Nutritional environment for mycelial growth in Hypsizygus marmoreus was examined. Optimum temperature and initial pH of medium for the vegetative growth were 25℃ and 7.0, respectively. Glucose was especially good carbon source for mycelial growth. Sucrose, trehalose, starch, xylose and maltose were also acceptablecarbon sources. Potato extract (200 g potato/L) and dried bear yeast were the most effective nitrogen source for the mycelial growth. However, casamino acids, malt extract and glutamic acid were poor as nitrogen for this mushroom. Intra- and extra-cellular hydrolytic enzyme activities during vegetative growth of this mushroom were examined on potato glucose liquid medium. The activity of β-1, 3-glucanase in culture filtrate greatly increased by prolonging the incubation time. CM-Cellulase and avicelase activities gradually increased with the vegetative growth. In contrast, CM-cellulase and avicelase extracted from mycelia showed higher activity than those in the culture filtrate. The activities of neutral and acid proteinases, chitinase, N-acetyl-β-D-glucosaminidase also were detected in the mycelia, and only chitinase activity greatly increased from 25 to 30 days after inoculation. But, the productivity of β-1, 3-glucanase in the mycelia showed considerably lower level. Proteinases and neutral proteinase showed the higher activity levels than acid proteinase both in the mycelia and the culture filtrate. From these results, some possible roles of these enzymes are discussed.

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