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Article type: Cover
1981 Volume 27 Pages
Cover1-
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Article type: Appendix
1981 Volume 27 Pages
App1-
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Article type: Index
1981 Volume 27 Pages
Toc1-
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[in Japanese]
Article type: Article
1981 Volume 27 Pages
1-3
Published: September 20, 1981
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Toshichika OHTOMO, Chizuko YOSHII, Kosaku YOSHIDA
Article type: Article
1981 Volume 27 Pages
4-8
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Shin-ichiro HAZE, Mitsuo TAKANO, Isao SHIBASAKI
Article type: Article
1981 Volume 27 Pages
9-11
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Tokio NEI, Rie OSHIRO
Article type: Article
1981 Volume 27 Pages
12-15
Published: September 20, 1981
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Takeshi SAKANE, Isao BANNO, Teiji IIJIMA
Article type: Article
1981 Volume 27 Pages
16-22
Published: September 20, 1981
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A study was conducted to make L-dried cultures escape from mutation during preservation. Since lesion of chromosomal DNA caused by preservation Eof L-dried cells is origin of mutation, if the cells are dried and preserved under such conditions so that DNA might be free from damage, mutation would not be induced in the dried cells. Such conditions of drying could also considerably prevent decrease of the survival of dried cells in recA^- strain which lack a system of post-replication repair of damaged DNA. Under this idea, a method was elaborated for selecting compounds which, when added to suspending medium, increase the survival value of L-dried cells of Escherichia coli B/r recA^- as an indicator, and 59 various compounds were screened. Adonitol, AICA, cysteine, dithioerythritol, semicarbazide, sodium thioglycollate and thiourea were found to be remarkably effective and adenosine, cystine, cytidine, erythritol, glutathione, thiomalic acid, thymidine and urea to have weakly protective effect. In dried cells of recA^+ strains, adonitol, cysteine and thiourea not only increased survival value but also prevented induction of mutation. Especially thiourea completely precluded mutation at a concentration of 30 mM.
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Naofumi HANAFUSA
Article type: Article
1981 Volume 27 Pages
23-26
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Fumiko NAKAZAWA
Article type: Article
1981 Volume 27 Pages
27-32
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Naofumi HANAFUSA
Article type: Article
1981 Volume 27 Pages
33-34
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Yasuyuki SAGARA, Akira HOSOKAWA
Article type: Article
1981 Volume 27 Pages
35-47
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Mitsutoshi HAMANO, Makoto OKAYASU, Hiroshi SUGIMOTO
Article type: Article
1981 Volume 27 Pages
48-53
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Kotaro HIEDA, Yoriaki MATSUDAIRA
Article type: Article
1981 Volume 27 Pages
54-58
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Sinji YUASA, Toshiyasu TSUKADA
Article type: Article
1981 Volume 27 Pages
59-65
Published: September 20, 1981
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Kiyoshi HAYAKAWA, Mitsuhiro SATO
Article type: Article
1981 Volume 27 Pages
66-72
Published: September 20, 1981
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The viability of Saccharomyces cerevisiae suspended in NaCl solution decreased markedly when the suspension was frozen to the temperature to form a eutectic mixture, whereas the suspension in CaCl_2 gave good survival of the cells. The difference between two groups of compounds, which involve NaCl and CaCl_2, respectively, on the freeze-thaw damage was studied. The viability of yeast cells decreased markedly when the formation of eutectic mixture was completed between about -10 and -40℃ in the solution of each compound. On the other hand, it hardly decreased when the formation of eutectic mixture was not completed in the range. These results suggest that compounds which had eutectic temperature in this range will give lower survival of yeast cells in the freezing process.
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Fumiyoshi FUKUMOTO, Hiroshi TOCHIHARA
Article type: Article
1981 Volume 27 Pages
73-78
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Yasuke TERASAKI
Article type: Article
1981 Volume 27 Pages
79-86
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Ryozaburo IRIE, Takashi OKAMOTO, Yasuhito FUJITA, Toshiki MORICHI
Article type: Article
1981 Volume 27 Pages
87-89
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Michiko KOBATAKE
Article type: Article
1981 Volume 27 Pages
90-99
Published: September 20, 1981
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Seventy nine yeast cultures of 53 different species belonging to 16 genera have been dried by a disc-in-bag method for preservating microbial cultures. Yeast cells were suspended in protective agent (serum broth), dropped on polyethylene foil, and dried in a laminar air flow bench for several hours. Dried discs were stored in a desiccator at room temperature for 51 to 56 months. After preservation, viable counts were carried out. With the exception of the death of only one strain, Torulopsis halonitratophila, all the other strains survived well. Of the 79 cultures tested 28 (35.4%) ranged from 10^7 to 10^8 viable cells per disc, and 49 (59.5%) ranged from 10^4 to 10^8 viable cells per disc. Morphological characteristics, fermentation reactions, assimilation of carbon compounds and nitrates, and growth temperatures of the 40 isolated cultures representing 11 genera after storage for 51 to 56 months, were compared with the data which was examined before drying. Cultures which were preserved have unalterably maintained the morphological and physiological characteristics they showed at the time of their original isolation.
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Daiji OKUNO, Yumiko KANAI
Article type: Article
1981 Volume 27 Pages
100-106
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Article type: Appendix
1981 Volume 27 Pages
107-110
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[in Japanese]
Article type: Article
1981 Volume 27 Pages
111-
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[in Japanese], [in Japanese]
Article type: Article
1981 Volume 27 Pages
111-112
Published: September 20, 1981
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[in Japanese]
Article type: Article
1981 Volume 27 Pages
112-
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[in Japanese]
Article type: Article
1981 Volume 27 Pages
112-113
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[in Japanese], [in Japanese]
Article type: Article
1981 Volume 27 Pages
113-
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1981 Volume 27 Pages
114-
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[in Japanese]
Article type: Article
1981 Volume 27 Pages
114-115
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[in Japanese]
Article type: Article
1981 Volume 27 Pages
115-
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[in Japanese]
Article type: Article
1981 Volume 27 Pages
115-116
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[in Japanese]
Article type: Article
1981 Volume 27 Pages
116-
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[in Japanese]
Article type: Article
1981 Volume 27 Pages
117-
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Article type: Bibliography
1981 Volume 27 Pages
118-139
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Article type: Appendix
1981 Volume 27 Pages
140-142
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Article type: Appendix
1981 Volume 27 Pages
143-145
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Article type: Appendix
1981 Volume 27 Pages
146-
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Article type: Appendix
1981 Volume 27 Pages
147-
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Article type: Appendix
1981 Volume 27 Pages
App2-
Published: September 20, 1981
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