Kyushu Plant Protection Research

In vitro screening assay of resistance to bacterial wilt caused by Ralstonia solanacearum in potato with sterile cultured plants

[in Japanese], [in Japanese]

2014 Volume 60 Pages 1-8
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.1

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In order to establish an assay for bacterial wilt resistance in vitro for potato plants, we examined the assay conditions of inoculation with Ralstonia solanacearum. The suitable plant stage and bacteria concentration as inoculum were the 4-5 leaf stage and 10² CFU/ml in this assay. The suitable incubation temperature was also 28℃ after inoculation. The validity of this assay was assessed using six cultivars and two breeding lines of potato plants with known resistance to R.solanacearum in field screening. Cultivars（or breeding lines） with fewer dead plants in the assay（lower dead plant rates）had stronger resistance in the field test. In addition, this assay was able to distinguish statistical differences between the resistant cultivars（or breeding lines）and the susceptible cultivars in the field screening. These results suggest that this assay system is effective for evaluating bacterial wilt resistance in potato plants.

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The disease suppressive effect of chitosan oligosaccharide against tomato late blight

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

2014 Volume 60 Pages 9-19
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.9

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A bioassay to test the sensitivity to several fungicides for the causal agent of strawberry anthracnose by using detached leaves

[in Japanese], [in Japanese]

2014 Volume 60 Pages 20-23
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.20

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Acibenzolar-S-methyl （ASM） drenching treatment of strawberry mother plants and nursery plants have a potentiality to control strawberry anthracnose

[in Japanese]

2014 Volume 60 Pages 24-29
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.24

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Survival of powdery mildew in strawberry nursery in summer

[in Japanese]

2014 Volume 60 Pages 30-36
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.30

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Survey of Citrus Huanglongbing （greening disease） on the Amami Islands. 3. Transition of diseased tree distribution after the second survey

[in Japanese], [in Japanese]

2014 Volume 60 Pages 37-42
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.37

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Citrus greening disease （CG） caused by 'Candidatus Liberibacter asiaticus' （Las） was investigated on Amamioshima, Tokunoshima, Okinoerabu and Yoron islands of Kagoshima in Southern Japan. Based on the distribution of infected trees in the second investigation from April 2006 to March 2009, citrus trees on four islands were categorized. A total of 143,782 citrus trees were investigated at 22,866 areas on four islands during the third investigation from April 2009 to March 2012. A total of 8,694 symptomatic trees at 6,569 areas were examined for Las infections by polymerase chain reaction （PCR）. As a result, 258 trees at 120 areas were confirmed to be infected. Infected trees and newly confirmed areas with infected trees in this survey were fewer than those in the second one on Tokunoshima and Yoron islands. The frequencies of uninfected/infected trees during this survey were calculated and compared with those during the second one. Odds ratios indicate that infected trees and newly confirmed areas with infected trees in this survey were fewer than those in the second one on Tokunoshima and Yoron islands. In order to detect latent infected trees, monitoring of CG is discussed.

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Occurrence of QoI-resistant Plasmopara viticola, the casual agent of grapevine downy mildew, in Fukuoka Prefecture and control with fungicides

[in Japanese], [in Japanese], [in Japanese]

2014 Volume 60 Pages 43-47
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.43

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Control of stem-end rot of mango by heat treatment of the peduncle

[in Japanese], [in Japanese]

2014 Volume 60 Pages 48-52
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.48

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To develop an effective method for controlling post-harvest stem-end rot of mango, we investigated the efficacy of heat treatment of mango peduncle using a soldering iron. Examination of pathogen progression from the peduncle to fruit interior in fruits of varying degrees of maturity revealed that the pathogen developed more quickly in mature fruit than in immature fruit, with the pathogen in the former advancing to a depth of 15 mm from the peduncle 12 h after inoculation with the pathogen. Heat treatment of the peduncle was accomplished by cutting the tip of the soldering iron flat and pressing the heated tip against the peduncle for 10 s. Following heat treatment of peduncles 3, 6, 12, or 24 h after inoculation with the pathogen, disease was significantly inhibited only in fruits that were heat treated 3, 6 or 12 h after inoculation, but not in those that were heat treated 24 h after inoculation. These results demonstrated that it is necessary to perform heat treatment of peduncles within 6 h of harvest in order for the treatment to be effective. In addition, in field experiments in which peduncles were heat treated 4 h after harvest, we observed marked inhibition of disease in heat-treated fruit compared to untreated fruit, with no deterioration in quality of heat-treated fruit.

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Occurrence of southern blight disease on Cleyera japonica by Sclerotium rolfsii Saccardo

[in Japanese]

2014 Volume 60 Pages 53-56
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.53

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Blight-like symptoms on Cleyera japonica Thunb. were first found on plants growing on the shrine in Omura, Nagasaki, Japan on June 19, 2009. White mycelia and brown, spherical, rapeseed-sized sclerotia were observed on the diseased plants. A fungus similar to Sclerotium rolfsii Saccardo was isolated from infected plants. The optimum growth temperature of these isolates, on potato dextrose agar, was 25℃for mycelial growth and 30℃for sclerotium formation. The mycelial tufts were white to grayish brown, and the main hyphal width ranged from 4.5-7.8μm（avg. = 6.6μm）with clamp connections. These mycological characters are identical to those of S. rolfsii reported in other plants. The isolates were found to be pathogenic to C. japonica in artificial inoculation test, and could be re-isolated from infected tissue. It is proposed that the disease should be referred to as southern blight of C. japonica caused by S. rolfsii.

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Occurrence of Southern rice black-streaked dwarf （SRBSDV） in paddy fields transplanted at different times

[in Japanese], [in Japanese]

2014 Volume 60 Pages 57-63
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.57

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Biology and management of the sweetpotato flea beetle(Chaetocnema confinis Crotch). 2. Seasonal timing of C. corfines larvae make damage on tuberous root of field sweetpotato in Tanegashima Island, Kagoshima.

[in Japanese], [in Japanese], [in Japanese]

2014 Volume 60 Pages 64-67
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.64

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Geographic distribution of the sweetpotato weevil, Cylas formicarius （Coleoptera: Brentidae） and the West Indian sweetpotato weevil, Euscepes postfasciatus （Coleoptera: Curculionidae） on the Amami Islands （2010-2011)

[in Japanese]

2014 Volume 60 Pages 68-74
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.68

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The geographical distribution of the sweetpotato weevil, Cylas formicarius and the West Indian sweetpotato weevil, Euscepes postfasciatus were investigated on sweetpotato, Ipomoea batatas and wild host plants, I. india and I. pes-caprae on the Amami Islands except Kikai Island during 2010 to 2011. The number of C. formicarius male adults was determined using simple sticky traps with synthetic sex pheromone at 235 sites during November 2010 to February 2011. The occurrence of the two weevils were investigated at 109 sweetpotato fields and 97 creeping colonies of wild host plants, I. india and I. pes-caprae, sites during November 2010 to February 2011. Both weevil species were found in all islands of the Amami Islands. This was consistent with the result of 1997-1998, and the distribution of both weevils species have not changed on the Amami Islands. The occurrence of E. postfasciatus was on the increase from 1997. On Okinoerabu Island, the number of male adults of C. formicarius captured using pheromone traps were fewer than the other islands.

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Decline in susceptibility to diamide insecticides of the diamondback moth , Plutella xylostella L.(Lepidoptera), collected in Kagoshima Prefecture

[in Japanese], [in Japanese]

2014 Volume 60 Pages 75-78
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.75

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Effect of some insecticides on Frankliniella occidentalis collected in Oita Prefecture and the first confirmation of a decline in susceptibility to Spinosad

[in Japanese], [in Japanese], [in Japanese]

2014 Volume 60 Pages 79-83
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.79

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Effects of field application of several insecticides and dose of spray solution deposition on abaxial surfaces of chrysanthemum leaves on efficiency of Thrips nigropilosus (Thysanoptera: Thripidae) control

[in Japanese], [in Japanese], [in Japanese]

2014 Volume 60 Pages 84-89
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.84

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[title in Japanese]

2014 Volume 60 Pages 90-91
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.90

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[title in Japanese]

2014 Volume 60 Pages 92-98
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.92

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[title in Japanese]

2014 Volume 60 Pages 99-105
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.99

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[title in Japanese]

2014 Volume 60 Pages 106-124
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.106

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[title in Japanese]

2014 Volume 60 Pages 125
Published: November 28, 2014
Released on J-STAGE: April 22, 2016

DOIhttps://doi.org/10.4241/kyubyochu.60.125

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