-
Article type: Cover
1985 Volume 31 Pages
Cover1-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
App1-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
App2-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Index
1985 Volume 31 Pages
Toc1-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Index
1985 Volume 31 Pages
Toc2-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
App3-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
App4-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
App5-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
1-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Teiji IIJIMA
Article type: Article
1985 Volume 31 Pages
2-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Hiroshi SOZU
Article type: Article
1985 Volume 31 Pages
3-4
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
A. P. MacKenzie
Article type: Article
1985 Volume 31 Pages
5-8
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
9-18
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Teruyuki FUJITA, Kisook KIM
Article type: Article
1985 Volume 31 Pages
19-23
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Osato MIYAWAKI, Toshimasa YANO
Article type: Article
1985 Volume 31 Pages
24-26
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Takeshi SAKANE, Ko IMAI, Isao BANNO
Article type: Article
1985 Volume 31 Pages
27-35
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Toshiki MORICHI, Tokuzo KITADA, Ichiro SUZUKI, Keiko S. Yoshida, Misao ...
Article type: Article
1985 Volume 31 Pages
36-39
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Kazuo KIMURA, Kaname SAIDA, Yoshihiro NAKAMURA
Article type: Article
1985 Volume 31 Pages
40-45
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
The viability of Xanthobacter autotrophicus strain N34 and its oxygen-resistant segregant, strain Y38, was investigated after freezing and freeze-drying. The maintenance of oxygen-resistance of strain Y38 was also estimated by the results of plate cultivation method and liquid cultivation method under high oxygen tension. The viability of both strains immediately after freeze-drying was about 50%, while that after freezing was over 95%. Moreover, the viability of freeze-dried cells of both strains after storage for 24 months decreased markedly, showing that X. autotrophicus is highly sensitive to freeze-drying as already reported. From the result of plate cultivation, oxygen-resistance of strain Y38 seemed to be completely maintained after freezing or freeze-drying. On the other hand, from the result of growth delay analysis in liquid cultivation, only 24% of cells maintained oxygen-resistance immediately after freeze-drying, while almost 100% was maintained after freezing. Moreover, after storage for 24 months oxygen-resistance of frozen or freeze-dried materials was markedly decreased. The difference between the results of two cultivations indicated that many nonlethally injured cells existed among apparent oxygen-resistant cells grown on the plate under high oxygen tension.
View full abstract
-
Kazuo KIMURA, Eiichi MIKAMI
Article type: Article
1985 Volume 31 Pages
46-52
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Yukio TSUCHIYA
Article type: Article
1985 Volume 31 Pages
53-62
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
Viability of conidia or sclerotia of various fungi subjected to L-drying and freeze-drying was tested after storage for 47 to 209 days at 5℃ and 47 to 203 days at 37℃, respectively. The viability of spores of obligate parasitic fungi subjected to programmed freezing and of mycelial disks of Mastigomicotina subjected to freezing at -80℃ in a freezer or in liquid nitrogen was tested immediately after treatment of after storage for 19 to 80 days. The conidia or sclerotia subjected to L-drying showed a high viability when L2 was used as suspending medium, although the conidia of Botrytis cinerea and Pyricularia oryzae showed a low viability after L-drying. The conidia and sclerotia subjected to freeze-drying showed a lower viability than after L-drying. The conidia of Pseudoperonospora cubensis subjected to programmed freezing showed a higher viability when 10% dimethyl sulfoxide (DMSO)was used as suspending medium. The conidia of Erysiphe pisi subjected to programmed freezing showed a very high viability when 5% DMSO+5% sucrose was used as suspending medium. The homogenized suspension of club root containing resting spores of Plasmodiophora brassicae subjected to freezing at -80℃ showed a high viability regardless of suspending media used. The mycelial disks of Phytophthora spp. subjected to freezing at -80℃ showed a high viability when 5% or 10% DMSO, 5% glycerol, 5% sucrose, or 5% DMSO+10% sucrose were used as suspending media, but the disks of P. capsici did not survive when subjected to the same tests. The viability of the mycelial disks of Pythium spp. fluctuated in the same tests. The mycelial disks of Phytophthora spp. and Pythium spp. lost their viability after being subjected to freezing in liquid nitrogen.
View full abstract
-
Teruyoshi HASHIBA, Yasuyuki TETSUKA, Takahito SUZUI
Article type: Article
1985 Volume 31 Pages
63-67
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
Protoplasts of Rhizoctonia solani isolated from mycelia were frozen either rapidly by direct immersion of the vials in liquid nitrogen or slowly followed by transfer into liquid nitrogen from a temperature of -60℃. The samples were thawed in water at 40℃. For successful recovery, protoplasts required slow freezing. 0.6 M sucrose in combination with 5% polyethylene glycol (PEG) or 5% ethyleneglycol (EG) were good cryoprotectants for protoplasts. Protoplasts treated with 5% dimethyl sulfoxide (DMSO) and 0.6M sugar (sucrose, mannose, lactose) allowed also 85 to 95% survival. This study demonstrates that with suitable protective solutions and proper handling the viability of a wide range of fungal protoplasts can be preserved in liquid nitrogen.
View full abstract
-
Article type: Appendix
1985 Volume 31 Pages
68-70
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Shigeo TANAKA
Article type: Article
1985 Volume 31 Pages
71-78
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
In the application of the extreme low temperature to medicine, essential of the cryotherapy/cryosurgery is a "High Energy Treatment", excellent in cost-benefit. Tissue temperature of -50℃ or tissue impedance of 500kΩ can definitely destroy the tumor. Cryosurgery provides dual prominent features: in situ destruction of the tumor which otherwise are difficult to treat, and the potential tumor-specific immunotherapy. Thus cryotherapy/cryosurgery should be incorporated into the "multi-disciplinary" regimen against cancer. Liquid nitrogen with a powerful cryogenic device must be used for the treatment of malignancies. And real-time monitoring during freezing will be a prerequisite for cryosurgery. Future problems include: clinical application of cryoimmunotherapy or cryoimmunointensification therapy, endoscopic cryosurgery as a closed surgery, and cryochemotherapy, a new mode of treatment.
View full abstract
-
Toshihiko MATSUBARA
Article type: Article
1985 Volume 31 Pages
79-81
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Soichi ARAI
Article type: Article
1985 Volume 31 Pages
82-84
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Toshio SAKUMA
Article type: Article
1985 Volume 31 Pages
85-87
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Sota HIRAGA
Article type: Article
1985 Volume 31 Pages
88-89
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Mitsuo TAKANO
Article type: Article
1985 Volume 31 Pages
90-96
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
Cells of Escherichia coli k-12 W3110 were freeze-dried with 5% Na-glutamate plus 1% sucrose, and sealed in ampoules in vacuo. The viability after accelerated storage test at a higher temperature was assayed by the growth delay analysis. Fitness of viability loss during the storage to three kinetic models were examined; (a)the monomolecular model or log(N/N_o)=-(k/2.303)t,(b)Tatematsu's model or log (N/N_o)=-A_t^<1/2>+C, and (C)Barbaree's model or log(N/N_o)=-A log t+C; where N or N_o is number of viable cells after or before storage; t is storage time; k, A and C are constants. Reported data using various organisms from other authors were also examined for the kinetic analysis. Fitness to the monomolecular model was similar to the other models. The fitness to every model was compared by the corelation coefficient. Decimal reduction time or D-value and the temperature dependence or Z-value were shown to be useful for estimation of stability during low temperature storage. Various microorganisms showed a similar Z-value of 16℃. Thus, a recommendable accelerated test was suggested.
View full abstract
-
Article type: Appendix
1985 Volume 31 Pages
97-98
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Bibliography
1985 Volume 31 Pages
99-144
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Index
1985 Volume 31 Pages
145-159
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Index
1985 Volume 31 Pages
160-167
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
168-169
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
170-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
171-172
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
173-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
174-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
189-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS
-
Article type: Appendix
1985 Volume 31 Pages
189-
Published: September 30, 1985
Released on J-STAGE: August 01, 2017
JOURNAL
FREE ACCESS