Studies on the Fermentative Production of Purine Derivatives

Part I. Derivation of Guanosine and Inosine-Producing Mutants from a Bacillus Strain

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By the application of various genetical techniques, a number of guanosine and inosineproducing mutants were obtained from a Bacillus strain. The indispensable genetic characters of the mutants concerning with guanosine productivity were adenine requirement, lack of GMP-reductase and mutation to adenine and adenosine resistance from adenine and adenosine sensitiveness. Main products of these mutants were guanosine and inosine. Their culture broths also contained hypoxanthine, xanthine and sometimes u little guanine and xanthosine. Furthermore a few unknown nucleosides were also detected. The yields of guanosine were about 5mg/ml in 4-day culture broths.