antibodies against αA-, βH- and γ- crystallin were detected by homozoic immunization with the water-soluble lens protein in rabbits. The rabbit anti-lens protein antibody was cross-reactive to the water-soluble rat and canine lens protein, specifi cally for αA- crystallin.
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Original Report
  • Electroretinograms in the Uveitis Model Induced by Autologous Water-soluble Lens Protein of Rabbits
    Nobuyuki KANEMAKI, Kazutaka KANAI, Rie EGAMI, Hisataka OKUJIMA, Shiro ...
    Article type: Original Report
    2005 Volume 24 Issue 3-4 Pages 3-4_15-3-4_21
    Published: February 14, 2006
    Released on J-STAGE: September 12, 2013
    DOIhttps://doi.org/10.11254/jscvo.24.3-4_15
    JOURNAL FREE ACCESS
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    To investigate the influence of the retinal function in lens-induced uveitis (LIU), we examined the electroretinograms (ERG) in the uveitis model of Japanese white rabbits (Jla:JW) induced by autologous water-soluble lens protein. The experimental LIU model was made as follows. Water-soluble lens protein was prepared from the lens of the right eye, which was removed by the extracapsular extraction method, and 10 days later a mixture of the prepared lens protein (10mg) and Freund's complete adjuvant was injected subcutaneously into the fingers. Then, 2 weeks late, the lens protein (800μg) sterilized by filtering was injected into the anterior chamber of the left eye (LIU group). Sterile physiological saline was injected into the anterior chamber as the control. The congested hypertrophy of the iris in LIU group was shown clearly for 7 days after the injection of the anterior chamber as compared with the control (P<0.05 or P<0.01). There was no difference between the LIU group and the control in the protein concentrations of the anterior chamber. The antibodies produced by all animals were α-crystallin antibodies, and their serum antibody titers did not differ in either group. There was no difference between the LIU group and the control comparing with each component of ERG (peak latency of a- and b-waves, amplitude of a- and b-waves).
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