In January, 1953, there was an outbreak of swine influenza-like disease in Shizuoka Prefecture, and in July, 1953, another outbreak of the same disease in our institute.
In our laboratory, the virus was isolated from three materials of lungs with haemorrhagic lesions and consolidation by conducting intranasal inoculation into mice, and from another two materials by practicing amniotic method. As regards the elevation of antibody in a swine serum against the virus, the complement-fixation reaction and the haemagglutination-inhibition test were proved effective.
Our finding of the swine inoculated this virus is as follows: Observed were pathologically, oedem and haemorrhage of lung, follicle swelling of spleen, haemorrhage of urine bladder, swelling and congestion of each lymphnodes, and chinically, slight rise of temperature, (40-50.5°C), chilliness, decrease of appetite and coughing. Beside this, what were observed in circulating blood are such as the appearance of myelocyte and metamyelocyte, and the increase of rod nuclear cells in neutrophiles and of monocyte.
As a result of making tests in vitro and vivo on the newly isolated virus, we learned of its main properties as follows:
1. All the mice inoculated the virus through intranasal route, died after 4 to 8 days, presenting such a symptom as consolidation of lung, similar to that of influenza. Microscopically observed was catarrharic broach-pneumonia with severe infiltration of neutrophile leucocytes and necrosis of bronchial epithelcell. Virus multiplication was observed in spleen, lung and lymphnode.
2. As to the isolation of the virus from the swine, the amniotic method was successful, while the allantoic method was not. In case the virus was demonstrated by haemagglutination in the second or third serial amniotic passage, the cultivation of the virus in allantoic cavity and yolk sack of embryo could be made possible and the serial passage of the virus, made easy to develop through any route.
3. When the virus was inoculated into yolk sack of 6 to 8 day old embryo, the embryo, as a rule, was killed 48 hours after the inoculation; but it survived when the virus was inoculated on chorioallantoic membrane or in allantoic cavity.
Regardless of its route, the virus developed positively in 48 to 96 hours after the inoculation.
4. The virus titer in allantoic fluid usually indicated 10^-5 to 10^-7 and haemagglutination titer, 1: 1, 000-1:2, 000. And the haemagglutinative potency of the virus was demonstrated in the red cells of fowl, guinea-pig, sheep, goat, pig, mouse, pigeon, human (o type), rabbit and cattle, but it turned out negative in the red cell of horse.
5. The virus was completely adsorbed into fowl red cell at 4°C in 30 minutes and was eluted at 22°C in 10 hours.
6. The haemagglutinin was destroyed by heating at 50°C in 5 minutes, while the infectivity was entirely destroyed at 22°C in 30 days, and by heating at 50°C in 10 minutes.
7. In the process of isolation of the swine virus, the variation of phases “O” to “D”, which was described by Dr. Burnet et al. in the process of isolation of human influenza A virus, was observed. Outline of the variation of strain S was schematically shown in Chart No. 8. Also, in the case of isolation of strain 153, HME and 3471, the similar phenomenon was observed.
8. The filtrability of the virus, going through Seitz EK, Berkefeld w. and Chamberland L3, was proved.

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In the previous paper the authors reported on the isolation from the influenza-like disease of swine and the some properties of the virus. And, relationship between newly isolated virus and influenza virus of human type and NDV was reported. In this report, 17 young swines (weighing 8-10kg). 4 swines (weighing 18-22kg) and 3 swines (weighing 40-45kg) recieved by intraperitoneal, intravenous and intracerebral inoculation (as Table 1) 1ml of infected allantoic fluid (SEE strain was used), were observed as follows:
1. Clinically, some of swine developed a slight temperature, coughing, trembling, blear-eye, nasal discharge and diarrhoea, regardless of routes of inoculation and weighing. The many swines of weighing 8-10kg (i. n 6/8, i. v 3/3, i. p 2/3, i. c 3/3) were observed the stopping of growth and severe leaness. And the some of these swines (i. n 2/8, i. v 3/3, i. p 1/3, i. c 3/3) died. But, did not observed these symptoms in the all swines of above 18kg.
2. In haematological changes, the appearance of myelocyte, metamyelocyte and the slight increase of rod nuclearcells in neutrophiles were observed. Also, moderate increase of monocyte was observed. White blood corpuscles decreased to some extent in the some swine.
3. The virus was recovered at 7 and 17 days after inoculation from the lung and at 7, 17 and 52 days from the trachea by amniotic method.
4. Fig. 2 showed antibody response of young swine inoculated intranasally. H. I antibody achieved at 2 weeks after inoculation to maximum levels and lost already at 5 weeks. C. F. T antibody achieved at 4 weeks to maximum levels and lost at 6 weeks. Neutralizing antibody achieved at 2 weeks to maximum levels, but did not lose at 6 weeks.
5. Microscopically, 9 cases inoculated with this virus intranasally were investigated. In the lung of 8 case, the interalveolitis (which show the thickness of alveolar septum.), bronchiolitis and peribronchiolitis and in 1 died case and in the lung of all the cases inoculated intracerebrally, intraperitoneally and intravenously, congestion, haemorrhage and edema were observed. In other organ, the changes which based on circulatory disorder, atrophia of follicle (8/8) and haemorrhage of medullary cord of spleen (8/8), haemorrhage (7/8) and central necrobiosis of liver (3/8), haemorrhagic glomerulonephritis (4/8), interstitial nephritis (4/8), haemorrhage (6/8) and hyperplasia of reticulumcells of lymphnode, perivascular cuffing and hyperplasia of gliacells (1/9) were observed.

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