We isolated two strains of
Chlamydomonas mutants (
RL
-
10
, RL-11) which exhibit only flagellar-type beating and which always swim back-ward. Flagella from these two mutants were similar in that they maintained their flagellar-type beating over a wide range of Ca
2+ concentrations after being demembranated and reactivated in the presence of Mg-ATP. The RL-11 flagellar axoneme changed its amplitude of beating at a Ca
2+ concentration of ca. 10
-6 M, whereas the
RL
-
10
axoneme showed no sensitivity to Ca
2+. In temporary dikaryons of
RL
-
10
(plus mating type) and RL-11 (minus mating type), all four flagella could beat in the normal ciliary mode, evidence that the mutations in the two strains were complementary. Electron microscopy of thin-section specimens showed no structural alterations in the mutant flagella. Several alterations in the SDS gel electrophoretic patterns of the mutant ax-onemes were found.
抄録全体を表示