Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Regular Articles
Preventive Effect of 7,8-Dihydroxyflavone against Oxidative Stress Induced Genotoxicity
Rui ZhangKyoung Ah KangMei Jing PiaoDong Ok KoZhi Hong WangWeon Young ChangHo Jin YouIn Kyung LeeBum Joon KimSam Sik KangJin Won Hyun
ジャーナル フリー

2009 年 32 巻 2 号 p. 166-171


We elucidated the protective effect of 7,8-dihydroxyflavone against hydrogen peroxide (H2O2)-induced DNA damage. We found that 7,8-dihydroxyflavone scavenges 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). 7,8-Dihydroxyflavone with antioxidant effect prevented the H2O2-induced cellular DNA damage, as evidenced by comet tail, 8-hydroxy-2′-deoxyguanosine (8-OHdG) content, and phospho-histone H2A.X protein expression. Hence, 7,8-dihydroxyflavone was shown to protect cell via the inhibition of apoptosis induced by H2O2. This was substantiated by decreased apoptotic nuclear fragmentation, decreased sub-G1 cell population, and decreased DNA fragmentation. Furthermore, 7,8-dihydroxyflavone activated the protein kinase B (PKB, Akt) signal pathway, which is a major survival signal pathway. In addition, LY294002, which is phosphatidylinositol 3 kinase (PI3K, upstream of Akt) inhibitor, attenuated the protective effect of 7,8-dihydroxyflavone against H2O2-induced cell damage. In conclusion, 7,8-dihydroxyflavone was shown to possess cytoprotective properties against oxidative stress by scavenging intracellular ROS and enhancing Akt activity.

© 2009 The Pharmaceutical Society of Japan
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