The developmental competence in vitro of immature oocytes from mouse early preantral follicles in a simplified culture was investigated. The follicles ware obtained from 4-10 weeks and 14 days old mice, respectively. On day 12 of culture, most of cultured follicles ware survived (100% vs 97%) in this culture system. The granulosa cell outgrowth was observed in all of surviving follicles. The oocytes grown in vitro ware leaded to GVBD (84% vs 96%), and 44% of them reached Metaphase II, respectively. This results show that this culture system is valid in not only preantaral follicles from prepuberal mouse but also that of postpuberal periods.

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SDT雌ラットの糖尿病病態における性ホルモンの影響を検討するために、SDT雌ラットにおいて卵巣摘出および卵巣摘出後の雌性ホルモン投与を行い、体重および血糖値測定ならびに膵島病変観察を行った!その結果、卵巣摘出(OVX)群の体重は偽手術対照(sham operation)群および卵巣摘出後にestradiol benzoateを投与した群(EB投与群)に比べて有意な増加を示し、EB投与群の体重はsham operation群およびOVX群に比べて有意に低値を示した,OVX群の血糖値はEB投与群に比べて有意に高値を示し、EB投与群の血糖値はsham operation群およびOVX群に比べて有意に低値を示したが、各群の平均血糖値はほぼ正常範囲内であった。膵臓の病理組織学的観察では、sham operation群において膵島内および膵島周囲に炎症性細胞の浸潤、線維芽細胞の増生およびヘモジデリンの沈着が認められ、OVX群においてこれらの変化が増悪化した。一方、EB群においては膵島の構造は正常と差を認めなかった。以上の様に、エストロジェンはSDT雌ラットにおける糖尿病態の進行に対して予防的に作用している可能性が示唆された。

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The advantages of blastocyst transfer in mid-good responders include a reduction in multiple pregnancies due to single good-quality blastocyst transfer and an elevation of implantation and ongoing pregnancy rates due to establishment of the vitrification method. However, evidence is lacking as to whether blastocyst culture is effective in poor responders. In fact, few centers have adopted blastocyst transfer for all patients. Blastocyst development rate is around 50-60%. Several kinds of commercial sequential media are currently available and almost all Assisted Reproductive Technology (ART) units use these for treatment of patients. However, the period in which these media can be used is limited to 26-70 days according to the instructions provided by manufacturers. The half-life of pyruvate and antibiotics is supposedly short, and vitamins and hormones are unstable. Ideally, centers would make and use their own media within 72 h. In the near future, optimal ovarian stimulation, culture conditions, embryo transfer and luteal support should enable the possibility of providing healthy pregnancy following single blastocyst transfer in almost all patients.

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