The existence of acid, neutral, and alkaline proteinases in fish muscle was examined. The species used were common mackerel, sardine, horse mackerel, white croaker, lizardfish, and deep-sea fish such as black halibut, soft eelpout, pectral rattail, and threadfin hakeling. Each proteinase activity was recognized in all fish ested. Specific activity of acid and neutral proteinases was large in the deep-sea fish, but tissue activity was small. The alkaline proteinase activity was recognized also in common mackerel and sardine, though the activity had not been detected previously in these fish. There was a fair difference among species in this specific activity; especially in sardines the activity was very large.
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