Cholinesterase-based Biosensor Using Surface Photovoltage Technique

抄録

The new measurement method of Surface Photovoltage (SPV) technique was applied to the fabrication of a novel type cholinesterase-based biosensor. Cholinesterases (butyrylcholine esterase (BuChE) and acetylcholine esterase (AChE)) have been immobilized directly onto the semiconductor surface to measure the concentration of enzyme substrates, via pH changes. The detection limits of the substrates were 9.0×10⁻⁷ M, 2.7×10⁻⁶ M and 4.1×10⁻⁶ M for butyrylthiocholine iodide, acetylcholine iodide and acetylcholine chloride, respectively. The analytical possibilities were examined from the results of the inhibiting actions exerted in the presence of alkaloids such as physostigmine sulfate and neostigmine bromide on BuChE.