The purpose of this study was to develop the Negative Urgency Scale for Japanese adolescents, and to examine the influence of negative urgency and sensation seeking on adolescent desires for self-destructive behaviors (self-injury and risk taking behavior). Participants included 304 undergraduate and graduate students. The reliability of the scale was verified based on its internal consistency, while its concurrent validity was tested based on its correlation with physical and verbal aggression. In males, a hierarchical multiple regression analysis indicated that both negative urgency and sensation seeking were positively correlated with the desire to engage in both self-destructive behaviors. Furthermore, the score on the desire for self-injury was higher when the scores on negative urgency and sensation seeking were higher. In females, negative urgency was positively correlated with desire for self-injury, and sensation seeking was positively correlated with desire for engaging in risk taking behavior. However, the interaction between negative urgency and sensation seeking was not evident in females.