Chromosome number, amount of nuclear DNA, and nuclear volume were measured in 14 cases of the carcinoma of the cervix of the human uterus in which chromosome were clearly demonstrated out of 120 cases.
Following results were obtained on correlations in parameters measured and vaginal smear to the appearrance of chromosome demonstrated by Squash Method.
1) Chromosome number of the cancer cell of the uterine cervix exhibited a wide range of number variations from 35 to 136. The mode of chromosome number was observed in all cases. The highest peak of the frequency was noted at diploid range, particularly at hyperdiploid range. Another peak were noted at triploid and tetraploid ranges. It was decreased in sequence of triploid and tetraploid range, respectively.
Number of cases showing one mode was much higher than these having two modes.
2) While wide variation was noted in shape and size of chromosome of the cancer cell when compared to the normal in Karyotype analysis, no definit tendency was obtained in the cancer cell.
3) Amount of nuclear DNA in the cervical cancer cell was found to be higher than in normal cell and was distributed in wide range. Same tendencies were observed in frequency and type of mode of nuclear DNA as chromosome number. Amount of nuclear DNA was correlated to chromosome number.
4) Nuclear volume of the cervical cancer cell showed a wide variation, however same size as normal cell was also demonstrated. Nuclear volume was correlated to amount of nuclear DNA.
5) Chromosome number and nuclear DNA did not correlate to patient age, clinicalstage nor pathologic findings.
6) High apperance rate of chromosome by Spuash technique was obtained in the exopbytic type of cervical cancer in which appearance of malignant cell in vaginal smear was also high.
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