We have isolated a virus strain from the brain of a patient who died of rabies in January 1948 following a very long incubation period. We are now continuing immunolog ical studies with this strain. In the course of experiments with mice aa curious variation phenomenon was noted, which seems to us to be a sufficient interest to merit description and discussion.

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The preceding paper (1) of this series includes reports on the biological properties of o-aminoph nol azo-tuberculin derivative prepared by coupling purified tuberculin (Maschmann) with diazotized o-aminophenol.
Due to the lamentable circumstances in Japan, which oppose a repetition of azo-tization experiments with purified tuberculin on a large scale, studies were being continued in our laboratory in an effort to find more simple method for obtaining o-aminophenol azo-tuberculin.
The present communication records the newly devised method for preparing highlyy active o-aminophenol azo-tuberculin derivative from “Sauton's synthetic-medium Old Tuberculin” itself.

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p-Aminosalicylic acid has recently been shown by Lehmann (1) to exert a strong inhibitory effect against tubercle bacilli (bovine type) in vitro and proposed as a possible chemotherapeutic agent against tuberculous infections.
Youmans (2) also reported that it is capable of inhibiting the growth of human tubercle bacilli. The p aminosalicylic acid effect appears to be specific for tubercle bacilli, according to Sievers (3) who studied several other species of Gram positive cocci and Gram negative organisms. Since the bacteriostatic effect of o-aminophenol appears also to be specifically directed against tubercle bacilli, as indicated by the findings of Okamoto and Matsuda (5), it would be of great interest to carry out comparative experiments with these two closely related compounds, p-aminosalicylic acid and o-aminophenol.
The present communication records the results of such experiment in brief.

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Reynolds, Schatz and Waksman (1) found an antibiotic substance, which was produced by several strains of S. griseus and adsorbed by activated carbon, and eluted. into neutral 90% alcohol, and they called this substance grisein. Thereafter, Garson and Waksman noticed, that there was two kinds of grisein, and the one was called grisein and the other grisein-like substance. Umezawa and his collaborators (2) recently isolated several strains of S. griseus which produced a grisein or grisein-like substance. Therefore, characters of these strains were studied in detail and described in this paper. All these strains were found to belong to S. griseus, however between the grisein-producing and the streptomycin-producing strains there was a noticeable difference during incubation in the synthetic solution containing various sugars. Streptomycin-producing strains did not grow in the synthetic solution containing glucose, but grisein-producing strains in general grow in it.

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Chloromycetin was first isolated by Smith, Gottlieb, Ehrlich, Bartz and others (1-3) and by Umezawa and others (4) separately. The chloromycetin-producing strains isolated by American authors were determined to be a new species as Streptomyces venezuelae (5) . Okami (6) of our laboratory designated our strain as Streptomyces pheochromogenus var. chloromyceticus. They seem to be identical each other. Later we isolated fourteen other chrolomycetin-producing strains which belong to the same species as the previous strains (7) . So the chloromycetinn production is one of the specific character of S. venezuelae or S. pheochromogenus var. chloromyceticus.
A strain which was presented in this paper was found to produce chloromycetin, although it is different from S. venezuelae or S. pheochromogenus var. chloromyceticus. The isolation and characters are described in this paper.

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