Comparison between Bacterial Diversity of Aged and Aging Pit Mud from Luzhou-flavor Liquor Distillery

Abstract

Bacterial diversity of the aged and aging pit mud from Luzhou-flavor liquor distillery was investigated and compared by molecular methods. Two bacteria-specific 16S rRNA gene clone libraries were constructed and analyzed using the amplified rRNA gene restriction analysis. A total of 172 clones were studied and 23 operational taxonomic units (OTUs) were obtained. Firmicutes and Chloroflexi predominated in the aged pit mud while Firmicutes and Bacteroidetes predominated in the aging pit mud. What is more, Chloroflexi and Actinobacteria were only detected in the aged pit mud. Additionally, the results of quantitative real time PCR (qPCR) showed that the quantity of Actinobacteria in the aged pit mud was 29 times as much as in the aging pit mud (P < 0.05). The results have compared the bacterial diversity of the aged and aging pit mud for the first time and maybe useful to further understand the microorganism of pit mud.