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  • George W. Lee
    燃料協会誌
    1973年 52 巻 3 号 171-177
    発行日: 1973/03/20
    公開日: 2010/06/28
    ジャーナル フリー
  • Grover Loening
    日本航空学会誌
    1963年 11 巻 119 号 373-380
    発行日: 1963年
    公開日: 2009/05/25
    ジャーナル フリー
  • I.
    F
    . Davies
    Journal of Light & Visual Environment
    1984年 8 巻 1 号 1_9-1_20
    発行日: 1984年
    公開日: 2011/05/20
    ジャーナル フリー
  • Joey
    B
    . Tanney, Leonard J. Hutchison
    Mycoscience
    2012年 53 巻 1 号 31-35
    発行日: 2012年
    公開日: 2023/03/31
    ジャーナル オープンアクセス

    The ability of Climacodon septentrionalis to immobilize and kill a mycophagous nematode (Aphelenchoides sp.) in vitro is described for the first time. Two isolates produced droplets (20–45 μm in diameter) that formed at the apices of tall, stalked, and branching secretory cells (700–

    1
    ,500 μm tall). On 2% modified malt extract agar, nematodes became enveloped in the droplets, which restricted their ability to move and resulted in complete immobilization and death within several hours of contact. The rate of decomposition of the nematodes varied considerably, with most individuals persisting for weeks whereas others were degraded within several days and appeared to be colonized by dense hyphal growth. This study provides the first documentation of a non-agaricoid fungus producing secretory cells that are able to immobilize nematodes.

  • 山本 雅史, Abkenar Asad Asadi, 松本 亮司, 根角 博久, 吉田 俊雄, 國賀 武, 久保 達也, 冨永 茂人
    園芸学会雑誌
    2007年 76 巻 1 号 36-40
    発行日: 2007年
    公開日: 2007/03/06
    ジャーナル フリー
    カンキツ主要12種を用いて染色体のクロモマイシン
    A3
    (CMA) 染色を行った.染色体は CMA(+) バンドの有無および位置から
    7
    種類に区分できた.すなわち,CMA(+) を A: 両端および動原体近傍に有する,
    B
    : 一方の端部と動原体近傍に有する,C: 両端に有する,D: 一方の端部に有する,
    E
    : CMA(+) がない,
    F
    : 動原体近傍に有する,Dst: 付随染色体を有する D 型,である.各種はこれらのうち 2~6 種類の染色体を有し,独自の CMA バンドパターンを示した.C. medica では 2
    B
    +
    8
    D +
    8
    E
    C. limon では
    1
    B
    +
    1
    C +
    8
    D +
    8
    E
    C. aurantifolia では 2
    B
    +
    9
    D +
    7
    E
    C. aurantium では
    1
    A +
    1
    B
    +
    1
    C +
    7
    D +
    8
    E
    C. sinensis では 2
    B
    + 2C +
    7
    D +
    7
    E
    C. maxima では
    3
    A +
    3
    C + 4D +
    8
    E
    C. paradisi では 2A +
    3
    C + 6D +
    7
    E
    C. ichangensis では 2
    B
    + 2C + 12D + 2
    E
    C. latipes では 2A + 5C +
    8
    D +
    3
    E
    C. micrantha では
    1
    B
    + 11D + 4
    E
    + 2Dst,C. macroptera では 2
    B
    +
    1
    C + 11D +
    3
    E
    +
    1
    F
    C. hystrix では
    3
    B
    +
    1
    C +
    8
    D +
    3
    E
    + 2
    F
    +
    1
    Dst であった.
  • EMIL BUCHTA, Dieter Kiessling
    International Symposium on the Chemistry of Natural Products
    1964年 1964 巻 E-8-3
    発行日: 1964/04/12
    公開日: 2017/08/18
    会議録・要旨集 フリー
  • 小林 優, 家坂 貴子, 中野 江身子, 平山 耕一郎
    天然有機化合物討論会講演要旨集
    1989年 31 巻 71/P2-1
    発行日: 1989/09/17
    公開日: 2017/08/18
    会議録・要旨集 フリー
    Six new cembranoids sarcophytol P (
    3
    ), R (4), S (5), K (
    8
    ),
    F
    (11), and T (13) were isolated from the soft coral Sarcophyton glaucum. Sarcophytol P (
    3
    ) was shown to be the 20-hydroxy derivative of the major component sarcophytol A (
    1
    ), and afforded the cyclization product 6 in CHCl_
    3
    at room temperature, in a same way as in
    1
    . Sarcophytols R (4) and S (5) were correlated to
    1
    , by conversion of its
    7
    R,
    8
    R and
    7
    S,
    8
    S epoxide derivatives. Sarcophytol K(
    8
    ) was a 13, 14-dihydroxycembranoid having a
    1
    E
    ,
    3
    Z-diene moiety. The absolute configuration of
    8
    and its
    1
    Z,
    3
    E
    - and
    1
    Z,
    3
    Z-isomers sarcophytol
    B
    (2) and J (10) were determined by circular dichroism study of their bis-p-dimethylaminobenzoate derivatives. Sarcophytols
    F
    (11) and T (13) were
    1
    E
    - and
    1
    E
    ,
    3
    Z-isomers of
    1
    . Compound 11 showed characteristic broadening of the ^
    1
    H-NMR chemical shifts, due to the restricted conformational interconversion. Using the three cembranoids sarcophytols
    F
    (11,
    1
    E
    ,
    3
    E
    ), N (15,
    1
    Z,
    3
    Z) and T (13,
    1
    E
    ,
    3
    Z), spontaneous autoxidation-cyclization, in CHCl_
    3
    ,was examined, in order to compare the stereochemical course of the reaction with that of
    1
    (
    1
    Z,
    3
    E
    ), which affords trans-fused bicyclo[
    9
    .
    3
    .0]tetradecene systems. The
    1
    E
    ,
    3
    Z-isomer 13 gave the same cyclization product 18, as in the reaction of
    1
    , even though it is isomeric at C-
    1
    ,
    3
    . The
    1
    Z,
    3
    Z isomer 15 gave 19, in consequence of the reversed geometry at C-
    3
    of 15. The
    1
    E
    ,
    3
    E
    -isomer (11) gave the bicyclic product
    22
    , having an antipodal fusion as compared with 19. This was confirmed by PCC oxidation of 19 and
    22
    , giving enantiomeric ketones 23 and 24 respectively. The
    1
    (14)-epoxide 26 was shown to be the immediate precursor of
    22
    , and acounted for the inversion of the geometry at C-
    1
    of the cyclization product. Compound 26 is isomeric with the epoxide 17 derived from
    1
    . The epoxide 17 is the postulated precursor in the conversion of 13 to 18. It is noteworthy that the mode of antipodal fusion of the cyclopentane rings, in 18 and
    22
    , was controlled by the chirality of the epoxy rings. The C-14 hydroxyl group participates in the transannular cyclization, but was found not to be the requisite functional group for the reaction. Similar treatment of cembrene C (30), the parent hydrocarbon of
    1
    , also reacted in CHCl_
    3
    giving the bicyclic product 32.
  • Keishi Hata, Fuyuki Sugawara, Naganori Ohisa, Saori Takahashi, Kazuyuki Hori
    Biological and Pharmaceutical Bulletin
    2002年 25 巻 8 号 1040-1044
    発行日: 2002年
    公開日: 2002/08/01
    ジャーナル フリー
    We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MC
    3
    T
    3
    -
    E
    1
    . Sixteen phosphate buffered saline (PBS),
    8
    boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MC
    3
    T
    3
    -
    E
    1
    cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum, and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (
    22E
    ,24R)-ergosta-
    7
    ,
    22
    -diene-
    ,5α,6β-triol (
    1
    ).
    1
    induced ALP activities of MC
    3
    T
    3
    -
    E
    1
    cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MC
    3
    T
    3
    -
    E
    1
    cells by
    1
    , ergosterol (2), ergocalciferol (
    3
    ), cholesta-
    ,5α,6β-triol (4),
    7
    -dehydrocholesterol (5) and cholecalciferol (6) were tested. The enzyme activities of MC
    3
    T
    3
    -
    E
    1
    cells were increased
    3
    .0-fold by 10 μM
    1
    and 2.4-fold by 10 μM 4. However, 2,
    3
    , 5 and 6 did not induce MC
    3
    T
    3
    -
    E
    1
    cell ALP activity at 0.
    1
    —10 μM. These results suggested that the OH groups at C-5 and/or C-6 of
    1
    and 4 played an important role in their differentiation-inducing activities on MC
    3
    T
    3
    -
    E
    1
    cells. Furthermore,
    1
    suppressed induction of MC
    3
    T
    3
    -
    E
    1
    cell apoptosis by serum starvation.
  • 鈴木 敏弘
    日本内分泌学会雑誌
    1960年 36 巻 6 号 1075-1091,925
    発行日: 1960/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    Metabolism of body fluids and electrolytes which related not only to aldosterone alone but to other corticosteroids, for example, glucocorticoids had mineral action. And the simultaneous administration of aldosterone and corticosterone consistently resulted in a greater potassium diuresis than did the aldosterone alone.
    Therefore, body fluids and electrolytes are aummation of many corticosteroids which had biological action, and can't know by the determination of simple corticosteroids.
    The author report on the systemic determination of aldosterone, Comp.
    B
    , Comp.
    E
    , Comp.
    F
    , and the newly separated contaminants.
    Method :
    The 24 hours urine specimen is pooled at 5°C and acidified to pH
    1
    .5 with conc. HCL, then standing 24 hours at 15°C.
    Urine was extracted by chloroform and dried. Extract was treated with Florisil column chromatographies and 4 consecutered and different paper chromatographies with Chloroform-Formamide System, Bush-C System,
    E2B
    System, and Bush-
    B
    5 System, to separated corticosteroid partitions.
    Results :
    1
    . Recoveries of 5 mg/100 ml H2O standard samples after 5 times continued extraction by 0.
    1
    volume chloroform, for Comp.
    B
    , Comp.
    E
    , and DOC is 95%, and Comp.
    F
    is
    83
    %.
    2. Additional chloroform re-extraction, Comp.
    B
    , Comp.
    E
    , and DOG do not appear in the
    7
    th chloroform extraction, but Comp.
    F
    appear in the amount of 60μg in
    8
    th chloroform and
    9
    th extraction is nothing, thus this determination necessitate at least
    8
    consecutered extractions.
    3
    . Comp.
    B
    , Comp.
    E
    , and Comp.
    F
    appear in 25% methanol in chloroform fraction by the use of the Florisil column chromatography is made, and recovery is about 100% in condition of 60 drops/minutes.
    4. Separate Comp. III, Comp. V, Comp. VI, and Comp. VII from aldosterone partition in Neher-Wettstein's method.
    5. New contaminants were discover from Comp.
    E
    and Comp.
    F
    partition after Bush-C System paper chromatography, called Comp.
    E2
    -
    E42
    , Comp.
    F2
    ; and, other conatminants named
    M1
    -
    M8
    Z1
    -
    Z8
    ,
    HC1
    -
    HC3
    ,
    AC1
    and AC2 in Chloroform-Formamide and Bush-C System paper chromatography.
    6. Examination of the purities of Comp.
    B
    , Comp.
    E
    , Comp.
    F
    , and aldosterone partitions is made use of the ultra violet absorption spectrum, and necessitates 4 (C-
    F
    →Bush-C→
    E2B
    →Bush-
    B5
    ), or
    3
    (C-
    F
    E2B
    →Bush-
    B5
    ), paper chromatographies to satisfactorily separate these compounds.
    7
    . The H2SO4 chromogen spectrum of these partition correspond with the standard compounds.
  • Özkan ASLANTAŞ, Ebru Şebnem YILMAZ
    Journal of Veterinary Medical Science
    2017年 79 巻 6 号 1024-1030
    発行日: 2017年
    公開日: 2017/06/16
    [早期公開] 公開日: 2017/04/27
    ジャーナル フリー

    This study aimed to determine the prevalence of fecal carriage of extended spectrum β-lactamase (ESBL) and/or plasmidic AmpC β-lactamase (pAmpC) producing Escherichia coli among dogs (n=428) in Turkey. Polymerase chain reaction (PCR) and sequencing were used to characterize genes encoding β-lactamase and plasmid mediated quinolone resistance (PMQR). Antimicrobial susceptibility testing and PCRs for virulence genes and phylogenetic groups were also performed. Cefotaxime resistant

    E
    . coli isolates were detected in 95 (
    22
    .2%) of the swab samples. Sequencing analysis results showed occurrence of various β-lactamase genes: blaCTX-M-15 (62), blaTEM-
    1b
    (42), blaCMY-2 (
    22
    ), blaCTX-M-
    3
    (16), blaCTX-M-
    1
    (15), blaOXA-
    1
    (
    9
    ) and blaSHV-12 (
    3
    ) alone or in combination. The most frequently encountered phylogenetic group was group
    A1
    (35.
    8
    %), followed by group D2 (
    22
    .
    1
    %),
    B
    1
    (15.
    8
    %),
    D1
    (
    9
    .5%), A0 (
    7
    .4%),
    B22
    (5.
    3
    %) and
    B23
    (4.2%), respectively. PMQR genes, aac(6’)-Ib-cr,
    qnrS1
    and qnrB10 were detected in 25.
    3
    , 10.5 and
    1
    .
    1
    % of the isolates, respectively. While all isolates were susceptible to imipenem and amikacin, resistance rates to non-β-lactam antibiotics ranged from 20.0% for tobramycin to 56.
    8
    % for tetracycline. The virulence genes were only detected in 34 (36.2%) of the isolates and this isolates carried single or various combination of virulence genes of iucD, papC, papE,
    f17a
    -A and eaeA. Four isolates were identified as human virulent pandemic CTX-M-15 producing
    E
    . coli
    clone O25
    b
    :ST131/
    B
    2. To the best of our knowledge, this is the first study to show fecal carriage of ESBL/pAmpC type β-lactamase producing
    E
    . coli
    isolates among dogs in Turkey.

  • 林 雄二, 松本 武
    天然有機化合物討論会講演要旨集
    1981年 24 巻 42
    発行日: 1981/09/10
    公開日: 2017/08/18
    会議録・要旨集 フリー
    Reactions of nor-diterpenoid dilactones isolated from Podocarpus plants have been investigated. The
    7
    :
    8
    ,
    9
    : 11-dienolide type [C] dilactones, the least available but biologically the most active members, are mainly derived from naturally abundant dilactone components of α-pyrone type [A] and
    7
    :
    8
    -epoxy-
    9
    : 11-enolide type [
    B
    ] for total-synthetic and biological purposes. From nagilactone
    E
    (5), for example, a combined sequence of hydrogenolysis of
    7
    :
    8
    -epoxide group and dehydration gives nagilactone
    F
    (
    7
    ),
    3
    β-hydroxynagilactone
    F
    (
    8
    ) and 2:
    3
    -dehydronagilactone
    F
    (19), which have been found from natural sources. From nagilactone A (
    1
    ), photo-hydration and concomitant double bond migration of its acetate results in a lactol (
    22
    ), which is reduced to
    1
    β-hydroxynagilactone
    F
    (
    9
    ), another natural type [C] dilactone. Derivation of the type [A] or [
    B
    ] from others is not successful. Some attempts for conversion of the ring A functional groups have also been studied.
  • M. L. GHOSH
    Journal of Physics of the Earth
    1972年 20 巻 2 号 165-186
    発行日: 1972年
    公開日: 2009/04/30
    ジャーナル フリー
    The possibility of attenuation of microseismic waves due to the presence of an upward folding of the ocean bottom into the liquid is discussed in this paper. The upward folding of the ocean bottom has been idealized by means of an irregularity in the form of a rectangle intruding into the liquid and the evaluation of the displacement component at any point of the solid medium has been carried out in two stages. First, the change in the incident mode of the surface wave due to the presence of the rectangular irregularity has been determined, and second, the subsequent change of the resulting displacement components due to the termination of the liquid layer and the step change in elevation of the solid medium at the continental margin has been discovered. Finally, numerical calculation has been made to determine the attenuation of the microseismic waves of different periods due to the presence of the rectangular irregularity at the ocean bottom.
  • Mamoru KOH, Tetsuo IWANAGA, Masahiro HATANAKA, Akio NAKANO, Kazuyuki MORIHARA, Kaoru TAKEMURA
    Bioscience, Biotechnology, and Biochemistry
    2000年 64 巻 4 号 858-861
    発行日: 2000年
    公開日: 2005/03/01
    ジャーナル フリー
      The distribution of sarcophytol-A in the Sarcophyton genus was investigated in seven samples belonging to S. glaucum (
    3
    samples), S. infundibulifurme (2 samples), S. crassocaule (
    1
    sample) and S. trocheliophorum (
    1
    sample) that were collected on Ishigaki Island in Okinawa Prefecture. Sarcophytol-A was present in one sample each of S. glaucum and S. infundibulifurme. This study indicates that the composition of cembranoids in the Sarcophyton genus is not related with the respective species, but with the individual samples collected.
  • 板倉 智敏, 五藤 精知, 藤原 義昭
    日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
    1973年 35 巻 4 号 289-297_5
    発行日: 1973/08/25
    公開日: 2008/02/13
    ジャーナル フリー
    All the birds examined were also associated with bone dysplasia (art alczaormal develop-ment of bone tissue) in the diaphyseal cortex of the leg bones, especially of bones affectedwith dyschondroplasia. This result is very significant in order to clarifyz tlais disease entity.1970:J14, k -
    1
    }
    7
    )
    7
    , M77 ) i? ht /, ( K )
    7
    (C # L"C, MB @ (
    7
    ) t, If!TB& U "C, Jff Dyschondroplasia (
    1
    *t%
    7
    fi!dW) ().g:#
    f
    [!:.fLlt! K?
    3
    o, [
    1
    : (
    7
    )M;tC, rff.?U/:cD
    8
    , bb=
    1
    lC#li6*H(DX15RI!!D& t, r, ccl)Efff
    7
    flr.#.??t"ut:2.?T<
    3
    .
    1
    .?l?
    F
    ?/?t:>"A??
    1
    !? >
    E
    >?TMOll?, lI]%G (
    f
    -srRM) -ts;5;
    e
    .*BI) *M[t fL 54 5 t:.;*.{
    1
    *I[l?(), t@Yllll*fJ?H@(
    7
    ) %?Jfipj@ (Dyschondroplasia) p, }, (,
    1
    lTi{-X, WIKIM itt{
    b
    =
    b
    t D"tl%tff{tO 1111IH(-UP!z
    8
    U-ii) >ES- -)%Wi*fd?
    1
    J*[lEj: >11"i6ifI".*"H*Tl*&Z") #)/2?-"k l
    E
    B
    - vich ) J&it(Vh
    3
    iO"= >If-ffi"!
    1
    lYl*I4")Ji/l![40111!>D
    9
    *
    F
    !)<Y14M)?A"!>CL(Il], k
    7
    .Cl
    8
    T"ltTt*flB
    1
    ? & $4U [-" C U" L % w, Zl
    1
    *.IlBl tI
    1
    J, FA(y L-x") LVfAK t: >
    3
    llltPJ4IiI " } ) t- ! Al j: >kP11liR6, &ffl(C5NWi Z
    3
    lk(C#L" Ct ?
    f
    !4M2"C- fL#, -()%
    E
    .IWMd
    1
    .XI
    1
    *I
    1
    EPW(tit ff
    1
    lR)
  • 佐藤 敬之輔
    デザイン学研究
    1970年 1970 巻 11 号 45-55
    発行日: 1970/03/30
    公開日: 2017/07/25
    ジャーナル フリー
    [
    1
    ] The purpose of this reserch is to make clear what type of design can give good effects to win the high readership score. At the begining I had to know what factors decide the readership score, and how to change the quality of the desing into numerical value. I have used the method of multi-dimentional analysis with the help of a computer, and got the estimation formula, with
    7
    kinds of factors, as follows ; A^^〜=
    B
    +C+D+K+aE+bF+cG. A^^〜=estimated values of the readership score.
    1
    )
    B
    , C, D, K : these four factors are given as category values (so we call them); each factor is classified into several categories.
    B
    =week day, classified into
    7
    categories. C=pages of a newspaper, which are characterized by the kinds of articles, news, and the other reading matters, classified into
    22
    categories. D=kinds of goods and trades of the advertizement, classified into 14 catedories. K=constants, given to each 13 surveys. 2)
    E
    ,
    F
    , G : these factors are given as numerical values. G=area of advertizement, measured by the unit of column in the whole page length, being classified into 6 kinds of area (2.5,
    3
    .5, 5,
    7
    , 10, 15) and 15 column means a whole page, about 2,000cm^2.
    E
    ,
    F
    are the marks obtained in the design of advertizements.
    E
    =sum of the marks obtained in each design element on the advertizement.
    F
    =the marks obtained on the whole effect of the design.
    3
    ) a,
    b
    , c : coefficients Using the above formula with the category values and the coefficients, we can obtain the naked design effect from the actual value A of readership score: aE+bF=A-(
    B
    +C+D+K+cG). In this research, I used
    1
    ,829 data of readership score from 1960 to 1968, being obtained by 13 survevs, spring and autumn twice a year. The sample of size each survey was
    3
    ,541, 2,251, or 200 in the other 11 surveys. Multiple correlation coefficient between the estimated value A^^〜 and the actual value A is 0.951. The table 2^* shows the contribution indexes of each factor by
    3
    kinds of expression-(
    1
    ) Range : the absolute difference between the maximum and the minimum category values, (2) Standard deviations of the category values, (
    3
    ) Partial correlation coefficient : the relationship between the actual values A and each factors. *see the table 2 in the thesis in Japanese. [2] The important point was in the determination of the values of
    E
    ,
    F
    .
    E
    is the sum of the marks obtained in each design element, classified into four kinds :
    E
    =
    E
    _
    1
    +
    E
    _2+
    E
    _
    3
    +
    E
    _4. design elements [table]
    E
    _
    1
    ,
    E
    _2,
    E
    _
    3
    ,
    E
    _4, these values have the 5 grades, as 0,
    1
    , 2,
    3
    , 4 and 0 is given to the design that has no attractive effect or no applicable element. And
    E
    obtain the marks of 0 to
    9
    grades as the sum of them. The values of
    F
    have also 5 grades, 0 to 4. The principles to determine the values of
    E
    ,
    F
    are as follows : a) The marks obtained of
    E
    ,
    F
    must be the relative values among the each survey, and at the same time, they must have constancy within the same survey-the same elements of design must win the same marks obtained.
    b
    ) They must be determined as to win the highest multiple correlation coefficient, when they are put into the estimation formula. c) They must be reasonable. In order to justify them, we must carry many researches on the actual condition. The frequency of accurence of each grade of
    E
    ,
    F
    values, as the table 5-4, and 5-5 in the thesis in Japanese.
  • Hin-chung Wong, Chia-Yun Ho, Li-Ping Kuo, Tien-Kuei Wang, Chi-Lung Lee, Daniel Yang-Chih Shih
    MICROBIOLOGY and IMMUNOLOGY
    1999年 43 巻 7 号 631-636
    発行日: 1999年
    公開日: 2008/03/17
    ジャーナル フリー
    Vibrio parahaemolyticus is a prevalent food-borne pathogen in Taiwan, Japan and other Asian countries. This work presents a novel ribotyping method for the molecular epidemiological examination of this pathogen. Genomic DNA was fragmented by HindIII digestion and hybridized with cDNA probe for Escherichia coli 16S and 23S RNA genes. A total of 121 isolates obtained from outbreaks during 1992 and 1994 in Taiwan were characterized by this ribotyping method. Four to seventeen restricted fragments were visualized in these isolates. After hierarchical cluster analysis, these isolates were grouped into thirty different ribotypes. In addition, A
    3
    , A
    7
    ,
    E
    3
    and
    F
    1
    were the major ribotypes, consisting of
    22
    .
    3
    , 13.2,
    9
    .
    1
    , and
    8
    .
    3
    % of the isolates, respectively. A,
    E
    ,
    F
    , G and
    B
    were the major groups, consisting of 46.2, 14.0,
    9
    .
    1
    , 6.
    7
    , and 6.
    7
    % of the isolates, respectively. The discriminatory ability of this ribotyping method, as determined by Simpson's index of diversity, was 0.93, which closely resembled that of a previously reported pulsed-field gel electrophoresis method.
  • 松藤 寛, ガン エン, 千野 誠, 合田 幸広, 豊田 正武, 武田 明治
    日本食品化学学会誌
    2002年 9 巻 3 号 107-113
    発行日: 2002/12/30
    公開日: 2017/12/01
    ジャーナル フリー
    市販食用緑色
    3
    号(主色素:HSBA-(m-EBASA)(m-EBASA)、m,m-G-
    3
    )に含まれる
    3
    付随色素(Sub-A、
    B
    E
    )を精製単離し、MSおよびNMRを用いて構造決定した。Sub-Aは主色素のエチル基の一つが脱離した化合物HSBA-(m-BASA)(m-EBASA)、Sub-
    B
    はp,p-G-
    3
    、Sub-
    E
    はo,p-G-
    3
    であった。以前報告した付随色素(Sub-C、
    F
    、G、H)を含め、
    7
    つの付随色素の市販品(
    1
    社4製品)中の含量を測定したところ、色素中にSub-Aは0.45〜
    1
    .20%、Sub-
    B
    は0.19〜2.60%、Sub-Cは
    8
    .49〜27.0%、Sub-
    E
    は0.05〜
    1
    .52%、Sub-
    F
    1
    .15〜2.37%、Sub-Gは0.05〜0.
    22
    %、Sub-HはND〜0.24%含まれていた。
  • 広田 鋼蔵, 三道 喜一郎, 畑田 元義
    日本化學雜誌
    1960年 81 巻 1 号 29-33
    発行日: 1960/01/10
    公開日: 2011/05/30
    ジャーナル フリー
    エチレングリコールにγ-線照射を行なうと, (a)水素, (
    b
    )メタン, (c)-酸化炭素, (d)エタン, (
    e
    )アセトアルデヒド, (
    f
    )メタノール, (g)エタノール, (h)酢酸, (i)酢酸ヱチル, (j)アセトアルデヒドエチレンアセタール,(k)グリコールモノアセテートおよび(l)水が生成する。これら生成物のG値は,吸収エネルギーとともに,(
    f
    )・(
    e
    )・(h)は不変, (
    b
    )・(d)・(g)・(i)は増加,(a)・(j)・(l)は減少する。また
    1
    .5×1021ev/ml のエネルギー吸収の際の,それらのG値はつぎのとおりである。G(a)=
    1
    .
    9
    , G(
    b
    )=
    9
    .
    7
    ×10-2, G(c)=
    1
    .5×10-2, G(d)=5.
    8
    ×10-2, G(
    e
    )=4.0, G(
    f
    )=
    1
    .
    7
    , G(g)=2.5, G(h)=
    1
    .
    1
    , G(i)=5.
    7
    , G(j)=48, G(l)=
    83
    これら生成物のうち, (a)・(
    b
    )・(
    e
    )・(
    f
    )は
    1
    次生成物で, 他は2次生成物と推論される。
  • 長谷川 親太郎
    The Keio Journal of Medicine
    1992年 41 巻 3 号 146-153
    発行日: 1992年
    公開日: 2009/03/27
    ジャーナル フリー
    Tumor-infiltrating lymphocytes (TIL) were tested for cytotoxicity against autologous tumor cells in a study utilizing a chemically induced cancer ofthe bladder (transitional cell carcinoma),
    BC
    -47, in inbred ACI/N rats. From tumors grown after subcutaneous implantation of
    BC
    -47 in the rats TIL were separated by density gradient centrifugation and incubated in plastic dishes for separation of non-adherent from adherent cells. The non-adherent cells were further fractionated into T and
    B
    cells by the panning method using anti-rat
    F
    (ab')2 antibody. The cell fractions were each added to
    BC
    -47 in culture to be assessed for antitumor effect by the crystal violet dye exclusion method and
    3
    H-thymidine incorporation inhibition assay. Peripheral blood mononuclear cells (PBMC) were also tested as described above. TIL expressed significantly higher cytotoxicity against
    BC
    -47 with the mean % cytotoxicity of 56.6±5.6% and 87.5±
    7
    .
    1
    % at
    E
    /T ratios of 10:
    1
    and 20:
    1
    , respectively, as compared to PBMC(
    9
    .
    9
    ±5.0% at
    E
    /T 10:
    1
    ) (P<0.001). The adherent cells,
    B
    and T cell fractions showed respective % cytotoxicity of 92.4±2.
    8
    %, 57.
    9
    ±10.6% and
    9
    .
    9
    ±
    7
    .
    8
    % at an
    E
    /T ratio of 10:
    1
    . TIL pretreated with IFN or rIL-2 for 24or 48 hours did not exhibit any noticeably enhanced antitumor activity at an
    E
    /T ratio of 5:
    1
    . Prevention of direct contact of
    BC
    -47 cells and TIL by an interposed Millipore membrane (0.45μm) resulted in an unequivocal reduction of antitumor effect. This finding clearly indicates that the direct contact with
    BC
    -47cells and TIL is necessary for TIL to manifest their cytotoxicity against the autologous tumor cells, suggesting that TIL exhibit autologous tumor killing activity through cell-mediated cytotoxicity.
  • 増大する資源
    E
    .
    F
    . Boswell
    紙パ技協誌
    1998年 52 巻 7 号 885-889
    発行日: 1998/07/01
    公開日: 2009/11/19
    ジャーナル フリー
    The world is in the midst of a huge fundamental shift in fibre supply, demand balances, and policy changes. Governments around the world are introducing policy changes which influence the supply demand balance as the politicians' thoughts are uttered. Government agencies and their bureaucrats are imposing changes on industry even before the legislation is passed by the government in question.
    This ad hoc change process will continue for a time to come until the countries in question show a tough-mindedness with regard to their land use policies. Policies affecting forest areas such as protection areas, Native land claims, the protection of biodiversity and a true goal of sustainable development ; which addresses the combined issues of cultural, environmental, social and economic matters.
    Eventually all of these difficult issues will reach essential closure. Then there will be great new opportunities for investment to meet the growing demand for forest products ; leading to a strengthening competitive position in the more traditional producing areas such as Canada.
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