Chemical sensors, such as biosensors, gas sensors, and odor sensors, respond specifically or selectively to chemical substances, outputting signals according to their amounts, and possessing diverse characteristics. For this reason, there is a strong demand for their use in various fields, including the environment, medicine, and food. Here, we first describe the basic structure of a typical chemical sensor, and then provide an overview of its response characteristics using Langmuir adsorption isotherms. Next, we present molecular sensing using transistors that utilize recognition reactions on functionalized graphene surfaces, developed by our research group.