The purpose of this investigation is to make clear the relation between resistance level to Phenkapton and character of E
1 esterase detected by thin layer electrophoresis.
Activity of E
1 esterase was calculated as follows. The size and colour density of each band, which was thought to signify the activity of each separated esterase, were transformed to the area enclosed with density curve by densitometer, and then the rate of the area of E
1 esterase band to the whole was calculated.
Resistance level to Phenkapton was estimated from the corrected mortality (Abbott) of eggs. By using β-Naphthyl acetate as a substrate, the esterase was separated into four bands, which were designated as E
1, E
3, E
4 and E
5 band. The colour density and area of E
1 band as the activity of E
1 esterase, showed individual difference. All the individuals of following four hybrid progenies showed presence of E
1 band; RR×R, F
1 (RR×S)×R, RR×S and SS×R. In F
1(SS×R)×S progeny about 65% of individuals showed presence of E
1 and the susceptible strain SS×S showed no E
1. Therefore, it may be said that the mite having the resistant factor even as a heterozygote, showed E
1 band, and the individuals without resistant factor did not show. Difference of the activity of E
1 esterase was observed between male and female of resistant strain. The male showed higher activity than the female. The activity of E
1 esterase of the hybrid progeny of resistant and susceptible strains was lower than pure line of resistant strain.
The activity of E
1 esterase correlated closely with resistance level to Phenkapton. A correlation coefficient of r=-0.854 was obtained.
In conclusion, the E
1 esterase is responsible for genetic factor of Phenkapton resistance, and the resistance level depended on the rate of E
1 esterase activity.
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