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Angiocardiographic Estimation of Left and Right Ventricular Volume Characteristics in Normal Infants and Children

TOMOHARU AKIBA, MASARU YOSHIKAWA, MASAHIKO KINODA, SHINSUKE OTAKI, KAZUMOTO ISHII, YOKIO KOBAYASHI, TETSUO SATO

The Tohoku Journal of Experimental Medicine
1985年 146 巻 3 号 313-320
発行日: 1985年
公開日: 2006/08/31

DOI https://doi.org/10.1620/tjem.146.313

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AKIBA, T., YOSHIKAWA, M., KINODA, M., OTAKI, S., ISHII, K., KOBAYASHI, Y. and SATO, T. Angiocardiographic Estimation of Left and Right Ventricular Volume Characteristics in Normal Infants and Children. Tohoku J. exp. Med., 1985, 146 (3), 313-320-Left (LV) and right ventricular (RV) volume data were obtained from biplane cineangiocardiograms in 31 patients with Kawasaki disease who showed normal coronary arteries in selective coronary arteriograms. LV and RV volumes were calculated by using Simpson's rule method. Both of end-diastolic (EDV) and stroke volume (SV) were excellently expressed as a function of body surface area (BSA) with exponential relationship: LVEDV=104.3 (BSA)^1.61
ml
(
r
=0.93, p<0.001), RVEDV=109.7 (BSA)^1.68
ml
(
r
=0.90, p< 0.001), LVSV=68.1 (BSA)^1.60
ml
(
r
=0.89, p<0.001) and RVSV=66.8 (BSA)^1.76
ml
(
r
=0.86, p<0.001). LV ejection fraction (LVEF) averaged 66±1% (mean± S.E.), and RVEF 58±1%. Both of them showed no significant correlations with BSA. The normal values of EDV and SV in infants and children can be predicted from their BSA by using the exponential equations demonstrated by the present study. Thus, these values in pediatric patients with heart diseases could be evaluated in terms of percentage of normal.
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高分解能型質量分析計を用いたエストロゲンの迅速一斉分析法の確立

*岡本誉士典, 森葉子, 青木明, 神野透人

日本毒性学会学術年会
2020年 47.1 巻 P-236
発行日: 2020年
公開日: 2020/09/09

DOI https://doi.org/10.14869/toxpt.47.1.0_P-236

会議録・要旨集フリー

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Purpose: Long-term exposure to estrogens increases the incidence of gynecologic cancers (breast, ovarian, and endometrial cancers) in humans. During the carcinogenic processes, estrogens acquire DNA-damaging activity via metabolic activation. Our group has studied the carcinogenic mechanisms of estrogens using an animal model for estrogen-induced breast cancer. To understand the precise mechanisms, it is quite essential to measure the metabolic profile of estrogens. In this study, we developed a rapid and simultaneous quantification method for three estrogens (estrone, E1; 17ß-estradiol, E2; 17α-estradiol, αE2) using a high-resolution mass spectrometer equipped with ultra-high-performance liquid chromatography (UHPLC-MS).
Methods: UHPLC-MS analysis of E1, E2, and αE2 was performed on a quadrupole time-of-flight MS (Triple TOF6600) and UHPLC (Nexera XR). Estrogens were quantified using a high-resolution multiple reaction monitoring as follows: E1 (m/z 269.1>145.069), E2/αE2 (m/z 271.1>145.069).
Results and discussion: Our method achieved a fast analysis (run time, 5 min), and good sensitivity and linearity as follows: E1 (1-1000 pg/
mL
,
r²
=0.9987), E2 (2-1000 pg/
mL
,
r²
=0.9991), αE2 (1-1000 pg/
mL
,
r²
=0.9989). Recovery test using a charcoal-stripped serum spiked 10-1000 pg/mL of estrogens showed a suitable recovery efficiency from 85.6% to 106.6%. Since the serum level of estrogen ranges from several to several hundred pg/mL, our method would be applicable for direct measurement of estrogen profiles without chemical derivatization.

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キャピラリー電気泳動法による胃腸薬中のロートエキスの迅速定量

小里一友, 三方比奈子, 鶴森敏樹

YAKUGAKU ZASSHI
1999年 119 巻 11 号 868-879
発行日: 1999/11/01
公開日: 2008/05/30

DOI https://doi.org/10.1248/yakushi1947.119.11_868

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A rapid and simple determination of tropane-alkaloids (hyoscyamine, scopolamine) in gastrointestinal drugs was investigated by capillary electrophoresis. Micellar electrokinetic capillary chromatography (MEKC) using a fused silica capillary (560 mm×0.075 mm i.d.) in 0.1 M SDS 20 mM borate buffer (pH 10)/acetonitrile (97 : 3) gave complete separation of the two alkaloids within 40 min. A sample solution was introduced by pressure method (50 mbar, 3.7 s), separation conditions applied voltage 15 kV and on-column detection was performed at 210 nm. Calibration curves for hyocyamine and scopolamine showed a good linearity in the range of 4-12μg/
ml
(
r
=0.9970) and 390-1150 ng/
ml
(
r
=0.9976). The present method is applicable to the simple and rapid determination of hyoscyamine and scopolamine in commercial gastrointestinal drugs.
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Comparison of Automatic Boundary Detection and Manual Tracing Technique in Echocardiographic Determination of Left Atrial Volume

Gui Can Zhang, Takanori Tsukada, Satoshi Nakatani, Masaaki Uematsu, Yoshio Yasumura, Norio Tanaka, Yoshikazu Masuda, Kunio Miyatake, Masakazu Yamagishi

JAPANESE CIRCULATION JOURNAL
1998年 62 巻 10 号 755-759
発行日: 1998年
公開日: 2001/09/25

DOI https://doi.org/10.1253/jcj.62.755

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Previous reports have indicated that echocardiography with automatic boundary detection (ABD) is useful for the noninvasive estimation of left ventricular volume. However, few data exist regarding the measurement of left atrial (LA) volume, which also provides pivotal information in the clinical setting. Therefore, the feasibility of LA volume measurement by ABD in comparison with the manual tracing using modified Simpson's method (SM) was evaluated. Fifty-nine patients with coronary artery disease with sinus rhythm were examined. Using ABD, a region of interest was set around the LA border and mitral annulus from an apical four-chamber view. The maximal and minimal LA volume (Vmax and Vmin) were measured from the volume waveform. Using the SM, the maximal and minimal LA volume were measured by the manual tracing on frozen frames at the apical four-chamber view. The ABD displayed a curve of LA volume change that consisted of passive emptying, diastasis, and active emptying phases during the left ventricular diastolic period. Under these conditions, the Vmax and Vmin were 43.7±11.2 ml and 21.1±7.6 ml, respectively, yielding the volume change of 22.6±6.0 ml. By the SM, Vmax and Vmin were 43.1±9.9
ml
{
r
=0.94, p<0.0001, y (ABD) = 0.91x (SM) + 3.6} and 22.0±9.0
ml
(
r
=0.91, p<0.0001, y = 0.94x + 0.7), respectively, and the volume change was 22.8±6.1
ml
(
r
=0.82, p<0.0001, y = 0.84x + 3.8). These results indicate that the ABD from the apical four-chamber approach could provide an accurate estimation of LA volume change, suggesting the potential value of this method in assessing LA function, although some technical difficulties need to be further overcome. (Jpn Circ J 1998; 62: 755 - 759)
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Oxidative Stress Correlates with Left Ventricular Volume after Acute Myocardial Infarction

Hiroyuki Fujii, Masami Shimizu, Hidekazu Ino, Masato Yamaguchi, Hidenobu Terai, Hiroshi Mabuchi, Ichiro Michishita, Akira Genda

Japanese Heart Journal
2002年 43 巻 3 号 203-209
発行日: 2002年
公開日: 2002/08/20

DOI https://doi.org/10.1536/jhj.43.203

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It has been suggested that oxidative stress may play a role in the pathophysiology of heart failure. However, little is known about the clinical relationship between oxidative stress and left ventricular dilatation after acute myocardial infarction (AMI). We prospectively studied 28 consecutive patients, successfully treated with primary coronary angioplasty, after their first AMI. To evaluate oxidative stress, plasma oxidized low-density LDL levels (U/mL) were measured serially 1 day, 7 days, 14 days, 30 days, and 90 days after the onset of AMI using a specific sandwich enzyme-linked immunosorbent assay. Left ventriculography and coronary angiography were obtained in all patients 3 months after the AMI and infarct-related arteries were all patent. Peak plasma oxidized LDL levels were seen 7 days after AMI (after 1 day: 14.71.5, 7 days: 21.02.8, 14 days: 20.22.8, 30 days: 18.32.5, 90 days: 16.52.3 U/mL). Plasma oxidized LDL levels 7 days after AMI were significantly correlated with left ventricular end-diastolic volume (1157
mL
;
r
=0.54, P=0.0025) and end-systolic volume (585
mL
;
r
=0.49, P=0.008) 3 months after the AMI. Moreover, they were also correlated with end-diastolic volume index (684 mL/m², r=0.40, P<0.05). However, no correlation was seen between peak plasma oxidized LDL levels and ejection fraction. These findings suggest that oxidative stress may play an important role in the development and progression of left ventricular remodeling after AMI.

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Angiographic Determination of Left and Right Ventricular Volumes and Left Ventricular Mass in Normal Infants and Children

TOMOHARU AKIBA, MITSURU NAKASATO, SATOSHI SATO, ATSUKO KIKAI

The Tohoku Journal of Experimental Medicine
1995年 177 巻 2 号 153-160
発行日: 1995年
公開日: 2006/08/31

DOI https://doi.org/10.1620/tjem.177.153

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AKIBA, T., NAKASATO, M., SATO, S. and KIKAI, A. Angiographic Determination of Left and Right Ventricular Volumes and Left Ventricular Mass in Normal Infants and Children. Tohoku J. Exp. Med., 1995, 177 (2), 153-160-Left and right ventricular (LV and RV) volumes and LV mass were assessed by angiography in 63 infants and children without major cardiovascular abnormalities. LV and RV end-diastolic volumes (EDV) were excellently expressed as a function of body surface area (BSA) with an exponential equation: LVEDV=83.7 (BSA)^{1.29ml
(r
=0.96, p<0.001), RVEDV=87.9 (BSA)^{1.29ml
(r
=0.94, p<0.001). LV ejection fraction (E F) averaged 64±6 ( mean±standard deviation) %, and RVEF 56±5%, each of which was independent of BSA. LV mass was well expressed as a function of BSA with an exponential equation: LV mass=75.4 (BSA)^1.22g (r=0.86, p< 0.001). LV mass/LVEDV was constant, and averaged 0.96±0.22g/ml. Thus, the values of LVEDV, RVEDV, and LV mass in infants and children with various heart diseases may be estimated with reasonable accuracy as a percentage of normal values predicted from their BSA by using the above-proposed exponential equations. The normal values of LVEF, RVEF and LV mass/LVEDV themselves can be compared with these parameters in pediatric patients with heart diseases because of their independence of BSA.-ventricular volumes; ejection fraction; left ventricular mass; angiography; normal infants and children
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B-6. プロスタグランディン類の酵素免疫測定法

林陽子, 矢野智子, 河村純夫, 上田夏生, 大串文隆, 山本尚三, 中村観善

臨床化学シンポジウム
1982年 21 巻 48-51
発行日: 1982/07/15
公開日: 2012/11/27

DOI https://doi.org/10.14921/jscc1971a.21.0_48

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Radioimmunoassay (RIA) is a sensitive, specific and handy method, which has been widely utilized in the determination of prostaglandin (PG) and thromboxane (TX). In consideration of the disadvantage of RIA depending on the use of radioisotope we made attempts to apply enzyme immunoassay (EIA) to the assay of PG and TX, in which enzyme-labeled hapten molecule is used instead of radioactive compound.
The mixed anhydride reaction was performed to conjugate the amino group of β-galactosidase to the carboxyl group of PGF_2α, TXB₂ and 6-keto-PGF_1α, respectively. The enzyme-labeled antigen could be stored at 4° without an appreciable loss of enzyme activity and antigenicity for more than a year.
After competitive immunoreaction with antibody between enzyme-labeled antigen and unlabeled antigen the immunoprecipitate formed was separated by the double antibody method. The enzyme activity of the precipitate was assayed fluorometrically with 4-methylumbelliferyl-β-Dgalactoside as substrate. PGF_2α, TXB₂ and 6-keto PGF_1α could be measured quantitatively in the range of 0.06-30pmol, 0.1-30pmol, 0.14-30pmol, respectively. The sensitivity was comparable to that of RIA. The cross-reactivities with other PGs and their metabolites were less than 1% in most cases.
A column of octadecyl silica was used to extract TXB₂ which was added to human plasma, and the extract was subjected to RIA and EIA. A good correlation was obtained between the amount of added TXB₂ and that of measured TXB₂ (y=1.09x+11.07pmol/
ml
,
r
=0.99) A satisfactory interrelation was also observed between RIA and EIA (y=0.92x+4.64pmol/
ml
,
r
=0.96).
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高速液体クロマトゲラフィーによる血清中Gliclazide濃度測定法の開発と糖尿病患者および肝硬変合併糖尿病患者における体内動態

町支臣成, 寺澤千佳子, 大田博子, 岸田充廣, 堀本重紀, 高科成良

病院薬学
1993年 19 巻 1 号 46-52
発行日: 1993/02/20
公開日: 2011/08/11

DOI https://doi.org/10.5649/jjphcs1975.19.46

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Gliclazide is a hypoglicemic drug that highly binds to plasma proteins, but it is little reported about pharmacokinetics of free gliclazide which has a hypoglicemic action. In this study, we had an aim of establishing the HPLC method to measure free gliclazide and clarifying the effect of some diseases on it. Calibration curves were linear in the range of 0.2-10μg/
ml
(
r
=0.999). The limit of determination was 0.2μg/ml (C. V. 2.8%). Binding of gliclazide was not influenced by FFA under conditions employed and was dependent on bovin serum albumin (BSA) concentrations in vitro. In clinical, area under the curve (AUC) of free gliclazide of NIDDM with liver cirrhosis (n=3) was twice that of NIDDM without complication (n=8).(2.7±0.2 vs 1.3±0.2μg·hr/ml)
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Group-specific Quantification of mcrA genes of Methanogenic Archaea and “Candidatus Methanoperedens” by Digital PCR

Takeshi Watanabe, Atsuya Endo, Rio Hamada, Rina Shinjo, Susumu Asakawa

Microbes and Environments
2025年 40 巻 2 号論文ID: ME24097
発行日: 2025年
公開日: 2025/05/09

DOI https://doi.org/10.1264/jsme2.ME24097

ジャーナルオープンアクセス HTML
電子付録

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Digital PCR is a technique that quantifies target genes based on the absence or presence of the targets in PCR amplicons. The present study examined group-specific probes for the quantification of mcrA genes in six methanogenic archaeal groups and “Candidatus Methanoperedens” by digital PCR with the universal primers ML-f and
ML
-
r
. A digital PCR analysis of paddy field soil detected all the targets, with the dominant and minor groups being Methanomicrobiales and Methanobrevibacter spp., respectively (10⁷ and 10⁴ copies [g dry soil]^–1). This method has the potential to reveal the dynamics of specific methanogenic archaeal groups in the environment.

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排尿機構にかんする検討

第1報正常成人男子の尿流量測定について

八竹直, 秋山隆弘, 門脇照雄, 南光二, 井口正典, 金子茂男, 郡健二郎, 栗田孝

日本泌尿器科學會雑誌
1977年 68 巻 8 号 737-744
発行日: 1977/08/20
公開日: 2010/07/23

DOI https://doi.org/10.5980/jpnjurol1928.68.8_737

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The measurment of urinary flow rate is clearly the most important objective screening test for evaluations of normal or abnormal urodynamics in the lower urinary tract.
The various types of flowmeters have been reported for this purpose. We have used mictiometer 14-F-40 in DISA urological investigation system which has been tested for its accuracy.
In this study 177 normal urinary flow curves in 8 adult males were statistically analysed using 10 parameters in the urinary flow curves.
The results obtained were as follows;
1. Maximum flow rate (MFR) increased propotionally with volumes below 200
ml
(
r
=0.8623, p<0.01), so that it is difficult to determine the normal range of MFR in these volumes. Therefore calculation of voided volume/MFR (caluculated minimal time of micturition) were enhanced. This normal range was calculated as less than 10 seconds. MFR were almost constant with volumes over 200ml (27.7±3.8ml/sec, mean±s. d.).
2. Average flow rate (AFR) increased propotionally with volumes below 250
ml
(
r
=0.8892, p<0.01) as MFR. With volumes over 250ml, AFR were almost constant. The variation of AFR were less pronounced than that of MFR (18.2±2.7ml/sec).
3. Increasing rate of micturition time was larger with volumes over 400ml than below 400ml. On the contrary time to maximum flow (T max.) was shortened with voided volume over 400ml.
4. Other parameters were less significant.
Cases with quite different urinary flow patterns or urinary flow profiles gave same values of parameters. Therefore, it is possible to give wrong interpretation when only one parameter is chosen for evaluation of micturition status. We emphasized that evaluation of urinary flow patterns itself was as important as measurements of parameters such as MFR, AFR, M. T., T. max. etc. for determination of normal or abnormal micturition.
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Microdialysis-MS/MSによるリアルタイム薬物濃度測定法

小林信博, 池田敏彦

薬物動態
1999年 14 巻 supplement 号 132-133
発行日: 1999/09/27
公開日: 2007/03/29

DOI https://doi.org/10.2133/dmpk.14.supplement_132

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Real-time analysis of R-84760 in blood was achieved by a combination of microdialysis (MD) and tandem mass spectrometry (MS/MS). With this technique, the analyte collected in real time from MD is separated by MS/MS, without any subsequent chromatographic separation. The R-84760 concentration was obtained from the plateau part of the ion intensities or the corrected values using an internal standard, after immersing the MD probe into the dialysis solution containing the drug for a definite time. Since contamination of the ion source was prevented by using an organic solvent for the perfused solution, it was possible to establish a stable analysis method. For a MD membrane of length 4 mm, the R-84760 concentration in saline was linear over the range 5 to 541.1 ng/
ml
(
R²
=0.9998). Moreover, the R-84760 concentration in rat whole blood was linear over the range 24.9 to 1868.9 ng/
ml
(
R²
=0.9995). As this method allowed the measurement of free drug concentration in rat blood, this analysis was also able to provide data needed for determining the protein-binding ratio. The protein-binding ratio obtained from the calibration curve in saline and rat whole blood was 87-90%, which was close to the result obtained by another analysis method. The concentration profile of R-84760 in blood as obtained by the MD-MS/MS method correlated well with the concentration profile in plasma, which was simultaneously monitored by LC-MS/MS.
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Development of White Light Emitting Diodes by Multi-layered Red, Green, and Blue Phosphors Excited by Near-ultraviolet Light Emitting Diodes

Takeshi FUKUI, Hiroaki SAKUTA, Kazuya MISHIRO, Tsutomu MIYACHI, Kunihito KAMON, Hideki HAYASHI, Nobutaka NAKAMURA, Yuji UCHIDA, Satoshi KURAI, Tsunemasa TAGUCHI

Journal of Light & Visual Environment
2008年 32 巻 1 号 43-45
発行日: 2008年
公開日: 2008/08/12

DOI https://doi.org/10.2150/jlve.32.43

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Cascade excitation process and cascade excitation loss (absorption loss) have been investigated by bilayer (BL-) LEDs. The cascade excitation loss was suppressed by multi-layered (ML-) LED structure, in which red phosphor is placed at the bottom. We have also developed white LEDs by using a ML-structure of red, green, and blue phosphors for the purpose of reducing the cascade excitation. Luminous flux of ML-white LEDs showed higher value under low correlation color temperature (CCT=2613K) and high color-rendering index (Ra=94) conditions than conventional phosphor mixed-white LEDs. The angular distribution of chromaticity in the
ML
-
R
/G/B white LED was comparable to that in mixed-RGB white LED and was very small.
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吸光光度法による院内製剤セレン注射液の簡易定量試験法

伊藤雅, 嶋田沙織, 横田直之, 土岐浩介, 本間真人

医療薬学
2018年 44 巻 11 号 582-586
発行日: 2018/11/10
公開日: 2019/11/10

DOI https://doi.org/10.5649/jjphcs.44.582

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A simple spectrophotometric assay was developed for quantitative determination of selenium in selenium injection which is a hospital preparation for patients with selenium deficiency. Aliquots of the samples (500 µL) added 20 µL of 2.5 M formic acid and 20 µL of 18.5 mM O-phenylene diamine were incubated for two hours at room temperature. The absorbance was measured in the mixture at 333 nm by using NanoDrop^®, a convenient instrument for spectrophotometric assay. The standard curve for determining selenium concentration was linear in the range of 1.25-30 µg/
mL
(
r
> 0.999). Coefficients of variation for intra- and inter-day assays were 0.3 to 5.5％ and 0.8 to 2.8％, respectively. Assay validation was confirmed by five independent measurements of 50 µg/mL selenium injection in three pharmacists with the accuracy of 0.0 to 1.6％. This assay method was successfully applied to the quantitative determination of selenium in selenium injection.

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INTRAVENOUS LEFT VENTRICULOGRAPHY UTILIZING DIGITAL SUBTRACTION TECHNIQUE

HIROSHI NONOGI, SHIGETAKE SASAYAMA, TSUNETARO SAKURAI, CHUICHI KAWAI, MASATOSHI FUJITA, SHIGERU EIHO, MICHIYOSHI KUWAHARA

JAPANESE CIRCULATION JOURNAL
1984年 48 巻 6 号 559-566
発行日: 1984/06/20
公開日: 2008/04/14

DOI https://doi.org/10.1253/jcj.48.559

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To detect the left ventricular boundary in the intravenous ventriculography, we used a subtraction technique for background suppression. Images containing contrast medium and reference mask images were transfered to a computer through a flying spot scanner and stored on the digital disc. Stored reference mask images were subtracted from the digitized contrast images. The resulting images were then electronically enhanced to extract the left ventricular (LV) image. The LV boundary was delineated with an algorithm we have developed and the volume of the LV cavity was calculated automatically. The validity of this method was compared with data obtained from conventional left ventriculogram (LVG). In 11 patients, values for end-diastolic volume (EDV), end-systolic volume (ESV) and ejection fraction (EF) calculated from the intravenous LVG were correlated closely with those from the conventional LVG (128±38 (SD) vs 133±39
ml
,
r
=0.95; 50±28 vs 53±30
ml
,
r
=0.98; 63±10 vs 62±12%, r=0.96, respectively). Nine patients with valvular regurgitation were followed up serially after valve replacement. EDV index fell significantly after corrective surgery (145±50 to 81±33 ml/m², p<0.02), whereas, EF was affected variably depending upon the preoperative state (58±13 to 61±11%, not significant). Thus, this method is less invasive than conventional LVG and has successfully allowed for sequential determination of ventricular function on an outpatient basis.
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研究心血管造影法による右心室容量計測に関する検討

島崎靖久, 川島康生, 森透, 北村惣一郎, 中埜粛, 大山朝賢, 友国隆, 八木原俊克, 別府慎太郎, 横田侃児

心臓
1979年 11 巻 2 号 163-170
発行日: 1979/02/25
公開日: 2013/05/24

DOI https://doi.org/10.11281/shinzo1969.11.2_163

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心血管造影法による右心室容量測定は,その形態が複雑なために遅れていたが,最近左室容量と同様な方法で求める試みがなされてきている.著者らは可変シリコンゴムを用いて右室腔鋳型を作成し,これの2方向撮影像からarea-legth法およびSimpson法則により鋳型容量を算出した.実際の鋳型の容量は水置換法にて求めた.造影像からの計測値との間にはアンギオカットフィルムではarea-length法で実測値(Vt)=0.828×計測値(Vc)+0.159(ml),Simpson法則でVt=0.856×Vc+2.520(ml),いずれもr=0.995の相関を得た.シネ撮影では鋳型を角度を変えて検討した.どの角度においてもarea-length法でVt=0.759×Vc-0.193(
ml
)(
r
=0.984),Simpso難法則でVt=0.758×Vc+1.520(
ml
)(
r
=0.980)を得た.これらの一方向撮影像だけからでは相関は悪く,2方向撮影像の必要性を示した.右室容量の経時的変化は左室のそれとよく類似していた.
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アロプリノール外用剤の品質に関する検討

皐月由香, 中多陽子, 木村貴, 友井理恵子, 沢辺善之, 山崎勝弘, 田口修三

医薬品情報学
2014年 16 巻 3 号 108-112
発行日: 2014/11/30
公開日: 2014/12/21

DOI https://doi.org/10.11256/jjdi.16.108

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Objective: It is important to ensure the quality of preparation in hospital pharmacies.  Therefore, we evaluated the quantitative method of preparation and the stability of allopurinol for external use in a new dosage form.
Methods: The new dosage form utilized two kinds of ointment, white petrolatum and hirudoid soft ointment, and liquid.  Allopurinol was extracted from these preparations by the liquid-liquid partition method, and assayed by high performance liquid chromatography.  A stability test was conducted for six months in the case of the ointments and one month in the case of the liquid.
Results: Good linearity was obtained, in the range of 30˜670 μg/
mL
(
r²
≥0.999).  The recovery of allopurinol added to the two kinds of ointment was 97.7-102.0%, and the relative standard deviation was less than 3.0%.  It was observed that the quantity remained relatively constant for one month, and increased after three months.
Conclusion: A quantitative method for the preparation of allopurinol in ointment, using white petrolatum and hirudoid soft ointment, was evaluated.  The results confirmed that allopurinol was stable for one month in ointment and liquid.
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新規抗悪性腫瘍薬テモゾロミドのカプセル開封後の溶液中での安定性に関する評価

古俵孝明, 水野知行, 田上裕美, 橋田亨, 矢野育子, 桂敏也, 乾賢一

YAKUGAKU ZASSHI
2009年 129 巻 3 号 353-357
発行日: 2009/03/01
公開日: 2009/03/01

DOI https://doi.org/10.1248/yakushi.129.353

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We evaluated the stability of temozolomide, an alkylating agent, in solutions after opening the capsule. First, we established an analytical method for determination of temozolomide concentration by HPLC. The calibration curve for temozolomide was linear between 0.5-20 μg/
ml
(
r
=0.999). We then evaluated the stability of temozolomide in each buffer solution (pH 2-9) for 30 min. Temozolomide was decomposed pH-dependently between pH 7 and 9, and completely decomposed at pH 9. Temozolomide in several drinking water samples and beverages was decomposed according to their pH values. We also examined the time-dependent degradation of temozolomide in different pH solutions. Temozolomide started to decompose at 5 min in alkaline and neutral solutions, whereas 90% of temozolomide remained intact in acidic solution at 60 min. These results indicate that the stability of temozolomide after opening the capsule is affected by pH of solvents, and temozolomide is almost stable in acidic solutions.

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健康者における血清フエリチン値の検討

高原淑子, 石橋章彦, 佐藤仁政, 佐々木由三, 山下昌次, 安藤裕, 近藤誠, 与那原良夫

医療
1982年 36 巻 3 号 233-238
発行日: 1982/03/20
公開日: 2011/10/19

DOI https://doi.org/10.11261/iryo1946.36.233

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血清フエリチンの各キツトによる正常域を確かめるため, 国立東京第二病院核医学センターで測定値の検討を行つた.
138例の健常者で血清フエリチンの測定を行い, 血液学的および生化学的に厳選した結果, 正常者66例(男46例, 女20例), また59例(男12例, 女47例)は低血鉄症か低フエリチン血症を伴う異常を示した. これらの症例からの血清試料を4種類のRIA Kitで測定した.
結果 1)健康者においても低フエリチン血症はしばしば見られる. 2)キツト間で血清フエリチン値に著明な差が認められたものの, 互いに低い相関々係を示した. 3)男子46例の正常値はスパツク108.1±40.3ng/ml, Gamma Dab 114.1±52.9ng/ml, リアパツク121.5±51.6ng/ml, Riagnost 188.6±60.2ng/mlで, 女子20例の正常値はスパツク39.7±16.3ng/ml, Gamma Dab 37.5±15.7ng/ml, リアパツク56.6±16.4ng/ml, Riagnost 94.8±29.0ng/mlであつた. 以上の結果は各キツトで標準値を決定する際に混乱を招く可能性のあることを示していよう.
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Liquid Chromatography/Mass Spectrometryによる動植物中の還元型グルタチオンの定量

上野清一, 石崎睦雄

衛生化学
1994年 40 巻 1 号 96-100
発行日: 1994/02/28
公開日: 2008/05/30

DOI https://doi.org/10.1248/jhs1956.40.96

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A simple and rapid method for the determination of reduced glutathione (GSH) in plants and biological samples by thermospray high-performance liquid chromatography-mass spectrometry (LC/MS) was studied. After a sample was homogenized in 0.1% trifluoroacetic acid (TFA) in a glass homogenizer with teflon pestle, the homogenate was centrifuged at 10000 rpm for 10 min. The supernatant solution was filtered through disposable syringe filter unit (cellulose acetate, 0.45 μm), and the filtrate was applied for the determination of GSH by selected ion monitoring (SIM) of a protonated molecular ion at m/z 308. Analytical conditions for LC/MS were as follows : column : Shim-pack CLC-ODS (6.0 mm φ×15 cm), mobile phase : 0.05% TFA-acetonitrile (95 : 5 v/v), flow rate : 0.8 ml/min, exit temperature from the vaporizer : 200°C, ion source block temperature : 260°C Under these conditions, GSH in plants and biological samples could be determined with recovery of 95-105%. The coefficient of variation was as low as 5%. The calibration curve was linear between 1 and 100 μg/
ml
(
r
=0.999).
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Simple and Sensitive Method for Measurement of Metoclopramide in Cattle Plasma by LC-MS/MS Using a Multimode Chromatography

Kouko HAMAMOTO, Yasuharu MIZUNO, Masaki KATO, Norio YAMAGISHI, Kazuhisa FURUHAMA

Journal of Veterinary Medical Science
2013年 75 巻 4 号 509-513
発行日: 2013年
公開日: 2013/05/02
[早期公開] 公開日: 2012/11/13

DOI https://doi.org/10.1292/jvms.12-0220

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A quantitative assay method using LC/ESI-MS/MS for simultaneous determination of MCP in cattle plasma was developed and validated. Chromatographic separation was carried out using a multimode column (2 × 150 mm, 3 μm) with gradient elution (0.05% formic acid/methanol with 0.05% formic acid). MCP and levosulpiride (internal standard) were analyzed in the precursor/product ion pair of m/z 300.1/226.9 and 342.0/112.0, respectively. Linear calibration curves were obtained in the range of 2.5–500 ng/
ml
(
R²
>0.999) with a lower limit of quantification of 0.05 ng/ml. Mean recoveries were 96–103%, and the coefficient of variation was less than 6.5%. Plasma MCP concentrations after intravenous administration at 0.4 mg/kg to 12 cattle were determined by the LC-MS/MS method.
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