Anti-apoptotic Effect of Grape Seed Proanthocyanidin Extract on Cisplatin-induced Apoptosis in Rat Testis

Abstract

This study was to investigate the possible anti-apoptotic effect of grape seed proanthocyanidin extract (GSPE) on cisplatin (CDDP)-induced testicular apoptosis in male rats. The control group was treated with physiological saline, the GSPE (400 mg/kg) group, GSPE (200 mg/kg) + CDDP group and GSPE (400 mg/kg) + CDDP group were received GSPE orally at corresponding dose respectively, for 15 consecutive days, starting 10 days before a single intraperitoneal dose of CDDP (7 mg/kg) in CDDP group and GSPE + CDDP groups. Administration of CDDP significantly reduced the weight of testis and epididymis, sperm concentration and caused significantly increase in the apoptosis rate of testicular cells and expression of Bax and Caspase-3, decrease in the expression of Bcl-2, along with some histopathological lesions in testicular tissue. Therefore, pre-treatment of GSPE to CDDP-injected rats tend to prevent CDDP-induced lose weight of organs, testicular apoptosis and histopathological lesions. Presented data have documented GSPE have improvement effects against testicular apoptosis caused by CDDP.