Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Studies on Osmophilic Yeasts
Part XI. Various Factors Affecting on Polyalcohol Production by Pichia miso
Hiroshi ONISHINarimasa SAITOIkunori KOSHIYAMA
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1961 年 25 巻 2 号 p. 124-130

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Various conditions were studied which gave influences on polyalcohol production byPichia miso.Pichia misogave an excellent yield of polyalcohol showing good growth in the vitamin-free medium. Unlike Zygosaccharomyceshigh concentrations of phosphate such as 2% as KH2PO4in the medium showed no detrimental effect on polyalcohol production. Remarkable reduction in polyalcohol yield was observed in the medium of high concentration of nitrogen sources, among which yeast extract showed the most striking effect. Polyalcohol fermentation was provoked in the medium of 0.1% yeast extract while the metabolic activity distinctly converted to ethanol fermentation when the organisms were incubated in the medium of 4.0% yeast extract. The fact that a large amount of ethanol more than 6% in the medium was produced aerobically by genusPichiaof oxidative dissimilation type seemed to be very interesting and noticeable. For the purpose of industrial production, it was shown that polyalcohol production in jar-fermenter scale was achieved with as good yield as that in shaking flask culture.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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