Seven hundred fifty mg of cholesterol were fed daily to 32 patients of the ischemic heart disease (IHD) for weeks. Cholesterol amounts in VLDL, LDL, HDL, and HDL₃ were estimated on the 0th, th and 14th days of cholesterol load. Plasma apoprotein A-I, A-II, , C-II, levels were also measured in 16 from 32 IHD subjects. Apoprotein levels were estimated by a method of single radial immunodiffusion (SRID). Before cholesterol administration, cholesterol amounts in VLDL, LDL, HDL, and HDL₃ were 15.±.mg/dl (mean±SD), 150.±58.1mg/dl 43.±.6mg/dl, 16.±.mg/dl and 24.±6.3mg/dl respectively. Plasma apoprotein A-I, A-II, , C-II and levels were .±.0 mg/dl, 15.1±.mg/dl, 93.±36.0mg/dl, 3.83±1.00mg/dl and .29±1.36mg/dl respectively. After weeks' cholesterol feeding, all plasma lipoprotein cholesterol and apoprotein levels did not change significantly. Correlation coefficients between plasma apoprotein and lipoprotein cholesterol levels were calculated. Apoprotein A-I and A-II correlated with HDL-C (r=0.348), -C (r=0.612) and HDL₃ (r=0.569). Apoprotein correlated with total cholesterol (TC) (r=0.610), VLDL-C (r=0.341), LDL-C (r=0.726), HDL-C (r=0.432) and -C (r=0.465). Apoprotein C-II correlated with TC (r=0.765), TG (r=0.679), VLDL-C (r=0.651), LDL-C (r=0.461) and -C (r=0.326). Apoprotein correlated with TC (r=0.580), TG (r=0.575) and VLDL-C (r=0.666). Interapoprotein's correlations were also calculated. Apoprotein A-I correlated with apoprotein A-II (r=0.468). Apoprotein correlated with apoprotein C-II (r=0.393). Apoprotein C-II correlated with apoprotein (r=0.393) and (r=0.549). Apoprotein only correlated with apoprotein C-II (r=0.549).

抄録全体を表示

1) P. aeruginosa has been consistently isolated from 26. to 42.% of the patients with urinary tract infections in the urological ward of Okayama University Hospital for the past years. 232 (.3%) out of 1042 hospitalized patients were with urinary tract infections with P. aeruginosa for past years. Of these, 149 (64.%) were thought to be the catheter induced urinary tract infections. The mode of spreading of this organism was epidemiologically investigated.
) The following three laboratory assays were used for identification of P. aeruginosa isolated from clinical and environmental strains; serological typing, antibiotic sensitivity and protease and elastase production. Serological typing with 13 immunodiagnostic sera (Toshiba Chemical Industry) was performed by slide agglutination method. Protease and elastase production were detected by the method described by Goto et al.
In combination with serological typing and antibiograms, protease and elastase production offers the finest means of differentiating the strains of P. aeruginosa.
3) Of 136 clinical strains of P. aeruginosa, 89.% were serotypable strains. Serotype K was the most prevalent (36.0%), followed by serotype (28.%), F (10.3%) and A (.%). Most of serotype F and which were concurrently isolated from patients in 1976 had the same patterns of antibiogram and production of protease and elastase. It was strongly suggested that these patients were nosocomially cross-infected with the P. aeruginosa from the catheterization or through instrumentation.
) Of 354 environmental specimens, 78 P. aeruginosa (.%) were isolated, which were frequently isolated from floor mops and sink-drains. Of these strains, .% were serotypable strains. Serotype was the most prevalent (42.3%), followed by serotype K (24.%) and (12.%). The same strains were seldom detected at the same sources successively, but different types of P. aeruginosa were usually detected. Among the serotypes , F and K, 18 strains (23.1%) were identical with the clinical strains. All of these were found during or after the clinical outbreaks of P. aeruginosa infections. There was no environmental strains that caused clinical infections. The other 60 strains (76.%) were different from the clinical strains, and present only transiently in the hospital environments.
) Both clinical and environmental strains similary produced protease and elastase at 70 to 80%, hemolysin at 100%. Only 10 out of 37 environmental strains (27.0%) were found to produced exotoxin.

抄録全体を表示