Thiosulfate was oxidized to tetrathionate and an unknown oxidized sulfur compound. The activity was found in the particulate fraction (a precipitate obtained by centrifuging (at 90, 000 ×g for 60min) the supernatant of centrifugation at 10, 000 ×g for 30min). Treatment with deoxycholate caused inactivation of the enzyme. Considering from these points and electronmicrograph, the enzyme would be located on the cell membrane.
