- J-STAGEトップ
- /
- 日本畜産学会報
- /
- 65 巻 (1994) 5 号
- /
- 書誌
31P NMR法によるヤギ骨格筋の死後変化の非破壊計測
東 泰好, 河合 文雄, 金森 正雄, 眞鍋 昇, 宮本 元
著者情報
-
東 泰好
(財)環境科学総合研究所
-
河合 文雄
(財)環境科学総合研究所
-
金森 正雄
(財)環境科学総合研究所
-
眞鍋 昇
京都大学農学部
-
宮本 元
京都大学農学部
ジャーナル
フリー
1994 年 65 巻 5 号 p. 416-422
詳細
- 発行日: 1994/05/25 受付日: 1993/10/01 J-STAGE公開日: 2008/03/10 受理日: - 早期公開日: - 改訂日: -
-
訂正情報
訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報 訂正内容: Wrong : 1) AZUMA, Y., N. MANABE, F. KAWAI, M. KANAMORI and H. MIYAMOTO. Phosphorus-31 nuclear magnetic resonance study of energy metabolism in intact slow- and fast-twitch muscles of rat. J. Anim. Sci., 72: 103-108. 1994.
2) BARANY, M. and T. GLONEK, Phosphorus-31 nuclear magnetic resonance of contractile systems. Methods Enzymol., 85: 624-676. 1982.
3) BARANY, M., I. M. SIEGEL, P. N. VENKATASUBRAMANIAN, E. MOK and A. C. WILBUR, Human leg neuromuscular diseases: P-31 MR spectroscopy. Radiology, 172: 503-508. 1989.
4) BENDALL, J. R., The effect of pre-treatment of pigs with curare on the post-mortem rate of pH fall and onset of rigor mortis in the musculature. J. Sci. Food Agric., 17: 333-338. 1966.
5) BENDALL, J. R., Post-mortem changes in muscle. in The structure and function of muscle (BOURNE, G. H., ed.). 243-309. Academic Press. New York. 1973.
6) BURT, C. T., T. GLONEK and M. BARANY, Phosphorus-31 nuclear magnetic resonance detection of unexpected phosphodiesters in muscle. Biochemistry, 15: 4850-4853. 1976.
7) CASSENS, R. G., D. N. MARPLE and G. EIKELENBOOM, Animal physiology and meat quality. Adv. Food Res., 21: 71-155. 1975.
8) GREASER, M. L., Conversion of muscle to meat. in Muscle as Food (BECHTEL, P. J., ed.). 37-102. Academic Press. Orlando. 1986.
9) HOCHACHKA, P. W. and T. P. MOMMSEN, Protons and anaerobiosis. Science, 219: 1391-1397. 1983.
10) HoULT, D. I., S. J. W. BUSBY, D. G. GADIAN, G. K. RADDA, R. E. RICHARDS and P. J. SEELEY, Observation of tissue metabolites using 31P nuclear magnetic resonance. Nature, 252: 285-287. 1974.
11) JORGENSEN, L. and H. GRASDALEN, 31P-NMR studies of phosphate metabolites in intact red and white swimming muscles of cod (Gadus Morhua L.). Comp. Biochem. Physiol., 84B: 447-450. 1986.
12) KASTENSCHMIDT, L. L, W. G. HOEKSTRA and E. J. BRISKEY, Glycolytic intermediate and cofactors in "fast- and slow-glycolyzing" muscles of the pig. J. Food Sci., 33: 151-158. 1968.
13) MANABE, N., T. ISHIBASHI and H. MIYAMOTO, Slow -twitch myofibers are observed in masseter muscle of the hypothalamic obese rat. Anim. Sci. Technol. (Jpn.), 64: 440-447. 1993.
14) MEYER, R. A., T. R. BROWN and M. J. KUSHMERICK. Phosphorus nuclear magnetic resonance of fast- and slow-twitch muscle. Am. J. Physiol., 248: C279-287. 1985.
15) MOON, R. B. and J. H. RICHARDS, Determination of intracellular pH by 31P magnetic resonance. J. Biol. Chem., 248: 7276-7278. 1973.
16) RENOU, J. P., P. CANIONI, P. GATELIER, C. VALIN and P. J. COZZONE, Phosphorus-31 nuclear magnetic resonance study of post mortem catabolism and intracellular pH in intact excised rabbit muscle. Biochimie, 68: 543-554. 1986.
17) SATRUSTEGUI, J., H. BERKOWITZ, B. BODEN, E. DONLON, A. MCLOUGHLIN, J. MARIS, R. WARNELL and B. CHANCE, An in vivo phosphorus nuclear magnetic resonance study of the variations with age in the phosphodiesters' content of humam muscle. Mechanism Ageing Development, 42: 105-114. 1988.
18) SCHMIDT, G., L. HECHT, P. FALLOT, L. GREENBAUM and S. J. THANNHAUSER, The amounts of glycerylphosphorylcholine in some mammalian tissues. J. Biol. Chem., 197: 601-609. 1952.
19) SEELEY, P. J., S. J. M. BUSBY, D. G. GADIAN, G. K. RADDA and R. E. RICHARDS, A new approach to metabolite compartmentation in muscle. Biochem. Soc. Trans., 4: 62-64. 1976.
20) TARRANT, P. J. V., P. V. J. HEGARTY and J. V. MCLOUGHLIN, A study of the high energy phosphates and anaerobic glycolysis in the red and white fibres of porcine semitendinosus muscle. Proc. Royal Ir. Acad., 72 (C): 229-251. 1972.
21) UHRIN, P. and T. LIPTAJ, 31P NMR study of phosphorus metabolites in fast and slow muscles. Int. J. Biochem., 22: 1133-1138. 1990.
22) UHRIN, P. and T. LIPTA,1, 31P NMR study of postmortem metabolism in porcine and bovine muscles. Gen. Physiol. Biophys., 10: 83-93. 1991.
Right : 1) AZUMA, Y., N. MANABE, F. KAWAI, M. KANAMORI and H. MIYAMOTO. Phosphorus-31 nuclear magnetic resonance study of energy metabolism in intact slow- and fast-twitch muscles of rat. J. Anim. Sci., 72: 103-108. 1994.
2) BARANY, M. and T. GLONEK, Phosphorus-31 nuclear magnetic resonance of contractile systems. Methods Enzymol., 85: 624-676. 1982.
3) BARANY, M., I. M. SIEGEL, P. N. VENKATASUBRAMANIAN, E. MOK and A. C. WILBUR, Human leg neuromuscular diseases: P-31 MR spectroscopy. Radiology, 172: 503-508. 1989.
4) BENDALL, J. R., The effect of pre-treatment of pigs with curare on the post-mortem rate of pH fall and onset of rigor mortis in the musculature. J. Sci. Food Agric., 17: 333-338. 1966.
5) BENDALL, J. R., Post-mortem changes in muscle. in The structure and function of muscle (BOURNE, G. H., ed.). 243-309. Academic Press. New York. 1973.
6) BURT, C. T., T. GLONEK and M. BARANY, Phosphorus-31 nuclear magnetic resonance detection of unexpected phosphodiesters in muscle. Biochemistry, 15: 4850-4853. 1976.
7) CASSENS, R. G., D. N. MARPLE and G. EIKELENBOOM, Animal physiology and meat quality. Adv. Food Res., 21: 71-155. 1975.
8) GREASER, M. L., Conversion of muscle to meat. in Muscle as Food (BECHTEL, P. J., ed.). 37-102. Academic Press. Orlando. 1986.
9) HOCHACHKA, P. W. and T. P. MOMMSEN, Protons and anaerobiosis. Science, 219: 1391-1397. 1983.
10) HoULT, D. I., S. J. W. BUSBY, D. G. GADIAN, G. K. RADDA, R. E. RICHARDS and P. J. SEELEY, Observation of tissue metabolites using 31P nuclear magnetic resonance. Nature, 252: 285-287. 1974.
11) JORGENSEN, L. and H. GRASDALEN, 31P-NMR studies of phosphate metabolites in intact red and white swimming muscles of cod (Gadus Morhua L.). Comp. Biochem. Physiol., 84B: 447-450. 1986.
12) KASTENSCHMIDT, L. L, W. G. HOEKSTRA and E. J. BRISKEY, Glycolytic intermediate and cofactors in "fast- and slow-glycolyzing" muscles of the pig. J. Food Sci., 33: 151-158. 1968.
13) MANABE, N., T. ISHIBASHI and H. MIYAMOTO, Slow -twitch myofibers are observed in masseter muscle of the hypothalamic obese rat. Anim. Sci. Technol. (Jpn.), 64: 440-447. 1993.
14) MEYER, R. A., T. R. BROWN and M. J. KUSHMERICK. Phosphorus nuclear magnetic resonance of fast- and slow-twitch muscle. Am. J. Physiol., 248: C279-287. 1985.
15) MOON, R. B. and J. H. RICHARDS, Determination of intracellular pH by 31P magnetic resonance. J. Biol. Chem., 248: 7276-7278. 1973.
16) RENOU, J. P., P. CANIONI, P. GATELIER, C. VALIN and P. J. COZZONE, Phosphorus-31 nuclear magnetic resonance study of post mortem catabolism and intracellular pH in intact excised rabbit muscle. Biochimie, 68: 543-554. 1986.
17) SATRUSTEGUI, J., H. BERKOWITZ, B. BODEN, E. DONLON, A. MCLOUGHLIN, J. MARIS, R. WARNELL and B. CHANCE, An in vivo phosphorus nuclear magnetic resonance study of the variations with age in the phosphodiesters' content of humam muscle. Mechanism Ageing Development, 42: 105-114. 1988.
18) SCHMIDT, G., L. HECHT, P. FALLOT, L. GREENBAUM and S. J. THANNHAUSER, The amounts of glycerylphosphorylcholine in some mammalian tissues. J. Biol. Chem., 197: 601-609. 1952.
19) SEELEY, P. J., S. J. M. BUSBY, D. G. GADIAN, G. K. RADDA and R. E. RICHARDS, A new approach to metabolite compartmentation in muscle. Biochem. Soc. Trans., 4: 62-64. 1976.
20) TARRANT, P. J. V., P. V. J. HEGARTY and J. V. MCLOUGHLIN, A study of the high energy phosphates and anaerobic glycolysis in the red and white fibres of porcine semitendinosus muscle. Proc. Royal Ir. Acad., 72 (C): 229-251. 1972.
21) UHRIN, P. and T. LIPTAJ, 31P NMR study of phosphorus metabolites in fast and slow muscles. Int. J. Biochem., 22: 1133-1138. 1990.
22) UHRIN, P. and T. LIPTA,1, 31P NMR study of postmortem metabolism in porcine and bovine muscles. Gen. Physiol. Biophys., 10: 83-93. 1991.
訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル 訂正内容: -
訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報 訂正内容: Wrong : 1) AZUMA, Y., N. MANABE, F. KAWAI, M. KANAMORI and H. MIYAMOTO. Phosphorus-31 nuclear magnetic resonance study of energy metabolism in intact slow- and fast-twitch muscles of rat. J. Anim. Sci., 72: 103-108. 1994.
2) BARANY, M. and T. GLONEK, Phosphorus-31 nuclear magnetic resonance of contractile systems. Methods Enzymol., 85: 624-676. 1982.
3) BARANY, M., I. M. SIEGEL, P. N. VENKATASUBRAMANIAN, E. MOK and A. C. WILBUR, Human leg neuromuscular diseases: P-31 MR spectroscopy. Radiology, 172: 503-508. 1989.
4) BENDALL, J. R., The effect of pre-treatment of pigs with curare on the post-mortem rate of pH fall and onset of rigor mortis in the musculature. J. Sci. Food Agric., 17: 333-338. 1966.
5) BENDALL, J. R., Post-mortem changes in muscle. in The structure and function of muscle (BOURNE, G. H., ed.). 243-309. Academic Press. New York. 1973.
6) BURT, C. T., T. GLONEK and M. BARANY, Phosphorus-31 nuclear magnetic resonance detection of unexpected phosphodiesters in muscle. Biochemistry, 15: 4850-4853. 1976.
7) CASSENS, R. G., D. N. MARPLE and G. EIKELENBOOM, Animal physiology and meat quality. Adv. Food Res., 21: 71-155. 1975.
8) GREASER, M. L., Conversion of muscle to meat. in Muscle as Food (BECHTEL, P. J., ed.). 37-102. Academic Press. Orlando. 1986.
9) HOCHACHKA, P. W. and T. P. MOMMSEN, Protons and anaerobiosis. Science, 219: 1391-1397. 1983.
10) HoULT, D. I., S. J. W. BUSBY, D. G. GADIAN, G. K. RADDA, R. E. RICHARDS and P. J. SEELEY, Observation of tissue metabolites using 31P nuclear magnetic resonance. Nature, 252: 285-287. 1974.
11) JORGENSEN, L. and H. GRASDALEN, 31P-NMR studies of phosphate metabolites in intact red and white swimming muscles of cod (Gadus Morhua L.). Comp. Biochem. Physiol., 84B: 447-450. 1986.
12) KASTENSCHMIDT, L. L, W. G. HOEKSTRA and E. J. BRISKEY, Glycolytic intermediate and cofactors in "fast- and slow-glycolyzing" muscles of the pig. J. Food Sci., 33: 151-158. 1968.
13) MANABE, N., T. ISHIBASHI and H. MIYAMOTO, Slow -twitch myofibers are observed in masseter muscle of the hypothalamic obese rat. Anim. Sci. Technol. (Jpn.), 64: 440-447. 1993.
14) MEYER, R. A., T. R. BROWN and M. J. KUSHMERICK. Phosphorus nuclear magnetic resonance of fast- and slow-twitch muscle. Am. J. Physiol., 248: C279-287. 1985.
15) MOON, R. B. and J. H. RICHARDS, Determination of intracellular pH by 31P magnetic resonance. J. Biol. Chem., 248: 7276-7278. 1973.
16) RENOU, J. P., P. CANIONI, P. GATELIER, C. VALIN and P. J. COZZONE, Phosphorus-31 nuclear magnetic resonance study of post mortem catabolism and intracellular pH in intact excised rabbit muscle. Biochimie, 68: 543-554. 1986.
17) SATRUSTEGUI, J., H. BERKOWITZ, B. BODEN, E. DONLON, A. MCLOUGHLIN, J. MARIS, R. WARNELL and B. CHANCE, An in vivo phosphorus nuclear magnetic resonance study of the variations with age in the phosphodiesters' content of humam muscle. Mechanism Ageing Development, 42: 105-114. 1988.
18) SCHMIDT, G., L. HECHT, P. FALLOT, L. GREENBAUM and S. J. THANNHAUSER, The amounts of glycerylphosphorylcholine in some mammalian tissues. J. Biol. Chem., 197: 601-609. 1952.
19) SEELEY, P. J., S. J. M. BUSBY, D. G. GADIAN, G. K. RADDA and R. E. RICHARDS, A new approach to metabolite compartmentation in muscle. Biochem. Soc. Trans., 4: 62-64. 1976.
20) TARRANT, P. J. V., P. V. J. HEGARTY and J. V. MCLOUGHLIN, A study of the high energy phosphates and anaerobic glycolysis in the red and white fibres of porcine semitendinosus muscle. Proc. Royal Ir. Acad., 72 (C): 229-251. 1972.
21) UHRIN, P. and T. LIPTAJ, 31P NMR study of phosphorus metabolites in fast and slow muscles. Int. J. Biochem., 22: 1133-1138. 1990.
22) UHRIN, P. and T. LIPTA,1, 31P NMR study of postmortem metabolism in porcine and bovine muscles. Gen. Physiol. Biophys., 10: 83-93. 1991.
Right : 1) AZUMA, Y., N. MANABE, F. KAWAI, M. KANAMORI and H. MIYAMOTO. Phosphorus-31 nuclear magnetic resonance study of energy metabolism in intact slow- and fast-twitch muscles of rat. J. Anim. Sci., 72: 103-108. 1994.
2) BARANY, M. and T. GLONEK, Phosphorus-31 nuclear magnetic resonance of contractile systems. Methods Enzymol., 85: 624-676. 1982.
3) BARANY, M., I. M. SIEGEL, P. N. VENKATASUBRAMANIAN, E. MOK and A. C. WILBUR, Human leg neuromuscular diseases: P-31 MR spectroscopy. Radiology, 172: 503-508. 1989.
4) BENDALL, J. R., The effect of pre-treatment of pigs with curare on the post-mortem rate of pH fall and onset of rigor mortis in the musculature. J. Sci. Food Agric., 17: 333-338. 1966.
5) BENDALL, J. R., Post-mortem changes in muscle. in The structure and function of muscle (BOURNE, G. H., ed.). 243-309. Academic Press. New York. 1973.
6) BURT, C. T., T. GLONEK and M. BARANY, Phosphorus-31 nuclear magnetic resonance detection of unexpected phosphodiesters in muscle. Biochemistry, 15: 4850-4853. 1976.
7) CASSENS, R. G., D. N. MARPLE and G. EIKELENBOOM, Animal physiology and meat quality. Adv. Food Res., 21: 71-155. 1975.
8) GREASER, M. L., Conversion of muscle to meat. in Muscle as Food (BECHTEL, P. J., ed.). 37-102. Academic Press. Orlando. 1986.
9) HOCHACHKA, P. W. and T. P. MOMMSEN, Protons and anaerobiosis. Science, 219: 1391-1397. 1983.
10) HoULT, D. I., S. J. W. BUSBY, D. G. GADIAN, G. K. RADDA, R. E. RICHARDS and P. J. SEELEY, Observation of tissue metabolites using 31P nuclear magnetic resonance. Nature, 252: 285-287. 1974.
11) JORGENSEN, L. and H. GRASDALEN, 31P-NMR studies of phosphate metabolites in intact red and white swimming muscles of cod (Gadus Morhua L.). Comp. Biochem. Physiol., 84B: 447-450. 1986.
12) KASTENSCHMIDT, L. L, W. G. HOEKSTRA and E. J. BRISKEY, Glycolytic intermediate and cofactors in "fast- and slow-glycolyzing" muscles of the pig. J. Food Sci., 33: 151-158. 1968.
13) MANABE, N., T. ISHIBASHI and H. MIYAMOTO, Slow -twitch myofibers are observed in masseter muscle of the hypothalamic obese rat. Anim. Sci. Technol. (Jpn.), 64: 440-447. 1993.
14) MEYER, R. A., T. R. BROWN and M. J. KUSHMERICK. Phosphorus nuclear magnetic resonance of fast- and slow-twitch muscle. Am. J. Physiol., 248: C279-287. 1985.
15) MOON, R. B. and J. H. RICHARDS, Determination of intracellular pH by 31P magnetic resonance. J. Biol. Chem., 248: 7276-7278. 1973.
16) RENOU, J. P., P. CANIONI, P. GATELIER, C. VALIN and P. J. COZZONE, Phosphorus-31 nuclear magnetic resonance study of post mortem catabolism and intracellular pH in intact excised rabbit muscle. Biochimie, 68: 543-554. 1986.
17) SATRUSTEGUI, J., H. BERKOWITZ, B. BODEN, E. DONLON, A. MCLOUGHLIN, J. MARIS, R. WARNELL and B. CHANCE, An in vivo phosphorus nuclear magnetic resonance study of the variations with age in the phosphodiesters' content of humam muscle. Mechanism Ageing Development, 42: 105-114. 1988.
18) SCHMIDT, G., L. HECHT, P. FALLOT, L. GREENBAUM and S. J. THANNHAUSER, The amounts of glycerylphosphorylcholine in some mammalian tissues. J. Biol. Chem., 197: 601-609. 1952.
19) SEELEY, P. J., S. J. M. BUSBY, D. G. GADIAN, G. K. RADDA and R. E. RICHARDS, A new approach to metabolite compartmentation in muscle. Biochem. Soc. Trans., 4: 62-64. 1976.
20) TARRANT, P. J. V., P. V. J. HEGARTY and J. V. MCLOUGHLIN, A study of the high energy phosphates and anaerobic glycolysis in the red and white fibres of porcine semitendinosus muscle. Proc. Royal Ir. Acad., 72 (C): 229-251. 1972.
21) UHRIN, P. and T. LIPTAJ, 31P NMR study of phosphorus metabolites in fast and slow muscles. Int. J. Biochem., 22: 1133-1138. 1990.
22) UHRIN, P. and T. LIPTA,1, 31P NMR study of postmortem metabolism in porcine and bovine muscles. Gen. Physiol. Biophys., 10: 83-93. 1991.
訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル 訂正内容: -
訂正日: 2008/03/10 訂正理由: - 訂正箇所: 論文抄録 訂正内容: 訂正前 : 生体総織のエネルギ-代謝状態に関する情報を破壊的かつ経時的に得ることが可能な31P核磁気共鳴法(NMP法)により,生理学的•生化学的特性を異にする2種類のヤギ骨格筋(棘上筋および大腿筋膜張筋)の屠殺後の高エネルギーリン酸化合物の消長を経時的に調べた.また,ウサギ,ブタなどの限られた動物の一部の骨格筋に存在することが報告されているglycerophosphorylcholine (GPC)がヤ
ギの筋肉で見出せるかどうかを検討した.雌性ザーネン種成ヤギ(6頭)をペントバルビタール麻酔下に放血屠殺し,直ちに筋肉を採取した.筋肉を整形後,直径10mmのNMR試料管に挿入し,屠殺後1,2,3,4,5,6,正2および24時間の各時点で31P NMR測定を行ない,クレアチンリン酸,ATPの経時
減少および細胞内pHの変化を調べた.測定はすべて25±1°Cで行なった.併せて,両筋の組織化学的特性の差異を明らかにするため,凍結横断切片を作成し,免疫および酵素組織化学的方法により筋線維型組成を明らかにした.主にI型筋線維(slow-twitch)から構成される棘上筋とII型筋線維(fast-twitch)優勢の大腿筋膜張筋では,屠殺後の高エネルギーリン酸化合物の経時減少に顕著な差が認められた.すなわち,棘上筋では大腿筋膜張筋と比べてクレアチンリン酸およびATPの減少•消失が早かった.限られた家畜のI型筋線維主体の筋で存在が報告されているGPCがヤギ棘上筋にも存在
することが31P NMR測定により明らかとなった.大腿筋張筋ではこの物質は認められなかった.細胞内PHの低下パターンにも2種類の筋肉間で差が認められた.すなわち,屠殺直後と屠殺24時間後の時点では両者間に差がないものの,棘上筋の細胞内pH低下が早く,屠殺後2～6時間の各時点で細胞内
pHに有意な差が認あられた.
訂正後 : 生体総織のエネルギ-代謝状態に関する情報を破壊的かつ経時的に得ることが可能な31P核磁気共鳴法(NMP法)により,生理学的•生化学的特性を異にする2種類のヤギ骨格筋(棘上筋および大腿筋膜張筋)の屠殺後の高エネルギーリン酸化合物の消長を経時的に調べた.また,ウサギ,ブタなどの限られた動物の一部の骨格筋に存在することが報告されているglycerophosphorylcholine (GPC)がヤギの筋肉で見出せるかどうかを検討した.雌性ザーネン種成ヤギ(6頭)をペントバルビタール麻酔下に放血屠殺し,直ちに筋肉を採取した.筋肉を整形後,直径10mmのNMR試料管に挿入し,屠殺後1,2,3,4,5,6,正2および24時間の各時点で31P NMR測定を行ない,クレアチンリン酸,ATPの経時減少および細胞内pHの変化を調べた.測定はすべて25±1°Cで行なった.併せて,両筋の組織化学的特性の差異を明らかにするため,凍結横断切片を作成し,免疫および酵素組織化学的方法により筋線維型組成を明らかにした.主にI型筋線維(slow-twitch)から構成される棘上筋とII型筋線維(fast-twitch)優勢の大腿筋膜張筋では,屠殺後の高エネルギーリン酸化合物の経時減少に顕著な差が認められた.すなわち,棘上筋では大腿筋膜張筋と比べてクレアチンリン酸およびATPの減少•消失が早かった.限られた家畜のI型筋線維主体の筋で存在が報告されているGPCがヤギ棘上筋にも存在することが31P NMR測定により明らかとなった.大腿筋張筋ではこの物質は認められなかった.細胞内PHの低下パターンにも2種類の筋肉間で差が認められた.すなわち,屠殺直後と屠殺24時間後の時点では両者間に差がないものの,棘上筋の細胞内pH低下が早く,屠殺後2～6時間の各時点で細胞内pHに有意な差が認あられた.
訂正日: 2008/03/10 訂正理由: - 訂正箇所: 所属機関情報 訂正内容: 訂正前 :1) 環境科学総合研究所2) 京都大学農学部
訂正後 :1) (財)環境科学総合研究所2) 京都大学農学部
訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル 訂正内容: -
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