コイ筋原繊維加熱変性の敏感な指標としてのミオシン抽出性の低下
1993 年 59 巻 3 号 p. 515-518
詳細
1993 年 59 巻 3 号 p. 515-518
Myosin extractability from thermally treated carp myofibrils (Mf) was measured as a possible indicator for Mf denaturation. Mf thermally treated at 40°C in 0.1M KCl, 20mM Tris-HCl (pH 7.5) was dissolved in 0.5M KCl, and salted out with ammonium sulfate at 40% saturation in the presence of 5mM ATP-Mg. The amounts of myosin recovered in the supernatant decreased rapidly (within 15min), and the rate was much greater than the inactivation rate of its Ca-ATPase. Nevertheless, myosin obtained in the supernatant exhibited an identical Ca-ATPase activity regardless of the thermal treatment period of Mf, and this myosin was proved to be monomeric by Sepharose CL-4B gel filtration. Therefore, myosin extractability could be used as a sensitive indicator for Mf denaturation.
