抄録
The aim of this work was to look for the stress protein P33, during long-term growth of human EUE cells in a hypertonic medium, with a polyclonal antibody against P33 produced in rabbit, and detectable in cells by both light and electron microscopy, using fluorescein-, peroxidase-anti-peroxidase- and gold-indirect immunolabelling. P33 was found to be mostly located in the cytoplasm, without apparent association with subcellular organelles; a slight, but significant, labelling was also observed in the nucleus. Dual parameter flow cytometric measurements of fluorescein-immunolabelled P33 and DNA content (after propidium iodide staining) showed that the amount of P33 increased progressively with increasing times of growth in a hypertonic medium in all phases of the cycle. Therefore there is no direct relationship between the increase of the P33 and the exit of cells from the cycling compartment that occurs in a hypertonic environment.
