The transmittance of protein solutions contained in a number of test tubes was estimated after they were arranged in a line and left for several hours in this state. It was found that the transmittance of the protein solution in each test tube altered during the time when the test tubes had been left in this way.
The pattern of the transmittance-difference along the line of the test tubes varied with the direction of the line.
Frequently, the transmittance increased or decreased either in a regular or in a zigzag manner along the direction of the line.
The pattern was repeatedly found to be pronounced in the direction extending north and south, and in most cases it varied with the day of the observation.
Identical phenomena were demonstrated with test tubes containing culture solution in which Proteus vulgaris had been inoculated. In this case, analogous patterns were observed in the difference of the growth rate of the bacteria. It was found that on a certain day when the pattern of the protein solution was very pronounced in the direction north and south, the pattern of the bacterial growth was likewise very striking in the same direction.
This phenomenon suggests that the X-agent has a tendency to progress along a certain direction which may change more or less from time to time.

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It has been observed that the presence of air greatly influences the assimilation of minerals, Mg⁺⁺, Ca⁺⁺ and PO₄″' Under anaerobic condition, mineral intake for the formation of one gram of yeast cells is high as compared to that under aerobic condition.
The yield of yeast is slight in culture media which were kept under anaerobic conditions. Maximum yield of yeast cells is observed at 53.0mgm of PO₄″' concentration per 200ml of the culture medium in both aerobic and anaerobic conditions.
Further, it is interesting to note that at the concentration of PO₄″' in the culture medium which forms maximum growth of yeast cells, the assimilation of Ca⁺⁺, Mg⁺⁺ and PO₄″' is least and also the consumption of sucrose and remains at a minimum. This has been found true for both aerobic and anaerobic conditions.

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Trichomycin exerted a considerable inhibitory effect upon the deamino-oxidation of such L-amino acids as L-alanine, L-proline, L-arginine, L-glutamic and L-aspartic acid by C. albicans in the concentrations below the minimal effective amount necessary in fungistasis. Furthermore, the oxidation of D-alanine and D-serine was completely or nearly completely inhibited by the addition of minute amounts of the drug. Also, the inhibiting action of the drug upon the metabolism of those L-and D-amino acids increased more and more with the lapse of time, and the inhibition rate of the drug constantly showed a high level rate.
Those respirometric data strongly suggest that trichomycin irreversibly inhibits, as an enzyme inhibitor, the deaminooxidation of amino acids by C. albicans, and the respiration inhibiting action of the drug is both more evident and more specific on the amino acid metabolism than that on the aerobic carbohydrate dissimilation. In regards to the mechanism by which the respiratory metabolism in the living cells of this organism is prevented by the addition of minute amounts of trichomycin, it is supposed that the drug may act on the flavin enzyme system which is directly connected with oxygen.

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The fixation of the cobalt ion to Escherichia coli was studied qualitatively as well as quantitatively. The radioactive cobalt ion adsorbed to the cells was greatly lost during the first washing, and to a lesser extent in the second and third washings; therefore, the adsorbed cobalt ion consists of two forms; namely, the slightly absorbed and the firmly fixed forms. The firmly fixed cobalt ion was measured by referring to the cultural conditions of cells, pH, and the concentration of the isotope and the cells.

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Antigenic relationships between human and bovine tubercle bacilli and atypical mycobacteria were investigated by means of the Ouchterlony method using a culture media of these organisms as antigen, and the following were obtained:
1. The homologous reaction between H37Rv antigen and its antiserum produced three or four separate bands of precipitation. However, the reaction between H37Rv antiserum and antigen of other human tuberculous strains developed only one band of precipitate which confluenced with the first band between H37Rv antiserum and homologous antigen. The above human tuberculous strains were isolated from sputum of tuberculous patients seven years ago.
2. The reaction between the antigen of bovine tuberculous strains and antiserum of H37Rv produced only a single band, and the precipitation between the antigen of atypical and H37Rv antiserum showed only one band.
3. No reactions were observed between antigen of fresh milk strains and antisera of human tuberculous strains, but this antigen reacted with the antisera of bovine tubercle bacilli.
4. Common antigen exists between human and bovine tubercle bacilli as well as atypical mycobacterial strains.
5. Antisera of fresh milk strains did not react with the antigens of human and bovine tuberculous strains as well as with the atypical strains (1-5).
6. Antibodies against some atypical mycobacterial strains were detected in the serum of one of five cases of tuberculous patients.
7. Evaporated Dharmendra antigen showed a precipitate band with antisera of the atypical strains (1-2), H37Rv and bovine strains.

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1) D-Glucuronic acid, D-glucuronolactone and ethyl D-glucuronate were decomposed by members of the colon-typhoid group of bacteria, Penicillium, Aspergillus and Streptomyces.
2) D-Glucuronamide could not be degraded by any organisms tested.
3) D-Glucuronic acid, D-glucuronolactone and ethyl D-glucuronate exhibited the growth-promoting effect on bacteria which could decompose them.
4) D-Glucuronamide also stimulated the growth of bacteria.
5) E. coli B-19 proved to decompose D-glucuronic acid, D-glucuronolactone and ethyl D-glucuronate adapitvely, but not n-glucuron amide.

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