Propulsive activity in segments of isolated distal colon of guinea-pig was investigated. Propulsive activity, as indicated by movements of the bolus (5×10 mm diameter) made of plastic with a steel wire, was recorded isometrically using a force-displacement transducer. Muscle tension of longitudinal axis was also recorded isometrically in the same preparation.
In all preparations, spontaneous propulsive activity, which was due to combination of ascending excitation and descending inhibition, occured periodically.
The frequency of propulsive activity was 4.0±0.9/10 min (M±SE, n=8) and the duration was 64.0±9.0 sec (M±SE, n=9). The propulsive force was 16.4±3.4 g (M±SE, n=10) at peak.
The spontaneous propulsive activities were blocked by atropine (10^-5 g/ml). These activities were gradually decreased in frequency and in propulsive force within 2-3 hours.
When spontaneous propulsive activities were negligible, carbachol (10^-9-10^-7 g/ml) added to the bathing medium stimulated propulsive activity in a dose dependent manner.

抄録全体を表示

麻酔犬の消化管運動に対する作用を検討した結果, trimebutine maleate (TM-906) は静脈内投与および十二指腸内投与のいずれにおいても, 胃に対しては抑制ならびに亢進の両作用を示し, 小腸, 結腸に対しては亢進作用のみを示した.迷走神経切断後においてもTM-906は迷走神経切断前と同様の作用態度を示した.したがって麻酔犬の消化管運動にたいするTM-906の作用は主として末梢性の作用に基づくものと推測される.

抄録全体を表示

The Role of the striated urethral sphincter on the dynamics of micturition was studied using 4 decerebrated dogs. The voiding cycles were analyzed by pressure flow EMG and pressure flow plot studies before and after the administration of suxamethonium. In the control condition, highly reproducible reflex micturition with bladder contraction and spasmodic rhythmic sphincter contractions was demonstrated. After the administration of suxamethonium reflex micturition occurred, but there was decreased bladder emptying as well as absence of spasmodic rhythmic sphincter contractions. The striated urethral sphincter would seem to play an important role in bladder emptying of decerebrated dogs.

抄録全体を表示

The effects of trimebutine (2-dimethylamino-2-phenylbutyl-3, 4, 5-trimethoxy benzoate hydrogen maleate) and metoclopramide (N-diethylaminoethyl-2-methoxy-4-amino-5-chlorobenzamide) on propulsive activity of the isolated segmental colon, and on longitudinal and circular muscle layers of colon in guinea-pig were investigated.
Trimebutine in doses up to 10^-7g/ml slightly stimulated propulsive activity, but in doses as high as 10^-7g/ml inhibited it. However, metoclopramide (10^-7-10^-5g/ml) stimulated propulsive activity in a dose dependent manner. Neostigmine-stimulated propulsive activity was inhibited by trimebutine (10-6g/m/) but potentiated by metoclopramide (10^-5g/ml).
Trimebutine (10^-8-10^-5g/ml) contracted circular muscle layer in a dose depen-dent manner, and contracted longitudinal muscle layre in doses up to 10^-7g/ml, but in doses as high as 10^-7g/ml relaxed it. While, metoclopramide (10^-8-10^-5g/ml) contracted both muscle layers.
These results indicate that, at high doses, trimebutine-induced inhibition of propulsive activity may depend on a relaxation of longitudinal muscle, and at low doses, trimebutine-induced stimulation of propulsive activity may depend on a contraction of longitudinal muscle which was partly inhibited by atropine (10^-6g/ml), though metoclopramide at low doses had little effect on propulsive activity.

抄録全体を表示