As the result of our chemical studies on the specimens of Sphaerophorus collected in Japan, we could find chemical strains in S. fragilis, S. meiophorus and S. melanocarpus.
a) Among 20 specimens of S. fragilis, we found that 1 specimen contains sphaerophorin only, 18 specimens contain sphaerophorin and squamatic acid, and 1 specimen contains sphaerophorin and squamatic acid and one undetermined ingredient which is found in foreign specimens of S. fragilis, S. globosus and S. tener.
b) Among 34 specimens of S. meiophorus, 3 specimens contain sphaerophorin only, and 31 specimens contain sphaerophorin and squamatic acid.
c) Among 28 specimens of S. melanocarpus, 3 specimens contain sphaerophorin only and 25 specimens contain sphaerophorin, stictic acid and constictic acid.
d) Among 7 specimens of S. diplotypus and 16 specimens of S. fragilis collected in Japan, all specimens contain sphaerophorin, stictic acid and constictic acid, and no chemical strain was found.
e) Among 7 specimens of S. turfaceus all specimens contain sphaerophorin only and no chemical strain was found.
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