Dot-immunobinding assay (DIA) was useful for detecting Onion yellow dwarf potyvirus (OYDV) and Garlic latent carlavirus (GLV) from infected Welsh onions. The optimum concentrations of anti-OYDV and anti-GLV γ-globulins were 0.125 and 0.25μg/m
l, respectively. Under these conditions, the reaction end points of crude extracts of OYDV and GLV infected leaves were 1600-fold and 1280-fold, respectively, Viral antigen (VA) solutions were easily prepared from infected leaves as follows: The epidermis of infected leaves were peeled with razor blades. Two μ
l of buffer were put on the wound and mixed with the sap. The mixture was used as the VA solution. The VAs of OYDV and GLV, which were adhered to cellulose nitrate membrane, could be stored for 6 months in a desiccator and could be mailed without reduction of the color intensity of the DIA.
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